Identification of LecRLK gene family in Cerasus humilis through genomic-transcriptomic data mining and expression analyses

Abstract

Lectin receptor-like protein kinases (LecRLKs) have been shown to be involved in plants’ responses to various biotic and abiotic stresse factors. Cerasus humilis is an important fruit species widely planted for soil and water conservation in northern China due to its strong tolerance to drought and salinity stresses. In this study, a total of 170 LecRLK family genes (125 G-types, 43 L-types and 2 C-types) were identified in the newly released whole-genome sequences of C. humilis. Furthermore, nine representative LecRLK genes in young plants of C. humilis under varying drought and salinity stresses were selected for qRT-PCR analysis. Our systematic comparative analyses revealed the active participation of these nine LecRLK genes in the salt and drought stress responses of C. humilis. The results from our study have provided a solid foundation for future functional verification of these LecRLK family genes and will likely help facilitate the more rapid and effective development of new stress resistant Cerasus humilis cultivars.

Genome-Wide Comprehensive Identification and In Silico Characterization of Lectin Receptor-Like Kinase Gene Family in Barley (Hordeum vulgare L.)

“…Similar motif patterns have been found in CslecRLKs of cucumber ( C. sativus ) and Cerasus humilis showing distinct motif features related to the variations in their protein sequences. In total, 10 conserved motifs were observed in CslecRLKs ranging from 4 to 10 in each protein and 14 conserved motifs in Cerasus humilis [ 84 , 85 ]. Motifs 1 to 5 were predominantly identified in L-type CsLecRLK, whereas motif 1, motif 2, motif 6, and motif 8 were frequently observed in G-type CsLecRLK protein [ 84 ].…”

“…Additionally, the largest number of GmlecRLks was located on chromosome 6, chromosome 12, and chromosome 13 [ 34 ]. Furthermore, C hLecRLK genes of C. humilis were found to be unevenly distributed through eight chromosomes consisting of the majority of ChLecRLK genes (56) on chromosome 3 and lowest on chromosome 8 (3) [ 85 ].…”

Bactericidal and plant defense elicitation activities of Eucalyptus oil decrease the severity of infections by Xylella fastidiosa on almond plants

“…In addition, glutamate receptor also responds to an attack from pathogens (and wounding) by mediating PTI by acting as Ca 2+ channels ( Forde and Roberts, 2014 ). LecRLK has been also described as a positive regulator of plant tolerance to salt stress ( Sun et al., 2013 ) and plays crucial roles in plant development and responses to biotic and abiotic stresses ( Han et al., 2021 ). In case of LecRLK overexpression, it has been reported that pathogen infection due to P. syringae DC3000 activates not only the transcription of LecRLK in Arabidopsis , but also plants overexpressing LecRLK show not only resistant phenotype to P. syringae DC3000 but also reactive oxygen species (ROS)-production and SA accumulation ( Luo et al., 2017 ).…”

Temporal Changes in Flavonoid Components, Free Radical Scavenging Activities and Metabolism-Related Gene Expressions during Fruit Development in Chinese Dwarf Cherry (Prunus humilis)

“…Primer sequences were designed using Premier 5.0 software (PREMIER Bio-soft International, Palo Alto, CA, USA) (Table 1). Quantitative real-time PCR was performed following the method described by Han et al [25]. The relative expression of each gene was calculated by the 2 −∆∆Ct method [26].…”