Identification of Key Molecules and lncRNA-miRNA-mRNA ceRNA Network in Preeclampsia

Luo, Shu; Wang, Lina; Li, Shu-Ming; Wang, Hongwei; Huang, Sujing; Zhang, Zhongxia; Wang, Ru; Guan, Hongqiong; Huang, Yuanhua

doi:10.2147/ijgm.s305337

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…Additionally, the ceRNA network was altered in the placentas of PE patients. For example, Luo et al [31] illustrated the differential expression of 726 lncRNAs, 49 miRNAs, and 318 mRNAs in the placentas of PE patients, while Chen et al [32] showed differentially expressed 207 lncRNAs and 33 mRNAs in PE from umbilical vein endothelial cells. This evidence shows that the expression pattern of RNAs profiles was significantly changed in trophoblast cells under hypoxia, which might be involved in the pathophysiology of PE.…”

Section: Discussionmentioning

confidence: 99%

Identification of LncRNA-miRNA-mRNA ceRNA network in hypoxia-induced HTR-8/SVneo cells for preeclampsia

Liu

Wang

et al. 2023

Medicine

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Preeclampsia (PE) is a common pregnancy complication, and placental hypoxia is one of its causes. We aimed to identify the transcriptional profile and construct a long non-coding RNAs (lncRNA)-centered competing endogenous RNAs (ceRNA) network in hypoxia-induced HTR8/SVneo cells. We used datasets from the GEO database to identify important pathways in PE. We performed microarray profiling and functional analysis to identify differentially expressed long non-coding RNAs (lncRNAs), differentially expressed profiles of microRNA (miRNAs), and differentially expressed profiles of messenger RNA (mRNAs) in hypoxia-induced HTR8/SVneo cells. The candidates were validated using quantitative reverse transcription polymerase chain reaction. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes analyses were performed to understand the functional significance of differentially expressed genes. Finally, we constructed an lncRNA-centered ceRNA network. Several hub genes were validated both in placentas from PE and normal pregnancy, and in hypoxia-induced HTR8/SVneo cells. The hypoxic response pathway was involved in the pathophysiology of PE. Subsequently, we identified 536 differentially expressed profiles of lncRNAs (183 upregulated and 353 downregulated), 46 differentially expressed profiles of miRNAs (35 upregulated and 11 downregulated), and 2782 differentially expressed profiles of mRNAs (DEmRNAs) (1031 upregulated and 1751 downregulated) in hypoxia-induced HTR8/SVneo cells. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed potential pathways affected by these genes, such as angiogenesis, the HIF-1 signaling pathway, and the PI3K-Akt signaling pathway. The ceRNA network comprised 35 lncRNAs, 11 miRNAs, 27 mRNAs, and 2 hub lncRNAs, which might play a vital role in placental functions and PE. Our results revealed the transcriptome profile and constructed an lncRNA-centered ceRNA network in hypoxiainduced HTR8/SVneo cells, thereby providing potential therapeutic targets for PE.Abbreviations: BP = biological process, ceRNAs = competing endogenous RNAs, CTBs = cytotrophoblast cells, DEmiRNAs = differentially expressed profiles of miRNAs, DEmRNAs = differentially expressed profiles of mRNAs, EVT = extravillous trophoblast, GO = gene ontology, KEGG = Kyoto Encyclopedia of Genes and Genomes, lncRNAs = long non-coding RNAs, MEF2 = myocyte enhancer factor-2, MEG3 = lncRNA maternally expressed gene 3, messenger RNA = mRNA, microRNA = miRNA, PE = preeclampsia, qRT-PCR = quantitative reverse transcription polymerase chain reaction, SNHG16 = small nucleolar RNA host gene 16.

