Identification of Listeria monocytogenesVirulence Factors in Women With Abortion by Polymerase Chain Reaction

Eslami, Gita; Goudarzi, Hossein; Ohadi, Elnaz; Taherpour, Arezou; Taheri, Saied

doi:10.5812/archcid.19931

Cited by 11 publications

(4 citation statements)

References 10 publications

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“…Of 80 vaginal samples, 9 (11.3%) were positive for L. monocytogenes . This rate was lower in comparison to the rate of 16.6% in the study of Eslami et al (21), and 14.2% reported by Pournajaf et al, both from Iran (22). In both latter studies, the reason for the difference is the different geographic locations studied.…”

Section: Discussioncontrasting

confidence: 67%

Isolation and characterization of Listeria monocytogenes from environmental and clinical sources by culture and PCR-RFLP methods

Meghdadi¹,

Khosravi²,

Sheikh³

et al. 2019

IJM

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Background and Objectives: Due to the widespread distribution of Listeria monocytogenes in environmental and animal sources and serious clinical complications in human, this study was aimed to isolate L. monocytogenes from water and clin- ical specimens by culture and PCR methods and to investigate the presence of hlyA and inlA virulence genes. Materials and Methods: Water and clinical samples of vaginal and fecal were screened for the presence of L. monocyto- genes by phenotypic and standard biochemical tests. PCR amplification was performed on extracted DNA using primers based on the hlyA and inlA genes. A 733-bp fragment of inlA gene was used for investigation of polymorphism using RFLP analysis. Results: In total, 45 phenotypically and molecularly confirmed L. monocytogenes strains were isolated from different sourc- es including 30 (16.7%) from water, 9 (11.3%) from vaginal swabs and 6 (7.5%) from fecal samples. RFLP analysis of PCR products using AluI and Tsp509I restriction enzymes, generated two profiles with 8 to 10 bands ranging in size from 15 to 210 bp. The majority of water and clinical isolates were classified in profile 2. Conclusion: We demonstrated 45 L. monocytogenes isolates from tested water and clinical samples by phenotypic and mo- lecular tests. The majority of the isolates were classified in the same RFLP profile, showing the water as a potential source of clinical complications in patients in the region of study.

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Section: Discussioncontrasting

confidence: 67%

Isolation and characterization of Listeria monocytogenes from environmental and clinical sources by culture and PCR-RFLP methods

Meghdadi¹,

Khosravi²,

Sheikh³

et al. 2019

IJM

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“…Eslami and colleagues used the PCR method and reported that about 16% of 96 women with recurrent abortions were infected with L. monocytogenes (18). Goudarzi and colleagues detected seven cases of L. monocytogenes positive (among 87 vaginal swab specimens from women with abortion) using the PCR assay (36).…”

Section: Discussionmentioning

confidence: 99%

“…According to the previous studies, the semen analysis is the most reliable test to screen infertile males (1); the use of PCR is also recommended as it is highly sensitive and specific to C. trachomatis in infertile males and L. monocytogenes in females with recurrent abortion (13,(17)(18)(19). Detection of C. trachomatis in infertile males is associated with various challenges, but PCR can detect the bacteria in eight hours.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Chlamydia trachomatis and Listeria monocytogenes in Infertile Men and the Effect on Semen Parameters

