Identification of lipase producing bacteria from palm oil sewage sludge processing plant at Malimping, Banten, Indonesia

Layly, Ika Rahmatul; Meryandini, Anja; Helianti, Is; Astuti, Rika Indri

doi:10.13057/biodiv/d221045

Cited by 4 publications

(3 citation statements)

References 38 publications

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“…An investigation of the screening of lipase-producing bacteria using tributyrin (TBA) agar media was performed using Chryseobacterium gleum and Bacillus velezensis isolated from oily sludge sewage (18) . The zone of clearance of Pseudomonas chengduensis and Azospirillum zeae when compared to the above species showed a greater zone of clearance around TBA plates, indicating higher lipase activity.…”

Section: Fig 4 Lipase Activity On Tributyrin Agar Plate After 48 H Of...mentioning

confidence: 99%

Enzyme Profiling of Petroleum Hydrocarbon-Degrading Bacteria Isolated from Oil Contaminated Soils

Nanekar,

Kokitkar

2024

IJST

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Objectives: The main objective of this study was to evaluate the activity of enzymes with hydrocarbon-degrading potential. Methods: Four petroleum hydrocarbon-degrading bacterial strains, S4P2, S14P1, S9D2, and S13D1, were isolated and used to screen the activity of catalase, lipases, and catechol 1, 2-dioxygenase. All the strains were characterized biochemically. The presence of catalase was studied using nutrient agar and absorbance was measured at 240 nm due to the disappearance of peroxide. Qualitatively, the presence of lipase was studied on tributyrin agar (TBA) plates. The lipase activity was quantified using the titrimetric method. The activity of catechol 1, 2-dioxygenase (C12O) was screened by the formation of the product cis, cis-muconic acid (CCMA) by measuring the absorbance at 260 nm. Findings: Bacterial strains S4P2, S14P1, S9D2, and S13D1 showed clear halo zones around TBA plates, indicating lipase activity. Similarly, quantitively lipase activity was highest in S4P2 (0.034±0.01 U/ml) and S13D1 (0.027±0.01 U/ml) followed by S9D2 (0.024±0.01 U/ml) and S14P1 (0.024±0.01 U/ml) respectively. The presence of catalase was confirmed by effervescence after the addition of H2O2 on cultured nutrient agar, quantitative screening was performed by checking the decrease in absorbance at 240 nm. The maximum activity was observed in isolates S9D2 and S14P1. The total catechol 1, 2-dioxygenase activity was found to be 18.48±0 U/ml and 8.96±0.56 U/ml in S9D2 and S13D1. In contrast, S14P1 and S4P2 showed 12.88±0.56 U/ml and 16. 8±0.U/ml. Novelty: Microbial enzymes play an important role in the degradation of hydrocarbons. The direct application of microbes offers many constraints, so the use of these enzymes as a cocktail may be a solution for bioremediation. In the present study, we aimed to elucidate the enzymes responsible for hydrocarbon degradation in previously unreported strains, Pseudomonas donghuensis and Azospirillum zeae. Therefore, this enzyme can be used as a bioremediation agent for petroleum-contaminated sites. Keywords: Petroleum; Catalase; Lipases; Catechol 1,2-dioxygenase; Hydrocarbons

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Section: Fig 4 Lipase Activity On Tributyrin Agar Plate After 48 H Of...mentioning

confidence: 99%

Enzyme Profiling of Petroleum Hydrocarbon-Degrading Bacteria Isolated from Oil Contaminated Soils

Nanekar,

Kokitkar

2024

IJST

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“…Tujuan dari purifikasi isolat bakteri yaitu untuk memperoleh koloni tunggal dengan morfologi yang berbeda dengan pemisahan dari koloni lain ataupun kontaminan. Koloni yang dimurnikan ini diseleksi berdasarkan perbedaan morfologi seperti warna, tekstur, elevasi, margin, dan lingkar konsentrisnya (Layly et al, 2021).…”

Section: Karakterisasi Morfologi Koloni Isolat Bakteriunclassified

“…Katalase mampu untuk menetralisir efek berbahaya dari hidrogen peroksida dengan cara menguraikannya menjadi air dan oksigen sehingga tidak bersifat toksik. Selama respirasi, setiap mikroorganisme baik aerobik, anaerobik, maupun fakultatif aerobik selalu memproduksi hidrogen peroksida yang bersifat toksik (Layly et al, 2021).…”

