Identification of Key Genes Regulating Sorghum Mesocotyl Elongation through Transcriptome Analysis

Abstract

Sorghum with longer mesocotyls is beneficialfor improving its deep tolerance, which is important for the seedling rates. Here, we perform transcriptome analysis between four different sorghum lines, with the aim of identifying the key genes regulating sorghum mesocotyl elongation. According to the mesocotyl length (ML) data, we constructed four comparison groups for the transcriptome analysis and detected 2705 common DEGs. GO and KEGG enrichment analysis showed that the most common category of DEGs were involved in cell wall, microtubule, cell cycle, phytohormone, and energy metabolism-related pathways. In the cell wall biological processes, the expression of SbEXPA9-1, SbEXPA9-2, SbXTH25, SbXTH8-1, and SbXTH27 are increased in the sorghum lines with long ML. In the plant hormone signaling pathway, five auxin-responsive genes and eight cytokinin/zeatin/abscisic acid/salicylic acid-related genes showed a higher expression level in the long ML sorghum lines. In addition, five ERF genes showed a higher expression level in the sorghum lines with long ML, whereas two ERF genes showed a lower expression level in these lines. Furthermore, the expression levels of these genes were further analyzed using real-time PCR (RT-qPCR), which showed similar results. This work identified the candidate gene regulating ML, which may provide additional evidence to understand the regulatory molecular mechanisms of sorghum mesocotyl elongation.

The Mechanism of Exogenous Salicylic Acid and 6-Benzylaminopurine Regulating the Elongation of Maize Mesocotyl

“…In addition, exogenous brassinosteroid (BR) and IAA have a synergistic effect and promote mesocotyl elongation by acidifying and relaxing the epidermal cell wall [ 13 ]. Exogenous ABA [ 14 ], cytokinin (CTK) [ 15 ], ethylene (ETH) [ 16 ], jasmonic acid (JA) [ 17 ] and SA signal-related transcription factors [ 18 ] also play a regulatory role in mesocotyl length. However, the hormone network regulating the length of maize mesocotyls is not well understood.…”

Integrated Volatile Metabolome and Transcriptome Analyses Provide Insights into the Formation of Benzenoid–Phenylpropanoid Aroma Substance Eugenol in the Rosa hybrida ‘Lanxing’ Flowering

“…Transcriptome provides a way for new gene discovery and transcription identification, which acts as a great role in mining genes related to plant secondary metabolite biosynthesis [19]. A great deal of functional genes have been detected by analyzing different tissues transcriptome results in plants, such as Torreya (Torreya grandis), Sorghum (Sorghum bicolor), and Potato (Solanum tuberosum) [20][21][22]. Metabolome focuses on high-throughput identification and quantification analysis metabolites in organism at a precise time point, which provides insight into ongoing intracellular activities regulated by metabolites [23].…”