Identification of ISSR Markers for Studying the Biodiversity of Bulgarian Representatives of Genus<i>Orobanche</i>Subsection<i>Minores</i>

Stoyanov, Kiril; Gevezova, Maria; Denev, Iliya

doi:10.5504/bbeq.2011.0139

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…The primer 807 recorded the highest value of 0.91 in this study, showing high polymorphism, which is consistent with the results of genetic diversity in wheat [ 32 ]. Additionally, the values of MI were from 1.66 to 12.04 with an average of 7.38, which are in accordance with the outcomes of Akhtar et al [ 35 ] and Ghehsareh et al [ 36 ]. Therefore, the primers tested in this study were effective and informative.…”

Section: Discussionsupporting

confidence: 89%

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Zhang,

Du,

Wang

et al. 2024

Mol Biol Rep

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Background As a holoparasitic weed, broomrape has seriously threatened the production of economically important crops, such as melon, watermelon, processed tomato, and sunflower, in Xinjiang in recent years. However, the distribution and genetic diversity of broomrape populations in Xinjiang are not clear at present, which hinders their prevention and control. The purpose of this study was to identify the main species and the genetic differentiation structure of the broomrape population in Xinjiang. Methods and results In the present study, 93 samples from different geographic regions of Xinjiang were collected to identify the species based on ITS and plastid rps2 regions, and the samples were also used to analyze the genetic diversity based on ISSR markers. The results showed that broomrape is not monophyletic in Xinjiang and consists of two major clades (Orobanche cf. aegyptiaca and O. cernua) and three subclades (O. cf. aegyptiaca var. tch, O. cf. aegyptiaca var. klz, and O. cernua.var. alt) based on phylogenetic analysis. Furthermore, the results of the genetic diversity analysis indicated that the average polymorphic information content and marker index were high values of 0.58 and 7.38, respectively, showing the efficiency of the ISSR markers in detecting polymorphism among the broomrape population studied. Additionally, the 11 selected primers produced 154 repeatable polymorphic bands, of which 150 were polymorphic. The genetic diversity of the samples was 37.19% within populations and 62.81% among the populations, indicating that the main genetic differentiation occurred among the populations. There was less gene exchange between populations, with a gene flow index (Nm) of 0.2961 (< 1). The UPGMA dendrogram indicated that most populations with similar geographical conditions and hosts were clustered first, and then all samples were separated into two major groups and seven subclusters. Conclusion The broomrapes are mainly O. cf. aegyptiaca and O. cernua in Xinjiang, which were separated into two major groups and seven subclusters based on ISSR markers. Our results provide a theoretical basis for breeding broomrape-resistant varieties.

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Section: Discussionsupporting

confidence: 89%

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Zhang,

Du,

Wang

et al. 2024

Mol Biol Rep

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“…All samples in the second cluster were single, and their host ranges were narrow. The same and near geographic origins mostly gathered together, which is similar to the results of Stoyanov et al [24] . Interestingly, sample P22 came from the 163rd regimen, and the host was chili, which was shorter than other samples of the same origin in terms of morphological characteristics; however, it clustered together with samples of the same origin.…”

