IDENTIFICATION OF Leishmania infantum IN PUERTO IGUAZÚ, MISIONES, ARGENTINA

Acosta, Lucrecia; Díaz, Ricardo; Torres, Pedro; Silva, Gustavo; Ramos, María José Rodríguez; Fattore, Gladys; Deschutter, Enrique Jorge; Bornay-Llinares, Fernando J.

doi:10.1590/s0036-46652015000200013

Cited by 18 publications

(13 citation statements)

References 7 publications

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“…On the Argentina border of Foz do Iguaçu, in the city of Puerto Iguazú, the presence of the vector Lu. longipalpis was verified in a study conducted in 2010 (SALOMÓN et al, 2011), and in 2013, the DNA presence of L. infantum was confirmed in samples of dogs from this city (ACOSTA et al, 2015).…”

Section: Introductionmentioning

confidence: 81%

“…Environmental degradation, migratory flows and unplanned urban occupation favor the adaptation of vectors in the environment (SILVA et al, 2015). Acosta et al (2015) reported that VL in Puerto Iguazú, Argentina was established due to changes in the urbanization of the vector and the existence of reservoirs (dogs) and vectors (Lu. longipalpis).…”

Section: Discussionmentioning

confidence: 99%

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Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Dias

Soccol

Pasquali

et al. 2018

Rev. Bras. Parasitol. Vet.

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The aim of this study was to investigate the occurrence of anti-Leishmania spp. antibodies in dogs from localities in the city of Foz do Iguaçu, Paraná state, Brazil, on the border with Argentina and Paraguay. Blood samples dogs were collected to perform the following serologic tests: immunochromatographic DPP® rapid test, indirect immunoenzymatic assay (ELISA) and indirect immunofluorescence assay (IFA). In 2012, 285 dogs were analyzed on Argentina border, and in 2013, serum samples from 396 dogs on the border of Paraguay were collected. Using ELISA for screening and IFA for the confirmatory test, the results showed that the antibody prevalence was 1.8% (5/285) on the border of Argentina and 3.0% (12/396) on Paraguay border. When using the DPP® for screening and ELISA as a confirmatory analysis, we observed a seroreagent prevalence in dogs of 2.5% (7/285) on Argentina border and 5.1% (20/396) on Paraguay border. The non-public collection of domestic waste (p= 0.0004) was shown to be associated with leishmaniasis. This study shows the presence of leishmaniasis and suggest the emergence of canine visceral leishmaniasis in state of Paraná due to the confirmed occurrence of seroreactive dogs on Argentina and Paraguay border, which has environmental and geographical characteristics that favor the spread of the parasite.

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Section: Introductionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Dias

Soccol

Pasquali

et al. 2018

Rev. Bras. Parasitol. Vet.

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“…The first autochthonous case of HVL in an Argentinean city concomitant with CVL cases were reported in 2006, also on the border with Paraguay, but 300 km away from the former focus [11]. In 2010, CVL vector and cases were found in Puerto Iguazú on the three-country border with Paraguay and Brazil, 300 km from the other two foci, as in an apex of an equilateral triangle [12], and CVL and HVL were reported since 2013–2014 [13]. Cases of HVL in the Paraguayan side of the border were known previously since 2008 [14], and the vector in the Brazilian side appeared in 2012 [15].…”

Section: Introductionmentioning

confidence: 99%

Twice upon a time: The progression of canine visceral leishmaniasis in an Argentinean city

