Identification of Leishmania donovani amastigotes in canine tissues by immunoperoxidase staining

Ferrer, L.; Rabanal, Rosa M.; Domingo, Mariano Cuesta; Ramos, Jonathan Hernández; Fondevila, Dolors

doi:10.1016/s0034-5288(18)30838-5

Cited by 76 publications

(67 citation statements)

References 4 publications

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“…These findings strongly support the suggestion of other authors that the best diagnostic method in screening dogs for evidence of visceral leishmaniasis, in both symptomatic and asymptomatic animals, is the staining of lymph node tissue by immunohistochemical staining 13,20,29 . SANCHEZ et al 28 studied the parasite density in Giemsa-stained smears of mesenteric lymph node and concluded that it was similar in both symptomatic and asymptomatic dogs.…”

Section: Discussionsupporting

confidence: 91%

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Lima

Carneiro

Campos

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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Canine visceral leishmaniasis (CVL) is recognizable by characteristic signs of disease and is highly lethal. The infection, however, may be quite inapparent in some seropositive dogs, and this has raised the polemic question as to whether or not such animals can be a source of infection for Lutzomyia longipalpis, the vector of American visceral leishmaniasis (AVL). In this study we have examined 51 dogs with acute CVL from an AVL area in Pará State, northern Brazil, and compared the parasite density, amastigotes of Leishmania (L.) infantum chagasi, in the skin, lymph node and viscera of symptomatic with that of nine asymptomatic but seropositive dogs (IFAT-IgG). Post-mortem biopsy fragments of these tissues were processed by immunohistochemistry, using a polyclonal antibody against Leishmania sp. The X² and Mann Whitney tests were used to evaluate the means of infected macrophage density (p < 0.05). There was no difference (p > 0.05) in the skin (10.7/mm² x 15.5/mm²) and lymph node (6.3/mm² x 8.3/mm²), between asymptomatic and symptomatic dogs, respectively. It was higher (p < 0.05), however, in the viscera of symptomatic (5.3/mm²) than it was in asymptomatic (1.4/mm²) dogs. These results strongly suggest that asymptomatic or symptomatic L. (L.) i. chagasi-infected dogs can serve as a source of infection, principally considering the highest (p < 0.05) parasite density from skin (10.7/mm² x 15.5/mm²), the place where the vetor L. longipalpis takes its blood meal, compared with those from lymph node (6.3/mm² x 8.3/mm²) and viscera (1.4/mm²x 5.3/mm²).

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Section: Discussionsupporting

confidence: 91%

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Lima

Carneiro

Campos

et al. 2010

Rev. Inst. Med. trop. S. Paulo

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“…Therefore, when serological analysis is not available, immunohistochemical techniques may help in order to confirm the diagnosis. These methods are simple to perform, require no sophisticated equipment and allow unequivocal histopathological diagnosis of leishmaniosis (Ferrer et al, 1998;Bourdoiseau et al, 1997). In this case the pathogenesis of the skin lesion remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Leishmaniosis due toLeishmania infantumin a FIV and FeIV positive cat with a squamous cell carcinoma diagnosed with histological, serological and isoenzymatic methods

Grevot

Hugues²,

Marty³

et al. 2005

Parasite

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“…A maioria dos cães pertencia ao grupo oligossintomático, ao contrário dos resultados de Moreira et al (2007) (Figuras 2b e 3b) contribuiu para concluir o diagnóstico, quer seja positivo ou negativo, com ausência de suspeitos, e isto se deveu à coloração específica das formas amastigotas presentes nos tecidos esplênicos. Esses resultados estão de acordo com vários autores que relataram as técnicas parasitológicas como bastante específicas, mas com a imunoistoquímica oferecendo a vantagem adicional, pelo maior grau de contraste entre os parasitas e as células hospedeiras aumentando a sua sensibilidade e a sua especificidade (FERRER et al, 1988;TAFURI et al, 2004 …”

Section: Positividade Dos Cães Segundo Os Sinais Clínicosunclassified

“…Além disso, existem, atualmente, as técnicas imunoistoquímicas que oferecem a vantagem de aumentar a sensibilidade e a especificidade das técnicas parasitológicas por apresentarem facilidades na execução e alto grau de contraste entre os parasitas e as células hospedeiras (FERRER et al, 1988;TAFURI et al, 2004).…”