Section: Discussionsupporting

confidence: 89%

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Zhang,

Du,

Wang

et al. 2023

Preprint

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Background: As a holoparasitic weed, broomrape has seriously threatened the production of economically important crops, such as melon, watermelon, processed tomato, and sunflower, in the Xinjiang Province in recent years. However, the distribution and genetic diversity of broomrape populations in Xinjiang are not clear at present, which hinders their prevention and control process. The purpose of this study is to identify the main species and the genetic differentiation structure of the broomrape population in Xinjiang province. Methods and Results In the present study, 93 samples from different geographic regions of Xinjiang were collected to identify the species based on ITS and plastid rps2 regions, and the samples were also used to analyze the genetic diversity based on ISSR markers. The results showed that broomrape is not monophyletic in Xinjiang and consists of two major clades (Orobanche cf. aegyptiaca and O. cernua) and three subclades (O. cf. aegyptiaca var. tch, O. cf. aegyptiaca var. klz, O. cernua.var. alt) by phylogenetic analysis based on ITS and rps2. Furthermore, the results of the genetic diversity analysis indicated that the 11 selected primers produced 154 repeatable polymorphic bands, of which 150 were polymorphic. The genetic diversity of the samples was 37.19% within populations and 62.81% among the populations, indicating that the main genetic differentiation occurred among the populations. There was less gene exchange between populations, with a gene flow index (Nm) of 0.2961 (<1). The UPGMA dendrogram indicated that most populations with similar geographical conditions and hosts were clustered first, and then all samples were separated into two major groups and seven subclusters. Conclusion The broomrapes are mainly O. cf. aegyptiacaand O. cernua in Xinjiang province, which were separated into two major groups and seven subclusters furtherly based on ISSR markers. This is the first study to clarify genetic diversity and population distribution of broomrape in Xinjiang. Our results provide a theoretical basis for the prevention, control, and breeding of broomrape-resistant varieties.

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“…ISSR markers have been used to assess the genetic diversity, taxonomic and phylogenetic relationships within various species of Orobanche and closely related species from the same section. They have been employed to distinguish between different populations of O. hederae, O. cernua, or O. cumana (Benharrat et al, 2002), as well as to detect inter-and intrapopulation polymorphism in O. cumana from different geographical regions, including Bulgaria (Stoyanov et al, 2012;Duca and Bivol, 2022), Spain (Román et al, 2002), Republic of Moldova (Duca et al, 2020;Clapco et al, 2020), China (Shi et al, 2019), Romania (Benharrat et al, 2002), Turkey (Duca and Bivol, 2023), Egypt (Sharawy and Karakish, 2015;Abdalla et al, 2016), Russia, Ukraine and others.…”

Section: Introduction Introduction Introduction Introductionmentioning

confidence: 99%

Analysis of genetic relationships between broomrape populations from different countries using ISSR markers

DUCA,

BIVOL,

MUTU

et al. 2024

Not Bot Horti Agrobo

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Orobanche cumana, commonly known as sunflower broomrape, poses a significant risk to sunflower cultivation in areas located along the Black Sea and across Europe. A study was conducted to analyze the genetic diversity and differentiation among populations of this parasitic plant originating from Bulgaria, Turkey, Republic of Moldova, and Romania. A total of 269 individuals from 23 populations were genotyped using 13 ISSR markers. The frequency distribution of alleles in the broomrape populations from Romania, Turkey and Bulgaria is more homogeneous than that from Moldavian populations. However, according to genetic diversity parameters O. cumana populations from Moldova and Turkey were more variable (total gene diversity Ht=0.188; 0.214), than those from Bulgaria and Romania (Ht=0.112; 0.067). The highest values of gene diversity within populations (Hs = 0.138) were found in Turkish broomrape. The value of Gst ranged between 0.359-0.516, indicating a very high level of genetic differentiation among populations. These results are consistent with low Nm values (0.468-0.895). Pairwise differentiation index (PhiPT) and Nei’s unbiased measures of genetic distance (GD) showed similar patterns, indicating a maximum (PhiPT=0.500; GD=0.261) and, respectively, minimum (PhiPT=0.238; GD=0.079) values between broomrape from Turkey and Romania, respectively, Romania and Moldova. Dendrogram constructed using the UPMGA method based on Nei's genetic distances and Pearson's dissimilarity clustered together Moldavian and Romanian broomrape and grouped in two separate clusters populations belonging to Bulgaria and Turkey. The PCA analysis confirmed the results of UPGMA clusterization. Overall, both methods suggest that the groupings of broomrape are partly determined by its geographical origin, as well as by the genetic differences and similarities accumulated over time, and are not related to virulence.

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Identification of ISSR Markers for Studying the Biodiversity of Bulgarian Representatives of GenusOrobancheSubsectionMinores

Cited by 5 publications

References 19 publications

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Identification and genetic diversity analysis of broomrape in Xinjiang, China

Analysis of genetic relationships between broomrape populations from different countries using ISSR markers

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