Identification of Lactic Acid Bacteria Isolates from Intestine of Milkfish (Chanos-Chanos) Potential Activity against Pathogen Bacteria Used PCR 18s Rrna Methode

Sulistijowati, Rieny; Mile, Lukman

doi:10.14257/ijbsbt.2016.8.3.13

Cited by 2 publications

(2 citation statements)

References 4 publications

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“…The contents of gibberellins was analyzed using HPLC analysis (Barendse et al 1980). Molecular analysis to determine the potential of flowering genes was carried out in several stages: first, DNA extraction from 5 samples of flowering clumps and 5 samples of non-flowering clumps, second, PCR 10 of these bulb samples used one SSR primer (Hd3a), third, electrophoresis from PCR results, fourth, documentation of electrophoresis results using a UVtransluminator (Sulistijowati and Mile 2016).…”

Section: Methodsmentioning

confidence: 99%

Detection of Flowering Ability on Several Bulbs Shallot Sources by using Hd3a and Endogenous GA3 Analysis

Triharyanto

Purnomo

Yunus

et al. 2020

IJARe

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Background: Problems shallot cultivation in tropical regions such as Indonesia, is low productivity. Planting material is one of the causes of low productivity. The planting material used is a bulb that has been used continuously. It is makes planting material susceptible to infectious diseases and viruses. Efforts can be made to reducing the attack of virus infections in the planting material of the bulbs is by using seeds. However, seed production in Indonesia is still low due to the difficulty of flowering and low viability of seeds. The aim of this research was to detect flowering ability from the source of bulbs flowering and non-flowering clumps using Hd3a primers and endogenous GA3, also the effect of age of bulbs to flowering of shallot. Methods: The study used a complete randomized block design. The treatment consisted of two factors with three replications. The first factor was source of bulbs flowering and non-flowering clumps. The second factor was age bulbs which consists of three levels, namely the 60, 80 and 90 days age bulbs. The contents of gibberellins was analyzed using HPLC analysis. Molecular analysis to determine the potential of flowering genes used SSR primer (Hd3a). Result: Hd3a primer can be used as a marker of flowering shallot. Source of bulbs flowering and non-flowering clumps have the same flowering potential gene. The percentage of flowering in flowering clumps was higher than non-flowering clumps and correlates with endogenous gibberellins content. The age of bulbs indicates that older bulbs gave a higher percentage of flowering.

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Section: Methodsmentioning

confidence: 99%

Detection of Flowering Ability on Several Bulbs Shallot Sources by using Hd3a and Endogenous GA3 Analysis

Triharyanto

Purnomo

Yunus

et al. 2020

IJARe

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“…Ikan Bandeng (Chanos chanos) merupakan ikan bernilai ekonomis penting yang telah banyak dibudidayakan diberbagai negara seperti Indonesia (Sulistijowati andMile 2016), Filipina (Santander-de Leon et al 2015), Taiwan (Chiang et al 2004), India (Lalramchhani et al 2019), Srilangka (Vasava et al 2018), Kenya (Mirera, 2019), Tanzania (Mwangamilo dan Jiddawi, 2003), dan Kepulauan Solomon (Blythe et al 2017). Ikan Bandeng banyak dikonsumsi karena mempunyai nilai gizi yang tinggi (Malle et al 2019).…”

Section: Pendahuluanunclassified

Histological Observation Of Milkfish Organs With Stunting Phenomenon

Aris

Malan

2021

JIP

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Milkfish (Chanos chanos) is an important economic fish. Milkfish is widely consumed because it has high nutritional value. Milkfish is also used as live bait for fishing. In the milkfish cultivation system, increasing production is a very important factor. One of the efforts to increase production in fish farming systems is the application of high stocking density. Constraints to the application of the high stocking density cause the growth of milkfish to be non-uniform, some of them even experience stunting, which is a condition in which the fish experience slow growth. This study aims to observe the condition of the milkfish tissue with normal growth and stunted fish. Milkfish (Chanos chanos) used are fish that have been reared for 6 months. Fish organs that were sampled for observation were stunted and normal (non-stunting) fish. The organs observed were the gills, muscles, and intestines. The results showed that stunted milkfish (Chanos chanos) affected the condition of the gill tissue, muscles, and intestines. The gills are edema and necrosis. Muscles undergo edema, degeneration of muscle fibers, and necrosis. The intestine experiences necrosis or cell death.Keywords: Milkfish; Stunting; Histology.AbstrakIkan Bandeng (Chanos chanos) merupakan ikan bernilai ekonomis penting. Ikan Bandeng banyak dikonsumsi karena mempunyai nilai gizi yang tinggi. Ikan bandeng juga dimanfaatkan sebagai umpan hidup untuk penangkapan ikan. Pada sistem budidaya ikan Bandeng peningkatan produksi menjadi faktor yang sangat penting. Salah satu upaya peningkatan produksi pada sistem budidaya ikan adalah dengan aplikasi padat tebar yang tinggi. Kendala penerapan padat penebaran yang tinggi menyebabkan pertumbuhan ikan Bandeng tidak seragam, bahkan beberapa diantaranya mengalami stunting yang merupakan suatu kondisi dimana ikan mengalami lambatnya pertumbuhan. Penelitian ini, bertujuan untuk mengamati kondisi jaringan ikan bandeng dengan pertumbuhan normal dan ikan yang mengalami stunting. Ikan Bandeng (Chanos chanos) yang digunakan adalah ikan yang telah dipelihara selama 6 bulan. Organ ikan yang menjadi sampel pengamatan adalah ikan yang mengalami stunting dan normal (non-stunting). Organ yang diamati adalah insang, otot, dan usus. Hasil penelitian menunjukan ikan Bandeng (Chanos chanos) yang mengalami stunting mempengaruhi kondisi jaringan insang, otot dan usus. Insang mengalami edema dan necrosis. Otot mengamali edema, degenerasi serabut otot, dan necrosis. Usus mengalami necrosis atau kematian sel.Kata kunci: Ikan Bandeng, Stunting, Histologi

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Identification of Lactic Acid Bacteria Isolates from Intestine of Milkfish (Chanos-Chanos) Potential Activity against Pathogen Bacteria Used PCR 18s Rrna Methode

Cited by 2 publications

References 4 publications

Detection of Flowering Ability on Several Bulbs Shallot Sources by using Hd3a and Endogenous GA3 Analysis

Detection of Flowering Ability on Several Bulbs Shallot Sources by using Hd3a and Endogenous GA3 Analysis

Histological Observation Of Milkfish Organs With Stunting Phenomenon

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