Identification of Key Regulatory Pathways of Basidiocarp Formation in Pleurotus spp. Using Modeling, Simulation and System Biology Studies

Abstract

Pleurotus (Oyster mushroom) is an important cultivated edible mushroom across the world. It has several therapeutic effects as it contains various useful bio-molecules. The cultivation and crop management of these basidiomycete fungi depends on many extrinsic and intrinsic factors such as substrate composition, growing environment, enzymatic properties, and the genetic makeup, etc. Moreover, for efficient crop production, a comprehensive understanding of the fundamental properties viz. intrinsic–extrinsic factors and genotype-environment interaction analysis is required. The present study explores the basidiocarp formation biology in Pleurotus mushroom using an in silico response to the environmental factors and involvement of the major regulatory genes. The predictive model developed in this study indicates involvement of the key regulatory pathways in the pinhead to fruit body development process. Notably, the major regulatory pathways involved in the conversion of mycelium aggregation to pinhead formation and White Collar protein (PoWC1) binding flavin-chromophore (FAD) to activate respiratory enzymes. Overall, cell differentiation and higher expression of respiratory enzymes are the two important steps for basidiocarp formation. PoWC1 and pofst genes were participate in the structural changes process. Besides this, the PoWC1 gene is also involved in the respiratory requirement, while the OLYA6 gene is the triggering point of fruiting. The findings of the present study could be utilized to understand the detailed mechanism associated with the basidiocarp formation and to cultivate mushrooms at a sustainable level.

Comparative transcriptome analysis on candidate genes associated with fruiting body growth and development in Lyophyllum decastes

“…To meet the demands of growth and metabolism, edible fungi are stimulated to produce hemicellulose, cellulose, and various extracellular hydrolases during their development. These enzymes are essential for the degradation, assimilation, conversion, and utilization of large nutrient molecules ( Lechner & Papinutti, 2006 ; Rani, Kalyani & Prathiba, 2008 ; Barh et al, 2022 ; Guigón-López et al, 2014 ). For example, Volvariella volvacea produces multiple forms of extracellular laccase when grown in specific culture conditions, and some laccase genes may play an important role in the development of fruiting bodies ( Chen et al, 2003 ; Lu et al, 2015 ).…”

Edible and Medicinal Macrofungi

“…were identified through variable methods, such as developing a predictive model, simulation, and system biological analysis methods, in particular using an in silico response to environmental factors and involvement of the major regulatory genes. Overall, cell differentiation and higher expression of respiratory enzymes are the two important steps for basidiocarp formation [ 5 ]. Sanghuangporus baumii is a traditional medicinal fungus that produces pharmacological terpenoids.…”