Identification of Korean pear cultivars using combinations of SCAR markers

Parentage Confirmation of Korean Bred Pear Cultivars by Simple Sequence Repeat SSR Genotyping and S-Genotypes Analysis

“…The nine Korean bred pear cultivars were collected at the NIHHS (Cho et al 2012; Table 1). SSR genotyping data of 98 pear cultivars were obtained from the literature (Yamamoto et al 2002;2004;Kimura et al 2003;Sawamura et al 2004;Sawamura et al 2008;submitted manuscript).…”

“…Besides those, utilization of self-compatibility phenomenon was an additional breeding objective aimed to develop labor-saving varieties . As a part of that extensive pear breeding efforts throughout the 19th century a total of 27 pear cultivars were released by NIHHS between 1969and 2009(Cho et al 2012. A notable number of varieties including 'Chuwhangbae', 'Whangkeumbae', 'Hanareum', 'Shincheon', 'Soowhangbae', 'Josengwhangkeum', 'Sunhwang' and 'Sooyoung' were bred by using 'Niitaka' as one of the parent in those breeding programs.…”

“…Monte-Corvo et al (2001) reported that inter-simple sequence repeat was effective for identifying parents of P. communis L. cultivars of European origin. Kim et al (2012) analyzed 80 pear cultivars among them 64 were Japanese (P. pyrifolia), 10 were Chinese (P. ussuriensis) and six were European in origin based on their BRIP markers associated DNA profiling. The authors were able to differentiate cultivar 'Housui' from the other Japanese pear cultivars.…”

“…The use of these SSR markers has allowed DNA profiling, parentage analysis, and evaluation of genetic diversity in pear cultivars. To discern the real parent-offspring relationship, some apple cultivars were investigated with S-RNase analysis and SSR markers and revealed their true parentage (Kitahara et al 2005;Moriya et al 2011) In Korea, Cho et al (2012) used 19 sequence-characterized amplified region (SCAR) markers and nine SCAR markers to distinguish 39 pear cultivars (25 Korean pear cultivars and 14 Japanese pear cultivars). Recently, the SNPs, InDels, and SSRs detected between two cultivars 'Whangkeumbae' and 'Minibae' by using NGS data have been mapped to the reference genome of the Chinese pear 'Suli' (P. bretschneideri) (Oh et al 2015).…”

Construction of DNA Profile Data Base of Strawberry Cultivars Using Microsatellite Markers

“…영국에서도 딸기 60개 품종을 microsatellite 마커에 의해 분석한 결과를 보고 한 바 있으며 (Govan et al, 2008), 최근 미국에서는 219개의 딸기 유전자원 및 품종을 대상으로 8개의 microsatellite 마 커를 활용하여 분석한 결과를 보고한 바 있다 (Chambers et al, 2013). 우리나라의 경우는 amplified fragment length polymorphism(AFLP) 및 microsatellite 마커를 이용한 50개 의 딸기 유전자원 및 품종의 유전적 다양성 분석 (Cho et al, 2007), CAPS 마커를 이용한 국내 및 일본에서 육성된 19품 종의 판별 체계 구축에 대한 연구결과가 보고되고 있다 (Cho et al, 2008) (Anderson et al, 1993). …”