Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags

Wang, Aiguo; Yoon, Sun Young; Oh, Jung‐Hwa; Jeon, Yeo-Jin; Kim, Mirang; Kim, Jeongmin; Byun, Sang-Soon; Yang, Jin Ok; Kim, Joo Heon; Kim, Dae‐Ghon; Yeom, Young‐Il; Yoo, Hyang–Sook; Kim, Yong‐Sung; Kim, Nam-Soon

doi:10.1016/j.bbrc.2006.04.175

Cited by 44 publications

(32 citation statements)

References 32 publications

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“…Indeed, the determination of alterations to the protein profiles of ICC may offer opportunities to identify tumor-specific markers. To date, proteomics studies performed using tumor tissues have mainly tried to identify proteins with a differential expression between HCC and ICC to prevent the misclassification of these pathologies (11,12). Furthermore, these studies, performed on total liver homogenates, were not appropriate to detect proteins with altered expression in tumorous cholangiocytes.…”

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confidence: 99%

Identification of Cellular Targets in Human Intrahepatic Cholangiocarcinoma Using Laser Microdissection and Accurate Mass and Time Tag Proteomics

Santos

Court

Thiers

et al. 2010

Molecular & Cellular Proteomics

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Obtaining accurate protein profiles from homogeneous cell populations in heterogeneous tissues can enhance the capability to discover protein biomarkers. In this context, methodologies to access specific cellular populations and analyze their proteome with exquisite sensitivity have to be selected. We report here the results of an investigation using a combination of laser microdissection and accurate mass and time tag proteomics. The study was aimed at the precise determination of proteome alterations in intrahepatic cholangiocarcinoma ICC, a markedly heterogeneous tumor. This cancer, which is difficult to diagnose and carries a very poor prognosis, has shown an unexplained increase in incidence over the last few years. Among a pool of 574 identified proteins, we were able to report on altered abundance patterns affecting 39 proteins conforming to a variety of potential tumorigenic pathways. The reliability of the proteomics results was confirmed by Western blot and immunohistochemistry on matched samples. Most of the proteins displaying perturbed abundances had not yet been described in the setting of ICC. These include proteins involved in cell mobility and actin cytoskeleton remodeling, which may participate in the epithelial to mesenchymal transition, a process invoked in migration and invasion of cancer cells. The biological relevance of these findings was explored using a tissue microarray. An increased abundance of vimentin was thus detected in 70% of ICC and none of the controls. These results suggest that vimentin could play a role in the aggressiveness of ICC and provide a basis for the serious outcome of this cancer. Molecular & Cellular Proteomics

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confidence: 99%

Identification of Cellular Targets in Human Intrahepatic Cholangiocarcinoma Using Laser Microdissection and Accurate Mass and Time Tag Proteomics

Santos

Court

Thiers

et al. 2010

Molecular & Cellular Proteomics

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“…However, recent studies revealed that ANXA1 expression was markedly decreased in esophageal [11] , gastric [12] , thyroid [13] , nasopharyngeal [14] , and breast carcinomas [15,16] , and correlated with tumor differentiation, metastasis, and poor outcome. Wang et al [17] investigated ANXA1 expression in 12 specimens of intrahepatic cholangiocarcinoma and 4 normal liver tissues and found that 83.3% (10/12) cases showed positive staining for ANXA1 while normal bile duct epithelium and normal liver tissues did not stain for ANXA1.…”

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confidence: 99%

Annexin-1 Downregulation Is Associated with Clinical Outcome in Chinese Patients with Hilar Cholangiocarcinoma

Wang

Zhang²,

Zheng³

et al. 2010

Eur Surg Res

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Aims: Annexin A1 (ANXA1) is a calcium- and phospholipid-binding protein and has been implicated in regulating inflammatory responses, cell proliferation and apoptosis. The evidence of its importance in carcinogenesis increased rapidly. However, the status of ANXA1 in hilar cholangiocarcinoma remains unclear. Here, we detected ANXA1 expression and determined its clinical significance in Chinese patients with hilar cholangiocarcinoma. Methods: Tissue microarray blocks containing tumor specimens obtained from 61 patients were constructed. Expression of ANXA1 in these specimens was analyzed using immunohistochemical studies. Results: ANXA1 expression was observed in 27 cases (44.3%). Loss of ANXA1 expression was significantly associated with lymph node metastases and histopathologic grade. ANXA1 expression has a significant inverse correlation with recurrence and poor survival in univariate analyses.Multivariate analyses revealed that loss of ANXA1 expression was an independent predictor for future recurrence and overall survival in patients with cholangiocarcinoma. Conclusions: Decreased expression of ANXA1 is a common event in human hilar cholangiocarcinoma and is significantly correlated with the poor outcome of patients with cholangiocarcinoma, suggesting a potential role of ANXA1 in cancer development and progression.

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“…To test whether the results from the current study were influenced by the presence of blood borne tumor cells, three databases containing gene expression profiles from primary tissues of CCA (Jinawath et al, 2006;Obama et al, 2005;Wang et al, 2006) were checked for similarities with the profile from the PBL transcriptome. No highly expressed transcripts present in primary tumor CCA tissues were found in our database and vice versa.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Profiles of Peripheral Blood Leukocytes Identify Patients with Cholangiocarcinoma and Predict Outcome

Subimerb¹,

Wongkham²,

Khuntikeo³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Cholangiocarcinoma (CCA), a slow growing but highly metastatic tumor, is highly prevalent in Northeast Thailand. Specific tests that predict prognosis of CCA remain elusive. The present study was designed to investigate whether peripheral blood leukocyte (PBL) transcriptional profiles might be of use as a prognostic test in CCA patients. Gene expression profiles of PBLs from 9 CCA and 8 healthy subjects were conducted using the Affymetrix HG_U133 Plus 2.0 GeneChip. We indentified informative PBLs gene expression profiles that could reliably distinguish CCA patients from healthy subjects. Of these CCA specific genes, 117 genes were up regulated and 60 were down regulated. The molecular and cellular functions predicted for these CCA specific genes according to the Gene Ontology database indicated differential PBL expression of host immune response and tumor progression genes (EREG, TGF β1, CXCL2, CXCL3, IL-8, and VEGFA). The expression levels of 9 differentially expressed genes were verified in 36 CCA vs 20 healthy subjects. A set of three tumor invasion related genes (PLAU, CTSL and SERPINB2) computed as "prognostic index" was found to be an independent and statistically significant predictor for CCA patient survival. The present study shows that CCA PBLs may serve as disease predictive clinically accessible surrogates for indentifying expressed genes reflective of CCA disease severity.

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Identification of intrahepatic cholangiocarcinoma related genes by comparison with normal liver tissues using expressed sequence tags

Cited by 44 publications

References 32 publications

Identification of Cellular Targets in Human Intrahepatic Cholangiocarcinoma Using Laser Microdissection and Accurate Mass and Time Tag Proteomics

Identification of Cellular Targets in Human Intrahepatic Cholangiocarcinoma Using Laser Microdissection and Accurate Mass and Time Tag Proteomics

Annexin-1 Downregulation Is Associated with Clinical Outcome in Chinese Patients with Hilar Cholangiocarcinoma

Transcriptional Profiles of Peripheral Blood Leukocytes Identify Patients with Cholangiocarcinoma and Predict Outcome

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