Tohidpour

Shahhosseiny

Mehrabian

et al. 2020

Jundishapur J Microbiol

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Background: Infertility is one of the serious problems in gynecology and one of the most important issues of concern in couples. Meanwhile, a significant rise in infertility is recently reported in Iran due to the infections and harsh environmental conditions. Objectives: The current study aimed to detect Chlamydia trachomatis and Listeria monocytogenes in males with infertility using PCR, and to evaluate bacteriospermia effects of the studied bacteria on semen parameters. Methods: Semen specimens of 100 infertile men were collected. Then, each specimen was divided into two parts: the first part was tested by semen analysis according to the WHO guidelines and the second was tested using the PCR method. The PCR intended to identify C. trachomatis and L. monocytogenes. Results: Out of 100 semen samples, 20% were positive for C. trachomatis, 3% were positive for L. monocytogenes, and 3% were positive for both bacteria (coinfection). The leukocyte count was higher than the normal range (0 - 1 Mil/mL) in all semen specimens. The prevalence of C. trachomatis in azoospermic patients was significantly higher than that of nonazoospermic (P < 0.05). However, there was no significant difference between the two groups in terms of the detection of L. monocytogenes (P > 0.05). Detection of C. trachomatis and L. monocytogenes had no significant association with abnormal semen parameters in asymptomatic patients (P > 0.05). Conclusions: The results indicated that precise analysis of semen parameters and diagnosis of leukocytospermia in patients using the PCR can be considered as a rapid and accurate technique to detect bacteria such as C. trachomatis and L. monocytogenes in semen specimens. Therefore, the utilization of this technique in the screening programs for asymptomatic infertile couples can be helpful for early treatment.

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“…It has been well documented that different proteins, including internalins (encoded by int genes), hemolysin (encoded by hly gene), phosphatidyl inositol (PI-PLC) (encoded by plcA gene), and ActA (coded by actA gene), play a key role in mediating the infection cycle of L. monocytogenes [6][7][8] . L. monocytogenes is able to grow in a wide pH range, tolerate salt, grow at low temperatures, and survive various stress conditions [4,9] . Consumption of readyto-eat (RTE) food, undergoing industrial processings and requiring storage at low temperatures, is often considered as a source of listerial infections [10] .…”

Section: Introductionmentioning

confidence: 99%

Association among Biofilm Formation, Serogroups, and Virulence Factors in Listeria monocytogenes Isolated from Food, Clinical, and Livestock Sources

Parvin

Sorkhabi²,

Ohadi³

et al. 2022

IEM

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Backgrounds: Listeria monocytogenes is an opportunistic pathogen causing listeriosis, its pathogenicity is due to the presence of virulence factors including InlA, InlB, PlcA, PlcB, ActA, Iap, and Hly. The purpose of this study was to evaluate the formation of biofilm and its association with serotypes and virulence factors in L. monocytogenes isolates. Materials & Methods: In this study, 51 L. monocytogenes isolates were collected from blood, urine, feces, placenta, rectum, and vagina samples as well as livestock and food samples. Biofilm production was measured using microtiter plate assay, and virulence genes were identified by PCR method. Findings: Out of 51 isolates, 27 (52.9%) were non-biofilm producers, 17 (33.3%) were weak biofilm producers, four (7.8%) were medium biofilm producers, and three (5.9%) were strong biofilm producers. According to this study results, different L. monocytogenes strains could form biofilm with various intensities. The actA, flaA, inlJ, inlA, and plcB genes were observed in all the isolates. The frequency of the hlyA, plcA, iap, inlB, and inlC genes among the isolates was 90.2, 94.1, 98, 88.2, and 82.4%, respectively. There was no significant correlation between the presence/absence of virulence genes in biofilm producing and non-biofilm forming isolates, except for the inlC and iap genes, which showed a significant correlation with the ability to form biofilm. Conclusions: Due to the high prevalence rate of biofilm formation among the isolates and the importance of biofilm production in medical surfaces and food industries, eradication of biofilm-forming isolates is important.

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Identification of Listeria monocytogenesVirulence Factors in Women With Abortion by Polymerase Chain Reaction

Cited by 11 publications

References 10 publications

Isolation and characterization of Listeria monocytogenes from environmental and clinical sources by culture and PCR-RFLP methods

Isolation and characterization of Listeria monocytogenes from environmental and clinical sources by culture and PCR-RFLP methods

Prevalence of Chlamydia trachomatis and Listeria monocytogenes in Infertile Men and the Effect on Semen Parameters

Association among Biofilm Formation, Serogroups, and Virulence Factors in Listeria monocytogenes Isolated from Food, Clinical, and Livestock Sources

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