et al. 2019

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Canine Visceral Leishmaniasis (CVL) prevalence, spatial distribution and associated factors were assessed in four locations in Iguazú department in 2014 and in Puerto Iguazú city again in 2018. The city areas were divided into a grid of 400x400m cells. All cells were sampled in 2014 and a random subsampling was developed in 2018. In each cell, five dogs clustered in a ‘critical scenario’ (prone to have vectors) were sampled. A rapid immunochromatographic dipstick was used to detect antibodies against Leishmania infantum , confirming by lymph node smears observation and PCR. For Puerto Iguazú, Generalized Linear Models (GLMs) were constructed considering environmental, dog and clinical variables. Pearson's Chi square and Fisher's exact tests were employed to evaluate the association between CVL, dog clinical signs and infestation with other parasites. Cartographic outputs were made and Moran's I indices were calculated as spatial autocorrelation indicators. CVL prevalence rates were 26.18% in 2014 and 17.50% in 2018. No associations were established in environmental models, but dog age and repellent use were significant when running 2014 dog models. Clinical models showed significant associations between seropositive dogs and ophthalmological, dermal signs and onychogryphosis in 2014. In 2018, only adenomegaly was associated. The results of global Moran´s I were not significant but regarding local analysis, six sites in 2014 and one in 2018 presented autocorrelation with neighboring sites. The decrease in CVL prevalence may be associated to transmission stabilization, which could explain the lack of associations with dog-related variables. Further, spatial distribution of CVL is a poor evidence for design of transmission control measures but could be important in case of intensive parasite circulation or when the first autochthonous cases appear. For control success, sensitivity of diagnostic methods, political will and adequate material resources remain critical. Modeling of multiple variables will be required to identify factors that drive disease stabilization/destabilization.

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“…A mucocutaneous (MCL) form of the disease has also been described, which develops as a late reaction to previously untreated cutaneous lesions and may affect nasal cavities, the septum and the palate [ 2 , 3 ]. Urbanization of the leishmaniases has been recorded, particularly regarding the visceral form caused by Leishmania infantum [ 4 , 5 , 6 ] as well as variation in species’ spatial distribution and vector-parasite interactions [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Diversity patterns, Leishmania DNA detection, and bloodmeal identification of Phlebotominae sand flies in villages in northern Colombia

et al. 2018

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Leishmaniases are neglected tropical diseases exhibiting complex transmission cycles due to the number of parasite species circulating, sand fly species acting as vectors and infected mammals, including humans, which are defined in the New World as accidental hosts. However, current transmission scenarios are changing, and the disease is no longer exclusively related to forested areas but urban transmission foci occur, involving some species of domestic animals as suspected reservoirs. The aim of this study was to determine the transmission cycles in urban environments by evaluating sand fly diversity, detection of Leishmania DNA, and bloodmeal sources through intra and peridomestic collections. The study was carried out in Colombia, in 13 municipalities of Cordoba department, implementing a methodology that could be further used for the evaluation of vector-borne diseases in villages or towns. Our sampling design included 24 houses randomly selected in each of 15 villages distributed in 13 municipalities, which were sampled in two seasons in 2015 and 2016. Sand flies were collected using CDC light traps placed in intra and peridomestic habitats. In addition to the morphological identification, molecular identification through DNA barcodes was also performed. A total of 19,743 sand flies were collected and 13,848 of them (10,268 females and 3,580 males) were used in molecular procedures. Circulation of two known parasite species–Leishmania infantum and Leishmania panamensis was confirmed. Blood source analyses showed that sand flies fed on humans, particularly in the case of the known L. infantum vector, P. evansi; further analyses are advised to evaluate the reservoirs involved in parasite transmission. Our sampling design allowed us to evaluate potential transmission cycles on a department scale, by defining suspected vector species, parasite species present in different municipalities and feeding habits.

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IDENTIFICATION OF Leishmania infantum IN PUERTO IGUAZÚ, MISIONES, ARGENTINA

Cited by 18 publications

References 7 publications

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Variables associated with the prevalence of anti-Leishmania spp. antibodies in dogs on the tri-border of Foz do Iguaçu, Paraná, Brazil

Twice upon a time: The progression of canine visceral leishmaniasis in an Argentinean city

Diversity patterns, Leishmania DNA detection, and bloodmeal identification of Phlebotominae sand flies in villages in northern Colombia

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