Identification of Japanese Lymantria species (Lepidoptera: Lymantriidae) based on PCR–RFLP analysis of mitochondrial DNA

Arimoto, Makoto; Iwaizumi, Ren

doi:10.1007/s13355-013-0235-x

Cited by 21 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Particularly in advanced technologies like PCR–RFLP methods3 and DNA barcodes33, these similarities and differences between L. salicis and other insects could be used as potential markers in species identification, especially the differences.…”

Section: Discussionmentioning

confidence: 99%

“…However, the moth appears mainly in June to August, the rest of its life go through egg and larva stages (which has no easily identifying morphological features), requiring eggs and larvae to be reared to adult stage for identification, which is time consuming and labor intensive. Molecular methods for identification are under development, including polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP)3. Most previous work on L. salicis has focused on sex pheromone synthesis4, or the nuclear polyhedrosis virus that infects larvae5.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the Complete Mitochondrial Genome of Leucoma salicis (Lepidoptera: Lymantriidae) and Comparison with Other Lepidopteran Insects

Sun

Wang

Wei

et al. 2016

Sci Rep

View full text Add to dashboard Cite

The complete mitochondrial genome (mitogenome) of Leucoma salicis (Lepidoptera: Lymantriidae) was sequenced and annotated. It is a circular molecule of 15,334 bp, containing the 37 genes usually present in insect mitogenomes. All protein-coding genes (PCGs) are initiated by ATN codons, other than cox1, which is initiated by CGA. Three of the 13 PCGs had an incomplete termination codon, T or TA, while the others terminated with TAA. The relative synonymous codon usage of the 13 protein-coding genes (PCGs) was consistent with those of published lepidopteran sequences. All tRNA genes had typical clover-leaf secondary structures, except for the tRNASer (AGN), in which the dihydrouridine (DHU) arm could not form a stable stem-loop structure. The A + T-rich region of 325 bp had several distinctive features, including the motif ‘ATAGA’ followed by an 18 bp poly-T stretch, a microsatellite-like (AT)7 element, and an 11-bp poly-A present immediately upstream of tRNAMet. Relationships among 32 insect species were determined using Maximum Likelihood (ML), Neighbor Joining (NJ) and Bayesian Inference (BI) phylogenetic methods. These analyses confirm that L. salicis belongs to the Lymantriidae; and that Lymantriidae is a member of Noctuoidea, and is a sister taxon to Erebidae, Nolidae and Noctuidae, most closely related to Erebidae.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Characterization of the Complete Mitochondrial Genome of Leucoma salicis (Lepidoptera: Lymantriidae) and Comparison with Other Lepidopteran Insects

Sun

Wang

Wei

et al. 2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…). Two subsequent studies concurred with Pogue & Schaefer's revised status for albescens and postalba : while the conclusions of one were based on morphological criteria alone (Arimoto & Iwaizumi, ), those of the other study were based on the presence of a single SNP within the cytochrome c oxydase 2 (COII) gene, sequenced from only one specimen each of albescens and postalba (Arimoto & Iwaizumi, ). However, the same authors subsequently conducted a more thorough molecular assessment, based on partial COI and COII sequences, and concluded that the two taxa could not be distinguished from one another on the basis of these mt genomic data (Arimoto & Iwaizumi, ).…”

Section: Introductionmentioning

confidence: 67%

Reassessment of the status of Lymantria albescens and Lymantria postalba (Lepidoptera: Erebidae: Lymantriinae) as distinct ‘Asian gypsy moth’ species, using both mitochondrial and nuclear sequence data