Section: Uji Biokimiaunclassified

Isolasi dan Identifikasi Bakteri Pendegradasi Limbah Cair Minyak Kelapa Sawit (Elaeis guineensis Jacq)

Aida,

Manalu

2023

BIOEDUSAINS

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The purpose of this study was to determine the presence of degrading bacteria in the Simangalam river containing palm oil wastewater in Gunung Melayu Village, North Labuhanbatu Regency. This research was conducted using a survey method on the presence of bacteria and the characteristics of bacterial isolates degrading palm oil industrial wastewater and the data obtained were analyzed descriptively. The results showed that only HB1ST1 and HB2ST2 were circular, while HB3ST2, HB4ST2, HB5ST2, and H62ST2 were irregular. After gram staining, three gram-positive bacterial isolates (HB1ST1, HB4ST2, and HB6ST2) and three gram-negative bacterial isolates (HB2ST2, HB3ST2, and HB5ST2) were obtained. The HB1ST1 isolate motility test showed negative results, while the other five isolates (HB2ST2, HB3ST2, HB4ST2, HB5ST2, and HB6ST2) showed positive results. The conclusion of this study is that there are 6 bacterial isolates that are able to degrade oil palm liquid waste in the Simangalam River from 2 samples of oil palm liquid waste. Keywords: Descriptive, Isolate, Palm Oil Effluent

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Immobilization of an endophytic Bacillus sp. on Phragmites karka stem for lipase production in the presence of Cassia fistula seeds

Ashraf,

Sohail,

Abideen

et al. 2024

Biofuels Bioprod Bioref

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Endophytic bacteria have not been reported widely for their lipolytic abilities, so they are not used for large‐scale lipase production. The purpose of this study was to explore an endophytic bacterium for the production of lipase implementing cost‐effective techniques, including the use of Cassia fistula seeds, as a substrate in the production medium and stem pieces of Phragmites karka as the immobilization matrix. The endophytic strain Bacillus sp. E4 was originally isolated from the halophytic plant Arthrocnemum macrostachyum. Bacillus sp. E4 produced 6.05 IU mL−1 lipase in the presence of powdered seeds of Cassia fistula (golden shower tree). Initial trial experiments using a one‐factor‐at‐a‐time approach led to an improvement in lipase titers to 10.05 IU mL−1. Consequently, investigations using the Plackett–Burman design suggested the influence of three significant factors – incubation period, inoculum size, and substrate concentration – on lipase production. They were optimized using the Box–Behnken design (BBD). In the response optimization experiment, strain E4 yielded 52.35 IU mL−1 lipase, which was in accordance with the predicted yield and indicated an overall 8.65 fold improvement in lipase production. To investigate the use of free cells, strain E4 was immobilized on the stem pieces of a halophytic plant, Phragmites karka, which was used for the first time as an immobilization matrix. The immobilized cells retained lipase production ability for up to six cycles with the highest yield of 110 IU mL−1, which corresponded to an improvement of more than eighteenfold. Scanning electron micrographs confirmed the colonization of E4 cells in the matrix and demonstrated the utilization of C. fistula seeds. Fourier transform infrared spectroscopy affirmed the utilization of components including fatty acids by the immobilized E4 cells. The study suggests that endophytic bacterial strains could be applied for the production of lipase with the utilization of nontraditional oil sources.

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Identification of lipase producing bacteria from palm oil sewage sludge processing plant at Malimping, Banten, Indonesia

Cited by 4 publications

References 38 publications

Enzyme Profiling of Petroleum Hydrocarbon-Degrading Bacteria Isolated from Oil Contaminated Soils

Enzyme Profiling of Petroleum Hydrocarbon-Degrading Bacteria Isolated from Oil Contaminated Soils

Isolasi dan Identifikasi Bakteri Pendegradasi Limbah Cair Minyak Kelapa Sawit (Elaeis guineensis Jacq)

Immobilization of an endophytic Bacillus sp. on Phragmites karka stem for lipase production in the presence of Cassia fistula seeds

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