Identification of intranuclear inclusions is useful for the cytological diagnosis of ovarian clear cell carcinoma

Naka, Masaki; Ohishi, Yoshihiro; Kaku, Tsunehisa; Watanabe, Soichi; Tamiya, Sadafumi; Ookubo, Fumihiko; Kato, Kanefusa; Oda, Yoshinao; Sugishima, Setsuo

doi:10.1002/dc.23322

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…Currently, the frequency of NGs and ICIs in renal cell carcinoma histological specimens is 96 and 65%, respectively ( 18 ). In contrast, the frequency of ICIs in cytological specimens of ovarian cancer is 38% ( 19 ), while the frequency of ICIs in cytological specimens of thyroid papillary carcinoma cases is 88.9% ( 20 ). In terms of lung adenocarcinoma cases, Choi et al reported the frequency of either NGs or ICIs in anaplastic lymphoma kinase (ALK)-positive cases and ALK-negative cases as 21.1 and 9.9%, respectively ( 21 ).…”

Section: Discussionmentioning

confidence: 89%

Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

et al. 2018

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Nuclear size and shape are important components in the diagnosis of pathological specimens. However, a qualitative evaluation is typically applied rather than a quantitative evaluation technique. In the present study, we sought to evaluate the nuclear morphological characteristics of lung adenocarcinoma using whole-slide imaging (WSI) and computer-assisted image analysis (IA). We evaluated the nuclear characteristics of 106 cases of surgically resected lung adenocarcinoma according to Feulgen staining and immunohistochemistry (IHC) for the inner nuclear membrane protein emerin. According to the Feulgen reaction, although the nuclear area (size) of the carcinoma cells was correlated with the nuclear perimeter (NP) (R=0.8973), the nuclear staining intensity of carcinoma cells was not correlated with the nuclear area. Using emerin IHC, we used IA software that was able to detect both the NP and the emerin-stained nuclear membrane length (ENML) in the nucleus, and found that the more nuclei exhibited a longer ENML relative to the NP, the more nuclear grooves and intranuclear cytoplasmic inclusions were present. In addition, the nuclear area was correlated with the percentage of nuclei that had a longer ENML compared to the NP against the total nuclei (R=0.7759). Furthermore, the emerin low expression group showed an enlarged nuclear area (P=0.0264), elongated NP (P=0.0091), and lower shape factor (P=0.0486) compared with the normal emerin expression group. Our data indicated the usefulness of WSI and IA for pathological specimen analysis. In addition, this study is the first to report that the low expression of emerin in cancer cell results in an oval shape of nuclei and nuclear enlargement in clinical samples.

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Section: Discussionmentioning

confidence: 89%

Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

et al. 2018

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“…Nuclear pleomorphism is moderate, and nucleoli are frequently present. Intranuclear inclusions may be seen [69], as well as the presence of an extracellular hyalinized matrix, corresponding to stromal hyalinization often seen in histologic sections and cell blocks (Fig. 14) [70].…”

Section: Seromucinous Neoplasmsmentioning

confidence: 99%

“…This matrix is composed of basement material, mostly collagen IV and laminin, and can be seen in a circumferential pattern where the stroma is centrally located in a spherule-like formation ("raspberry body" or "mirror ball"), or as a large globule-like extracellular material [71]. Rare psammoma bodies can also be present [69,70].…”

Section: Seromucinous Neoplasmsmentioning

confidence: 99%

Practical Approach to the Evaluation of Malignant Peritoneal Fluids in the Setting of Gynecologic Neoplasms

Mendoza¹,

Lastra²

2022

Acta Cytologica

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Background: Evaluation of peritoneal fluid cytology, either from ascitic fluids or as result of peritoneal washings, is a fundamental aspect in the evaluation of women presenting with clinically concerning or histologically confirmed gynecologic neoplasms. Summary: Ascitic fluid samples are often the initial and only source of diagnostic material in women presenting with gynecologic malignancies, and important therapeutic decisions will result from the information provided in the cytology report. On the other hand, cytologic evaluation of peritoneal washing specimens obtained during surgical excision of a presumed gynecologic neoplasm provides crucial information to the clinical team regarding tumor staging, often with significant therapeutic implications. While recognition of high-grade tumors in either of these samples is generally straightforward, low-grade tumors and unusual neoplasms can prove to be more difficult to recognize, differentiate from benign mimics, and correctly diagnose, particularly in low-cellularity specimens. Even with high grade tumors, a mere diagnosis of “Positive for malignancy” in diagnostic ascitic fluid specimens might not suffice to guide clinical management, and the use of ancillary techniques to further and more definitively characterize the lesional cells is required. Key Messages: This review will focus on the clinically relevant issues surrounding interpretation of peritoneal fluid cytology specimens in the setting of gynecologic neoplasms, making emphasis on the salient cytomorphologic and immunocytochemical features of the various neoplastic processes, in an attempt to provide a practical yet effective guide on how to best evaluate, diagnose, and report these samples.

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“…Intranuclear inclusions are important diagnostic features in many benign and malignant neoplasms, such as adrenal cortical adenoma, thyroid papillary carcinoma, melanoma, meningioma, hepatocellular carcinoma, pancreatoblastoma, pulmonary blastoma and fetal-type adenocarcinoma, and lobular breast carcinoma [1–5] as well as recently reported ovarian serous carcinoma [2]. In renal epithelial cell tumors, the present of intranuclear inclusions has been reported in all three major subtypes, including chromophobe, papillary and ccRCC [3–5].…”

Section: Introductionmentioning

confidence: 99%

Intranuclear inclusions in conventional clear cell Renal Cell Carcinoma (RCC): A case report and review of the literature

et al. 2018

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Intranuclear inclusions are important diagnostic features in many benign and malignant neoplasms. It has also been identified in major epithelial subtypes of renal cell carcinomas (RCCs), particularly in the chromophobe RCC. However, the finding in ccRCC has not been well studied. The finding of intranuclear inclusions may cause diagnostic difficulty, particularly in metastatic lesions. Herein, we reported a case of ccRCC with prominent intranuclear inclusions. The tumor also metastasized to local lymph nodes. Furthermore, in contrast to previous publications, we also found that intranuclear inclusions were immunoreactive with anti-PAX8 (paired box8) antibody. The potential diagnostic and clinical implications of intranuclear inclusions in ccRCC need to be addressed.

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Identification of intranuclear inclusions is useful for the cytological diagnosis of ovarian clear cell carcinoma

Abstract: The identification of intranuclear inclusions, in particular a high frequency and multiple intranuclear inclusions in a single nucleus, is useful for the cytological diagnosis of CCC. Furthermore, these results may be applicable to ascites cytology.

Cited by 5 publications

References 24 publications

Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

Image analysis of the nuclear characteristics of emerin protein and the correlation with nuclear grooves and intranuclear cytoplasmic inclusions in lung adenocarcinoma

Practical Approach to the Evaluation of Malignant Peritoneal Fluids in the Setting of Gynecologic Neoplasms

Intranuclear inclusions in conventional clear cell Renal Cell Carcinoma (RCC): A case report and review of the literature

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