Djoumad

Nisole

Stewart

et al. 2019

Systematic Entomology

View full text Add to dashboard Cite

For regulatory purposes, the name ‘Asian gypsy moth’ refers to a group of closely related Asian Lymantria species and subspecies whose female moths display flight capability, a trait believed to confer enhanced invasiveness relative to the European gypsy moth, Lymantria dispar dispar, whose females are flightless. Lymantria albescens and Lymantria postalba are Asian gypsy moths occurring in the southern Ryukyu Islands and in the northern Ryukyu and adjacent Kyushu and Shikoku Islands of Japan, respectively. Although once considered subspecies of L. dispar, their status as distinct species, relative to the latter, is now well established. While postalba was subsequently considered a subspecies of L. albescens, largely on the basis of differences in forewing ground colour in males, both taxa were later given distinct species status by Pogue & Schaefer (2007) following their revision of the genus Lymantria. Here, we re‐examined the validity of this revised status through the sequencing of a large portion of the mitochondrial genome (c. 60%) and multiple nuclear marker genes [elongation factor 1‐alpha (Ef‐1α), wingless (Wgl), internal transcribed spacer 2 (ITS‐2), ribosomal protein S5 (RpS5)] in representative specimens of both taxa and other Lymantria species, including L. monacha, L. xylina, L. mathura and members of the L. dispar + L. umbrosa clade. A comparison of the number of substitutions in these genomic regions among the taxa we considered showed lower or equivalent variation between L. albescens and L. postalba compared with subspecies of L. dispar, for mitochondrial and nuclear sequences, respectively. This finding was reflected in the maximum likelihood trees generated independently for mitochondrial and nuclear data, where L. albescens and L. postalba formed, in both analyses, a short‐branch sister clade basal to the L. dispar + L. umbrosa clade. We further sequenced three markers [cytochrome c oxydase 1 (COI), EF‐1α, Wgl] in multiple L. albescens–L. postalba specimens collected along a south‐to‐north transect across the Ryukyu Arc and observed no clear distinction among the sampled specimens as a function of taxonomic designation. We conclude that L. albescens and L. postalba form a single species, with postalba representing a darker‐winged morph along an apparent south‐to‐north wing colour cline. Accordingly, L. postalba is relegated to synonymy under L. albescens (syn.n.).

show abstract

“…Manuscript to be reviewed the economic importance, our understanding of D. stuposa biology or phylogenetic status at the molecular level is still in its infancy. New molecular techniques such as DNA barcoding and PCR-RFLP are considered more reliable than morphology for studying taxonomy of animals (Arimoto & Iwaizum, 2014;Raupach et al, 2010). The application of molecular techniques to study the sequence of D. stuposa mitogenome will help in its precise identification and classification while contributing to future genetic ecology and evolutionary analyses.…”

Section: Introductionmentioning

confidence: 99%

Peer Review #1 of "The complete mitochondrial genome of Dysgonia stuposa (Lepidoptera: Erebidae) and phylogenetic relationships within Noctuoidea (v0.1)"

2020

View full text Add to dashboard Cite

To determine the Dysgonia stuposa mitochondrial genome (mitogenome) structure and to clarify its phylogenetic position, the entire mitogenome of D. stuposa was sequenced and annotated. The D. stuposa mitogenome is 15,721 bp in size and contains 37 genes (protein-coding genes, transfer RNA genes, ribosomal RNA genes) usually found in lepidopteran mitogenomes. The newly sequenced mitogenome contained some common features reported in other Erebidae species, e.g., an A+T biased nucleotide composition and a non-canonical start codon for cox1 (CGA). Like other insect mitogenomes, the D. stuposa mitogenome had a conserved sequen ce 'ATACTAA' in an intergenic spacer between trnS2 and nad1, and a motif 'ATAGA' followed by a 20 bp poly-T stretch in the A+T rich region. Phylogenetic analyses supported D. stuposa as part of the Erebidae family and reconfirmed the monophyly of the subfamilies Arctiinae, Catocalinae and Lymantriinae within Erebidae.

show abstract

Identification of Japanese Lymantria species (Lepidoptera: Lymantriidae) based on PCR–RFLP analysis of mitochondrial DNA

Cited by 21 publications

References 14 publications

Characterization of the Complete Mitochondrial Genome of Leucoma salicis (Lepidoptera: Lymantriidae) and Comparison with Other Lepidopteran Insects

Characterization of the Complete Mitochondrial Genome of Leucoma salicis (Lepidoptera: Lymantriidae) and Comparison with Other Lepidopteran Insects

Reassessment of the status of Lymantria albescens and Lymantria postalba (Lepidoptera: Erebidae: Lymantriinae) as distinct ‘Asian gypsy moth’ species, using both mitochondrial and nuclear sequence data

Peer Review #1 of "The complete mitochondrial genome of Dysgonia stuposa (Lepidoptera: Erebidae) and phylogenetic relationships within Noctuoidea (v0.1)"

Contact Info

Product

Resources

About