Identification of key miRNAs and their targets in peripheral blood mononuclear cells of IgA nephropathy using bioinformatics analysis

Liu, Ling; Yang, YaZhen; Yu, Dongrong

doi:10.1097/md.0000000000026495

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…The detected gene modules may be utilized for subsequent analyses, including biological functions and key genes identification. Although some bioinformatic studies have been applied to exploring potential molecular mechanisms and therapeutic targets in IgAN [ 8 – 10 ], few studies focus on the shared and different genes of glomeruli and tubulointerstitium of IgAN.…”

Section: Introductionmentioning

confidence: 99%

Identification and validation of glomerulotubular crosstalk genes mediating IgA nephropathy by integrated bioinformatics

Bai

2022

BMC Nephrol

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Background IgA nephropathy (IgAN), which has been reported as the most prevalent glomerulonephritis globally, is the major contributor to end-stage renal diseases. This bioinformatics study aimed to explore glomerulotubular crosstalk genes and dysregulated pathways relating to the pathogenesis of IgAN. Methods The microarray datasets from the Gene Expression Omnibus (GEO) database were searched. Weighted gene co-expression network analysis (WGCNA) and differentially expressed genes (DEGs) of both glomeruli and tubulointerstitium were conducted individually. The co-expression gene modules of glomeruli and tubulointerstitium were compared via gene function enrichment analysis. Subsequently, the crosstalk co-expression network was constructed via the STRING database and key genes were mined from the crosstalk network. Finally, key genes were validated using another GEO dataset (GSE99340) containing RNA-seq data of IgAN and lupus nephritis, and their potential diagnostic values were shown using receiver operating characteristic (ROC) analysis. Results Five hundred eighty-three DEGs and eight modules were identified in glomerular samples, while 272 DEGs and four modules were in tubulointerstitial samples. There were 119 overlapping DEGs between the two groups. Among the distinctive modules, four modules in glomeruli and one module in tubulointerstitium were positively associated with IgAN. While four modules in glomeruli and two modules in tubulointerstitium were negatively associated with IgAN. The top ten key genes screened by CytoHubba were ITGAM, ALB, TYROBP, ITGB2, CYBB, HCK, CSF1R, LAPTM5, FN1, and CTSS. Compared with lupus nephritis, there were significant differences in the expression levels of CYBB, CTSS and TYROBP (P < 0.05), while other key genes showed no significant difference. Meanwhile, CYBB, CTSS, and TYROBP demonstrated possible diagnostic significance. Conclusions The crosstalk genes confirmed in this study may provide novel insight into the pathogenesis of IgAN. Immune-related pathways are associated with both glomerular and tubulointerstitial injuries in IgAN. The glomerulotubular crosstalk might perform a role in the pathogenesis of IgAN.

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Section: Introductionmentioning

confidence: 99%

Identification and validation of glomerulotubular crosstalk genes mediating IgA nephropathy by integrated bioinformatics

Bai

2022

BMC Nephrol

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Recent status and trends of innate immunity and the gut-kidney aixs in IgAN: A systematic review and bibliometric analysis

Li,

et al. 2024

International Immunopharmacology

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Magnetic nanoparticle-assisted detection of peripheral blood miRNA biomarker characteristics in syphilis

Zhang

et al. 2022

mat express

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Syphilis is a chronic, systemic, transmitted disease that is highly infectious and harmful. This study was established to analyze the specificity of miRNA in distinguishing patients at different stages of syphilis, so as to provide a theoretical basis for using miRNA as a new diagnostic marker of this disease. The data were collected on patients with secondary syphilis (SS, n = 44) and early latent syphilis (ELS, n = 35) at Shanghai Skin Disease Hospital from March 2017 to March 2021, along with 46 healthy volunteers during the same period as healthy controls. We screened out miRNAs that might be differentially expressed in patients at different stages of syphilis on high-throughput sequencing technology, and then used magnetic nanoparticle-assisted qRT-PCR to verify their expression in the three groups. Receiver operating characteristic (ROC) curve analysis showed that miR-125a-3p could be used to distinguish the normal population from patients with syphilis. Meanwhile, miR-185-3p and miR-660-3p could be used to distinguish patients with ELS and SS, respectively. The combined diagnostic sensitivity of miR-185-3p and miR-660-3p was higher than that of each of these alone. Next, the toluidine red unheated serological test (TRUST) titers in the three groups were determined, with the analysis showing that miR-125a-3p did not have the ability to distinguish patients with different TRUST titers, while miR-185-3p and miR-660-3p levels decreased with increasing TRUST titer, indicating that the levels of miR-185-3p and miR-660-3p also had good diagnostic ability of TP activity. In conclusion, miRNA is differentially expressed in patients with syphilis at different stages of the disease and has good potential as a biomarker of syphilis. The combined use of miRNA increases the sensitivity for diagnosing syphilis at different stages.

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Identification of key miRNAs and their targets in peripheral blood mononuclear cells of IgA nephropathy using bioinformatics analysis

Cited by 3 publications

References 23 publications

Identification and validation of glomerulotubular crosstalk genes mediating IgA nephropathy by integrated bioinformatics

Identification and validation of glomerulotubular crosstalk genes mediating IgA nephropathy by integrated bioinformatics

Recent status and trends of innate immunity and the gut-kidney aixs in IgAN: A systematic review and bibliometric analysis

Magnetic nanoparticle-assisted detection of peripheral blood miRNA biomarker characteristics in syphilis

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