Identification of laticifer-specific genes and their promoter regions from a natural rubber producing plant Hevea brasiliensis

Semi-quantitative RT-PCR analysis of transcripts encoding protease inhibitor inHevea brasiliensisMuell. Arg latex

“…Inside latex, various types of protein have been detected in which they have associated functions in plant defense against pathogens and wounding responses [8][9][10]. In a matter of fact, twenty-five per cent of the proteins in C-and B-serums are known as antimicrobial agents in Hevea brasiliensis.…”

“…In molecular levels, high numbers of Expressed Sequence Tags (ESTs) encoding protease inhibitors were predicted in laticifers [10]. Taken together, the studies showed that the protease inhibitors were encoded by numerous genes.…”

Purification, characterization, and bioassay of putative protease inhibitors from Hevea brasiliensis latex

“…Various type of proteins have been detected in latex fractions in which they have associated functions in plant defense against pathogens and wounding responses (Martin, 1991;Jacob et al, 1993, Aoki et al, 2014. In matter of fact, twentyfive percent of the proteins in C-and B-serums are known as antimicrobial agents in H. brasiliensis.…”

Transcriptional responses of laticifer-specific genes to phytohormones in a suspension-cultured cell line derived from petioles of <i>Hevea brasiliensis</i>

“…Most of these genes are expressed specifically in laticifers (Aoki et al 2014). No detectable rubber transferase activity in the cultured cell line derived from laticifer-containing tissues suggested that there was very low transcription of natural rubber biosynthesis-related genes, and that the properties of laticiferous cells had been lost during dedifferentiation.…”

“…No detectable rubber transferase activity in the cultured cell line derived from laticifer-containing tissues suggested that there was very low transcription of natural rubber biosynthesis-related genes, and that the properties of laticiferous cells had been lost during dedifferentiation. To test this hypothesis, we compared the transcript levels of four natural rubber biosynthesis-related genes, expressed specifically in laticifers (Aoki et al 2014), between the suspension-cultured cell line and various tissues of H. brasiliensis; leaves, stems, and latex, the cytoplasm of laticifers obtained by tapping of the bark of the tree trunks. The targets selected for these analyses were Rubber Elongation Factor (REF) (Dennis and Light 1989), Small Rubber Particle Protein (SRPP) (Oh et al 1999), Hevea Rubber Transferase 1 (HRT1) and Hevea Rubber Transferase 2 (HRT2) (Asawatreratanakul et al 2003).…”