Identification of ISSR and RAPD markers linked to yield traits in bread wheat under normal and drought conditions

Abstract

Genetic variability and identification of some molecular markers were studied in twenty promising lines of wheat using agronomic traits, ISSR (inter simple sequences repeats) and RAPD (random amplified polymorphic DNA) markers. Significant variation was evidenced in all agronomic traits. The lines proved to be superior to the check cultivar Sahel1 in yield and its component traits. Lines L2, L7 and L8 were the best in most yield component traits in both seasons. Moreover, Lines L2, L4, L5, L7 and L8 showed drought tolerance by which they displayed high performance in agronomic traits as well as a low drought susceptibility index. The percentage of polymorphism was 39.3% and 53.2% for ISSRs and RAPDs, respectively. UBC-881 belonged to penta-nucleotide repeat sequences (GGGTG) that produced the highest level of polymorphism, while UBC-846 belonged to di-nucleotide repeat sequences (CA) that produced the lowest level of polymorphism. Genetic similarities among wheat lines based on ISSR and RAPD markers ranged from 0.81 to 1.00 and from 0.86 to 0.98, respectively. There was a low average of PIC (polymorphism information content) values which were 0.10 (ISSR) and 0.15 (RAPD). The RAPD technique exhibited a higher marker index (MI = 0.69) compared to ISSR (MI = 0.43). There was insignificant correlation between ISSR and RAPD data (0.168, p > 0.05). There were two markers (UBC-881450bp and OPF-10540bp), on each of which two traits regressed significantly. The associated markers each explained a maximum regression of 18.92–34.95% of the total available variation for individual associated traits.

Characterization of maize genotypes for genetic diversity on the basis of inter simple sequence repeats

“…PIC values, as obtained in this study, have been extensively used in various genetic diversity analyses. The moderate to high PIC values obtained here indicate the importance of DNA markers in germplasm analysis, gene mapping, and molecular breeding (Khaled et al, 2015). The PIC values obtained in this study also show the diverse nature of these maize genotypes.…”

RAPD and ISSR marker assessment of genetic diversity in Citrullus colocynthis (L.) Schrad: a unique source of germplasm highly adapted to drought and high-temperature stress

“…This average polymorphic band per primer is closely related with earlier studies as 6.83 polymorphic fragments per primer in Ricinus communis as (Gajera et al 2010), 7.6 PB/P in Pistacia khinjuk (Karimi et al 2012) and 7.6 PB/P in Iranian Pistachios (Aliakbarkhani et al 2015). However, earlier observations also reflect less number of polymorphic band per primer in various plant species such as, 2.9 PB/P in bread wheat (Khaled et al 2015), 3.58 PB/ P in Cucumis sativus (manohar et al 2013), 4.17 PB/P in Citrullus lanatus (Solmaz et al 2010) and in barley 5.67 PB/P (Guasmi et al 2012). Contrary to this, Fig.…”

“…However, earlier observations of less average polymorphic band per primer from the present study were also reported in different plant species using ISSR markers viz. 2.8 PB/P in Benincasa hispida (Verma et al 2007), 4.4 PB/P in sugarcane Germplasm (Khaled et al 2015) and 5.12 PB/P in chickpea (Rao et al 2007). Contrary to this, high average value of the present work has been reported in other plant species such as, 17.78 PB/P in Primula obconica (Nan et al 2003), 16.87 PB/P in Vigna radiata (Singh et al 2012), 15 PB/P in Artemisia herba-alba (Mohsen and Ali 2008), 14.4 PB/P in sugar beet (Izzatullayeva et al 2014), 13 PB/P in Miscanthus (Hodkinson et al 2002), 12.6 PB/P in Citrullus lanatus (Dje et al 2010) and 11.6 PB/P in Cucumis melo (Stepansky et al 1999).…”

In vitro screening of durum wheat against water-stress mediated through polyethylene glycol

“…Environmental conditions in agricultural settings are highly variable, leading to suboptimal crop yields and survival rates. The frequency and intensity of environmental extremes are expected to increase with climate change [24]. How plants cope with drought stress is a topic of an intense debate.…”