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Section: Discussionmentioning

confidence: 99%

Identification of LncRNA-miRNA-mRNA ceRNA network in hypoxia-induced HTR-8/SVneo cells for preeclampsia

Liu

Wang

et al. 2023

Medicine

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“…It has been reported that some dysregulated lncRNAs are involved in PE progression. Luo et al [18] reported 726 DElncRNAs and 318 DEmRNAs in placentas from PE patients. Zhang et al [19] found 246 DElncRNAs and 224 DEmRNAs in EOPE samples.…”

Section: Discussionmentioning

confidence: 99%

Identification of lncRNA-miRNA-mRNA networks in late-onset pre-eclampsia

Yao¹,

Liu²,

Liu³

et al. 2022

Reproductive and Developmental Medicine

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Objective: Long non-coding RNAs (lncRNAs) are implicated in multiple pathophysiological processes in placenta-related disorders; however, their expression and function in late-onset pre-eclampsia (LOPE) remain unclear. This study aimed to investigate the expression of lncRNAs in LOPE, construct a competing endogenous RNA (ceRNA) network, and identify the pathways associated with LOPE pathogenesis. Methods: We performed lncRNA and mRNAs microarray profiling to identify the differential expression profiles of lncRNAs and mRNAs in LOPE compared to those in normal pregnancy. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to validate differentially expressed genes. Subsequently, we generated an interaction network between lncRNAs, (micro-RNAs) miRNAs, and mRNAs based on the Pearson’s correlation coefficient between lncRNAs and mRNAs. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to understand the functional significance of differentially expressed lncRNAs (DElncRNAs) in LOPE. Results: We identified 29 DElncRNAs (25 upregulated and four downregulated) and 212 differentially expressed mRNAs (DEmRNAs; 203 upregulated and nine downregulated) in LOPE placentas. Within them, six lncRNAs and four mRNAs were verified by qRT-PCR. GO and KEGG analyses revealed the potential pathways affected by these mRNAs, such as positive regulation of leukocyte chemotaxis, chemokine signaling pathway, and response to hypoxia. Finally, we constructed a ceRNA network including three DElncRNAs and 124 DEmRNAs, whose competing interactions may be mediated by 17 miRNAs. Two DElncRNAs, ENST00000515376 and ENST00000520544, were found to be hub genes, as they interacted with most miRNAs and mRNAs. ENST00000515376 is most likely related to the metabolic process of arachidonic acid, whereas ENST00000520544 is more likely related to the coagulation system, such as the regulation of blood coagulation and platelet degranulation. Conclusion: Differential expression profile of lncRNAs and the lncRNA-miRNA-mRNA network in LOPE provide potential therapeutic targets for this disease.

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“…Since the competing endogenous RNA (ceRNA) network hypothesis was proposed in 2011, researchers have become increasingly aware of the possible potential application of ceRNA network regulation in diagnosing and treating a wide range of diseases [10]. Based on the findings of these studies on pregnant diseases [11][12][13][14][15], we hypothesize that there may be a broad and complex ceRNA regulatory network that will induce SA when network regulation is disrupted. We anticipate that our viewpoint will be helpful as a reference and will aid future research into the pathogenesis of SA and the search for potential RNA markers for diagnosis and treatment.…”

Section: Introductionmentioning

confidence: 99%

Non-Coding RNAs Regulate Spontaneous Abortion: A Global Network and System Perspective

Gan

Yang

et al. 2022

IJMS

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Spontaneous abortion is a common pregnancy complication that negatively impacts women’s health and commercial pig production. It has been demonstrated that non-coding RNA (ncRNA) is involved in SA by affecting cell proliferation, invasion, apoptosis, epithelial-mesenchymal transformation (EMT), migration, and immune response. Over the last decade, research on ncRNAs in SA has primarily concentrated on micro RNAs (miRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs). In this review, we discuss recent ncRNA studies focused on the function and mechanism of miRNAs, lncRNAs, and circRNAs in regulating SA. Meanwhile, we suggest that a ceRNA regulatory network exists in the onset and development of SA. A deeper understanding of this network will accelerate the process of the quest for potential RNA markers for SA diagnosis and treatment.

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Identification of Key Molecules and lncRNA-miRNA-mRNA ceRNA Network in Preeclampsia

Cited by 6 publications

References 35 publications

Identification of LncRNA-miRNA-mRNA ceRNA network in hypoxia-induced HTR-8/SVneo cells for preeclampsia

Identification of LncRNA-miRNA-mRNA ceRNA network in hypoxia-induced HTR-8/SVneo cells for preeclampsia

Identification of lncRNA-miRNA-mRNA networks in late-onset pre-eclampsia

Non-Coding RNAs Regulate Spontaneous Abortion: A Global Network and System Perspective

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