Identification of Leishmania parasites in clinical samples obtained from cutaneous leishmaniasis patients using PCR-RFLP technique in endemic region, Sanliurfa province, in Turkey

Akkafa, Feridun; Dilmeç, Fuat; Alpua, Zuhre

doi:10.1007/s00436-008-1013-5

Cited by 22 publications

(17 citation statements)

References 14 publications

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“…As shown in other studies, 5,15,21 detection of Leishmania DNA by using PCR is highly sensitive for the diagnosis of CL and identifies species. Specificity of PCR is also high.…”

mentioning

confidence: 52%

Epidemiology of Imported Cutaneous Leishmaniasis at the Hospital for Tropical Diseases, London, United Kingdom: Use of Polymerase Chain Reaction to Identify the Species

Wall¹,

Watson²,

Armstrong³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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This study reviewed all patients diagnosed with imported cutaneous leishmaniasis (CL) at the Hospital for Tropical Diseases in London, United Kingdom, over an 11-year period. Diagnostic and epidemiologic information was collected prospectively for all patients with imported CL to this hospital during 1998–2009. A total of 223 patients were given a diagnosis of CL. Ninety patients were diagnosed with Old World CL, which was caused most commonly by Leishmania donovani complex (n = 20). A total of 71% were tourists to the Mediterranean region, 36% were migrants or visiting friends and relatives, and 17% were soldiers. One hundred thirty-three patients were given a diagnosis of New World CL. The Leishmania subgenus Viannia caused 97 of these cases; 44% of these were in backpackers and 29% were in soldiers. Polymerase chain reaction was more sensitive and faster for detecting Leishmania DNA (86% for Old World CL and 96% for New World CL) than culture. This is the largest study of imported leishmaniasis, and demonstrates that tourists to the Mediterranean and backpackers in Central and South America are at risk for this disease.

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“…As shown in other studies, 5,15,21 detection of Leishmania DNA by using PCR is highly sensitive for the diagnosis of CL and identifies species. Specificity of PCR is also high.…”

mentioning

confidence: 52%

Epidemiology of Imported Cutaneous Leishmaniasis at the Hospital for Tropical Diseases, London, United Kingdom: Use of Polymerase Chain Reaction to Identify the Species

Wall¹,

Watson²,

Armstrong³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…Akkafa et al. 20 reported the polymerase chain reaction results of the causative Leishmania species in 51 patients with CL. In 49 patients, L. tropica was the causative agent, and in two they could not show any organism.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological and clinical characteristics of 7172 patients with cutaneous leishmaniasis in Sanliurfa, between 2001 and 2008

et al. 2012

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“…We selected the conserved region of minicircle kinetoplast DNA because its copy number is more than 10 4 per parasite, maximizing the possibility of detection (Akkafa et al 2008;Smyth et al 1992;Salotra et al 2001). PCR analysis consisted of two steps, the details of which were given previously (Aransay et al 2000;Pandey et al 2008).…”

Section: Pcr Amplificationmentioning

confidence: 99%

Diagnosis of visceral leishmaniasis by polymerase chain reaction of DNA extracted from Giemsa's solution-stained slides

Pandey

Mallik³

et al. 2010

Parasitol Res

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Visceral leishmaniasis (VL) is caused by the protozoan parasite Leishmania donovani and is a potentially fatal disease in endemic areas of the world. Nepal is an endemic area in which VL causes major public health problems in the lowland areas of the southeast regions. The aim of the present study was to evaluate the sensitivity of polymerase chain reaction (PCR) amplification for the detection of Leishmania DNA from Giemsa's solution-stained bone marrow slides. Bone-marrow samples were aspirated from a total of 115 VL suspected patients and used to prepare smears on glass slides and for the initiation of in vitro culture.Bone-marrow slides were used for microscopic observation and for DNA extraction, and subsequent PCR amplification. PCR analysis showed that all the positive samples were of Leishmania parasites. The PCR assay also showed a higher sensitivity (69%) than microscopic examination (57%) and culture (21%). In addition, PCR was able to detect VL in 12% of samples which were negative by microscopy. PCR of DNA extracted from Giemsa's solution-stained bone-marrow slides is a suitable tool for confirming diagnosis in patients with VL and may also be useful in the diagnosis of difficult cases. Bone-marrow smears are easily stored, and can be easily sent to research centers where PCR is available. This makes PCR is good option for diagnosis in the field.

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Identification of Leishmania parasites in clinical samples obtained from cutaneous leishmaniasis patients using PCR-RFLP technique in endemic region, Sanliurfa province, in Turkey

Cited by 22 publications

References 14 publications

Epidemiology of Imported Cutaneous Leishmaniasis at the Hospital for Tropical Diseases, London, United Kingdom: Use of Polymerase Chain Reaction to Identify the Species

Epidemiology of Imported Cutaneous Leishmaniasis at the Hospital for Tropical Diseases, London, United Kingdom: Use of Polymerase Chain Reaction to Identify the Species

Epidemiological and clinical characteristics of 7172 patients with cutaneous leishmaniasis in Sanliurfa, between 2001 and 2008

Diagnosis of visceral leishmaniasis by polymerase chain reaction of DNA extracted from Giemsa's solution-stained slides

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