Section: Introductionunclassified

Exames parasitológicos, imunoistoquímicos e histopatológicos para detecÇão de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral

Tasca¹,

Buzetti²,

Tenório³

et al. 2009

RBPV

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ResumoO propósito do presente trabalho foi o estudo da Leishmaniose Visceral Canina -LVC por meio de métodos parasitológicos e imunoistoquímicos para a detecção de formas amastigotas de Leishmania (L.) chagasi em baço, além de descrever a histopatologia das lesões esplênicas em 34 cães, com diferentes manifestações clínicas da LVC, eutanasiados pelo Centro de Controle de Zoonoses de Ilha Solteira, SP. Esses animais foram examinados clinicamente antes da eutánásia e de acordo com os sinais clínicos da LVC, foram classificados em três grupos: assintomáticos (8 cães), oligossintomáticos (17 cães) e sintomáticos (9 cães). Após a realização desses exames, dos 34 cães, 22 (64,7%) estavam positivos e 12 (35,3%) negativos. Desses cães positivos, 1/22 (4,5%) era assintomático, 12/22 (54,5%) eram oligossintomáticos e 9/22 (40,1%) sintomáticos. Pela histopatologia, os cães, especialmente os sintomáticos apresentavam o baço com inflamação crônica e espessamento na região capsular e trabecular, além de extensa alteração morfológica na polpa vermelha e branca pela presença de grande quantidade de macrófagos repletos de amastigotas, pela reação granulomatosa inflamatória e pelas áreas hemorrágicas. Os exames histopatológicos e a detecção microscópica direta da L. (L.) chagasi revelaram que o baço é um órgão útil para auxiliar no diagnóstico da LVC. A coloração imunoistoquímica foi a que detectou o maior número de tecidos esplênicos positivos com amastigotas, além de elucidar os casos suspeitos pelos exames parasitológicos, principalmente, nos animais assintomáticos ou oligossintomáticos.Palavras-chave: Leishmania (L.) chagasi, Leishmaniose Visceral, cães, baço, imunoistoquímica. AbstractThe purpose of this work was a Canine Visceral Leishmaniasis -CVL study by parasitological direct examination of Leishmania (L.) chagasi (imprinting and histological), immunohistochemical test and histopathological analysis using spleen tissues from 34 dogs euthanized by the Zoonotic Disease Control Centre from Ilha Solteira, SP, Brazil. According to the clinical signs, the dogs were divided in three groups: asymptomatics (8 dogs), oligosymptomatics (17 dogs) and symptomatics (9 dogs). After the accomplishment of all diagnostic tests, 22 dogs were considered positives (64.7%) and 12 (35.3%) were negatives to CVL. From these positive dogs, 1/22 (4.5%) was asymptomatic, 12/22 (54.5%) were oligosymptomatics and 8/22 (40.1%) were symptomatics. The histopathological study in spleen tissues from positive, especially symptomatic dogs, showed a diffuse chronic inflammation with thickness of capsular and trabecular regions and there was extense morphologic alteration of the red and white pulp by the presence of abundant macrophages full with amastigotes, the granulomatous inflammatory reaction and haemorragic areas. The data of this work from histopathologic examination and direct microcopic visualization of L. (L.) chagasi showed that the spleen was an useful organ to collect sample tissues for CVL diagnosis. The immunostaining detected the highest num...

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Identification of Leishmania donovani amastigotes in canine tissues by immunoperoxidase staining

Cited by 76 publications

References 4 publications

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Canine visceral leishmaniasis due to Leishmania (L.) infantum chagasi in Amazonian Brazil: comparison of the parasite density from the skin, lymph node and visceral tissues between symptomatic and asymptomatic, seropositive dogs

Leishmaniosis due toLeishmania infantumin a FIV and FeIV positive cat with a squamous cell carcinoma diagnosed with histological, serological and isoenzymatic methods

Exames parasitológicos, imunoistoquímicos e histopatológicos para detecÇão de Leishmania chagasi em tecidos esplênicos de cães com leishmaniose visceral

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