Identification of Key Residues in Virulent Canine Distemper Virus Hemagglutinin That Control CD150/SLAM-Binding Activity

Zipperle, Ljerka; Langedijk, Johannes P. M.; Örvell, Claes; Vandevelde, M.; Zurbriggen, Andreas; Plattet, Philippe

doi:10.1128/jvi.01077-10

Cited by 40 publications

(57 citation statements)

References 29 publications

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“…The residues within this region of CDV-H from the virulent A75/17 strain were replaced with alanine. Detection by flow cytometry of the C-terminally FLAG tagged H proteins (46) confirmed that all mutants except CDV-H I463A were properly expressed at the cell surface (not shown).…”

Section: Structure-guided Design Of Cdv-h Mutantsmentioning

confidence: 84%

“…While residues interacting with SLAM in the morbillivirus H protein have already been mapped (17,38,40,46), two studies recently documented the identification of MeV-H residues responsible for promoting fusion activity in polarized epithelial cells (16,32). These clustered either near or within a recessed groove created by ␤-propeller blades 4 and 5 on one side of H (Fig.…”

Section: Structure-guided Design Of Cdv-h Mutantsmentioning

confidence: 99%

“…Rescue of rA75/17 red /H-Y539A was performed as mentioned above. SLAM-blind H-bearing cDNA clones were generated in a similar way with the difference that the genes were first amplified by PCR from previously reported SLAM-blind expression vectors (46). These H genes additionally contain a FLAG tag sequence (DYKDDDDK) at their C-terminal regions.…”

Section: Vol 85 2011 Insights Into CDV Multiple-receptor Usage 11243mentioning

confidence: 99%

“…Previously reported SLAM-blind H mutants were next assessed for their abilities to trigger fusion in KeR-expressing cells (we selected two single H mutants, the Y525A and R529A mutants; one triple mutant, the Y525A D526A R529A mutant; and one mutant, HSB, containing six mutated residues: D526A, I527A, S528A, R529A, Y547A, and T548A [46]). Consistent with previous results (46), all mutants were strongly defective in supporting fusion activity through the SLAM molecule, although single mutated proteins (especially the Y525A H mutant) retained minimal fusion activity (Fig.…”

Section: Structure-guided Design Of Cdv-h Mutantsmentioning

confidence: 99%

“…All single (and multiple) substitutions performed in pCI-Hwt eF (derived from CDV strain A75/17 hemagglutinin [46]) and in pCI-HOP eF (derived from CDV strain Onderstepoort hemagglutinin [46]) were carried out using the QuikChange Lightning site-directed mutagenesis kit (Stratagene). The pCI-H-SLAM-blind vectors have been described previously (46).…”

Section: Vol 85 2011 Insights Into CDV Multiple-receptor Usage 11243mentioning

confidence: 99%

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Canine Distemper Virus Infects Canine Keratinocytes and Immune Cells by Using Overlapping and Distinct Regions Located on One Side of the Attachment Protein

Langedijk

Janda

Origgi

et al. 2011

J Virol

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The morbilliviruses measles virus (MeV) and canine distemper virus (CDV) both rely on two surface glycoproteins, the attachment (H) and fusion proteins, to promote fusion activity for viral cell entry. Growing evidence suggests that morbilliviruses infect multiple cell types by binding to distinct host cell surface receptors. Currently, the only known in vivo receptor used by morbilliviruses is CD150/SLAM, a molecule expressed in certain immune cells. Here we investigated the usage of multiple receptors by the highly virulent and demyelinating CDV strain A75/17. We based our study on the assumption that CDV-H may interact with receptors similar to those for MeV, and we conducted systematic alanine-scanning mutagenesis on CDV-H throughout one side of the ␤-propeller documented in MeV-H to contain multiple receptor-binding sites. Functional and biochemical assays performed with SLAM-expressing cells and primary canine epithelial keratinocytes identified 11 residues mutation of which selectively abrogated fusion in keratinocytes. Among these, four were identical to amino acids identified in MeV-H as residues contacting a putative receptor expressed in polarized epithelial cells. Strikingly, when mapped on a CDV-H structural model, all residues clustered in or around a recessed groove located on one side of CDV-H. In contrast, reported CDV-H mutants with SLAM-dependent fusion deficiencies were characterized by additional impairments to the promotion of fusion in keratinocytes. Furthermore, upon transfer of residues that selectively impaired fusion induction in keratinocytes into the CDV-H of the vaccine strain, fusion remained largely unaltered. Taken together, our results suggest that a restricted region on one side of CDV-H contains distinct and overlapping sites that control functional interaction with multiple receptors.

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Section: Structure-guided Design Of Cdv-h Mutantsmentioning

confidence: 84%

Section: Structure-guided Design Of Cdv-h Mutantsmentioning

confidence: 99%

Section: Vol 85 2011 Insights Into CDV Multiple-receptor Usage 11243mentioning

confidence: 99%

Section: Structure-guided Design Of Cdv-h Mutantsmentioning

confidence: 99%

Section: Vol 85 2011 Insights Into CDV Multiple-receptor Usage 11243mentioning

confidence: 99%

See 3 more Smart Citations

Canine Distemper Virus Infects Canine Keratinocytes and Immune Cells by Using Overlapping and Distinct Regions Located on One Side of the Attachment Protein

Langedijk

Janda

Origgi

et al. 2011

J Virol

Self Cite

View full text Add to dashboard Cite

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Full genome sequence of a peste des petits ruminants virus (PPRV) from Ghana

et al. 2014

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The full genome of a peste des petits ruminants virus (PPRV) isolated from a sheep lung sample collected in Ghana, Western Africa, in 2010, has been sequenced. Phylogenetic analysis demonstrated that the virus clustered within the lineage II clade while comparison of its full genome with those of other PPRV strains revealed the highest identity (96.6 %) at a nucleotide level with the PPRV strain Nigeria/76/1. This is the first full genome sequence generated for a PPRV lineage II isolated since 1976.

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Canine distemper virus utilizes different receptors to infect chicken embryo fibroblasts and vero cells

2011

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Inducing animal viruses to adapt to chicken embryos or chicken embryo fibroblasts (CEF) is a common method to develop attenuated live vaccines with full security. Canine distemper virus (CDV) also does this, but the mechanisms and particular receptors remain unclear. Virus overlay protein blot assays were carried out on CEF membrane proteins, which were extracted respectively with a Mem-PER™ kit, a radioimmunoprecipitation assay buffer or a modified co-immunoprecipitation method, and revealed a common 57 kDa positive band that differed from the 42-kDa positive band in Vero cells and also from those receptors reported in lymphocytes and 293 cells, indicating a receptor diversity of CDV and the possibility of the 57-kDa protein acting as a receptor that is involved in adaptive infection of CDV Kunming strain to CEF.

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Identification of Key Residues in Virulent Canine Distemper Virus Hemagglutinin That Control CD150/SLAM-Binding Activity

Cited by 40 publications

References 29 publications

Canine Distemper Virus Infects Canine Keratinocytes and Immune Cells by Using Overlapping and Distinct Regions Located on One Side of the Attachment Protein

Canine Distemper Virus Infects Canine Keratinocytes and Immune Cells by Using Overlapping and Distinct Regions Located on One Side of the Attachment Protein

Full genome sequence of a peste des petits ruminants virus (PPRV) from Ghana

Canine distemper virus utilizes different receptors to infect chicken embryo fibroblasts and vero cells

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