Identification of key genes associated with polycystic ovary syndrome (PCOS) and ovarian cancer using an integrated bioinformatics analysis

Abstract

Background Accumulating evidence suggests a strong association between polycystic ovary syndrome (PCOS) and ovarian cancer (OC), but the potential molecular mechanism remains unclear. In this study, we identified previously unrecognized genes that are significantly correlated with PCOS and OC via bioinformatics. Materials and methods Multiple bioinformatic analyses, such as differential expression analysis, univariate Cox analysis, functional and pathway enrichment analysis, protein–protein interaction (PPI) network construction, survival analysis, and immune infiltration analysis, were utilized. We further evaluated the effect of OGN on FSHR expression via immunofluorescence. Results TCGA-OC, GSE140082 (for OC) and GSE34526 (for PCOS) datasets were downloaded. Twelve genes, including RNF144B, LPAR3, CRISPLD2, JCHAIN, OR7E14P, IL27RA, PTPRD, STAT1, NR4A1, OGN, GALNT6 and CXCL11, were identified as signature genes. Drug sensitivity analysis showed that OGN might represent a hub gene in the progression of PCOS and OC. Experimental analysis found that OGN could increase FSHR expression, indicating that OGN could regulate the hormonal response in PCOS and OC. Furthermore, correlation analysis indicated that OGN function might be closely related to m6A and ferroptosis. Conclusions Our study identified a 12-gene signature that might be involved in the prognostic significance of OC. Furthermore, the hub gene OGN represent a significant gene involved in OC and PCOS progression by regulating the hormonal response.

Androgen receptor and osteoglycin gene expression predicting prognosis of metastatic prostate cancer

“…However, in recent years, large-scale sequence analysis has reported various genetic abnormalities in prostate cancer, other than AR. Molecular pathways brought about by these mutations are related to malignancy and drug resistance in prostate cancer [30]. Our study suggests that AR and OGN expression may be prognostic predictors of mHSPC.…”

Validation of mitochondrial biomarkers and immune dynamics in polycystic ovary syndrome

“…47 both PCOS and ovarian cancer. 49 Consistently, earlier research emphasized the significance of NR4A1 in the differentiation and survival of Ly6C-monocytes, as well as their maturation from myeloid dendritic precursors. 50,51 In addition, NR4A1 depletion triggers proinflammatory M1 macrophage polarization with heightened TLR signaling.…”

RNA N6-methyladenosine modification in female reproductive biology and pathophysiology

“…In this study, they confirmed that OGN expression in OC patients was positively correlated with the expression of several m 6 A regulators. We speculate that m 6 A can regulate the occurrence of OC and PCOS through a certain signalling pathway through OGN and even participate in the malignant transformation of PCOS [111].…”