Identification of low abundance cyclophilins in human plasma

Schümann, Michael; Ihling, Christian; Prell, Erik; Schierhorn, Angelika; Sinz, Andrea; Fischer, Günter; Schiene‐Fischer, Cordelia; Malešević, Miroslav

doi:10.1002/pmic.201600221

Cited by 9 publications

(3 citation statements)

References 60 publications

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“…Elevated levels of CypB expression have been identified in HCC [ 10 ], breast cancer [ 11 ], pancreatic cancer [ 12 ], gastric cancer [ 13 ], and glioblastoma [ 14 ]. Both CypA and CypB are proposed as potential therapeutic targets and biomarkers for the diagnosis of inflammatory conditions [ 15 ]. Another member of the Cyp family, hCypH, also recognized as Ppih , assists in protein folding by catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

PPIH gene regulation system and its prognostic significance in hepatocellular carcinoma: a comprehensive analysis

Ye,

Pang,

Yang

et al. 2023

Aging

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Background: Peptidyl-prolyl isomerase H (PPIH) is a member of the cyclophilin protein family, which functions as a molecular chaperone and is involved in the splicing of pre-mRNA. According to reports, the malignant progression of HCC related to hepatitis B virus (HBV) is tightly associated with RNA-binding proteins. Nevertheless, there is no research on PPIH expression or its function in the occurrence and progression of HCC. Results: We are the first to reveal that the mRNA and protein levels of Ppih are substantially overexpressed in HCC, as the outcomes show. A significant correlation existed between enriched expression of Ppih within HCC and more advanced, poorly differentiated, and TP53-mutated tumors. Conclusion: These findings, which suggest that Ppih may serve as a predictive biomarker for people with HCC, serve as a starting point for further investigation into the function of Ppih in the progression of carcinogenesis. Methods: Accordingly, we utilized clinical samples and bioinformatics analysis to assess Ppih’s mRNA, protein expression, and gene regulatory system in HCC. Additionally, Wilcoxon signed-rank testing and logistic regression were utilized to inspect the association between clinicopathological factors and Ppih . Clinical pathological traits linked to overall survival (OS) among HCC patients were examined via TCGA data via Cox regression and the Kaplan-Meier approach. Additionally, via TCGA data collection, gene set enrichment assessment was also conducted.

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Section: Introductionmentioning

confidence: 99%

PPIH gene regulation system and its prognostic significance in hepatocellular carcinoma: a comprehensive analysis

Ye,

Pang,

Yang

et al. 2023

Aging

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“…Since the transportation of Cyp40 has not been studied yet 33 , we conducted research on how it transfers between cells. Examination of the co-culture medium indicated that Cyp40 was not present in the exosomes (Fig.…”

Section: Three-pointmentioning

confidence: 99%

Sensory nerves directly promote osteoclastogenesis by secreting Peptidyl-prolyl cis-trans isomerase D (Cyp40)

Pei

Yang

Liu

et al. 2023

Preprint

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Given the afferent functions, sensory nerves recently have been found exerting efferent influences and directly alter organ physiology. Moreover, studies have demonstrated the crucial role of sensory nerves in the physiological function of osteoclasts. However, the direct evidence of whether sensory nerve efferent influences osteoclast, remains lacking. Here, we found abundant efferent signals transported from sensory nerves into osteoclasts; sensory hypersensitivity significantly increased osteoclastic bone resorption; and sensory neurons (SN) directly promote osteoclastogenesis in vitro co-culture system. Then our screening identified a novel neuropeptide Cyp40, is the efferent signal from the sensory nerve and plays a critical role in the osteoclastogenesis, via aryl hydrocarbon receptor (AhR)-Ras/Raf-pErk-NFATc1 pathway. These findings revealed a novel mechanism of sensory nerves on bone regulation: the direct promotion of osteoclastogenesis by secreting Cyp40. Inhibiting the Cyp40 could therefore be a strategy to improve bone quality in osteoporosis and promote bone repair after bone injury.

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“…However, this problem can be tackled at the level of elution. An interesting strategy based on selective elution was recently examined .…”

Section: Monoliths For High‐throughput Protein Purification or Screeningmentioning

confidence: 99%

Use of monolithic supports for high‐throughput protein and peptide separation in proteomics

2017

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The exclusive properties of monolithic supports enable fast mass transfer, high porosity, low back pressure, easy preparation process and miniaturisation, and the availability of different chemistries make them particularly suitable materials for high-throughput (HTP) protein and peptide separation. In this review recent advances in monolith-based chromatographic supports for HTP screening of protein and peptide samples are presented and their application in HTP sample preparation (separation, enrichment, depletion, proteolytic digestion) for HTP proteomics is discussed. Development and applications of different monolithic capillary columns in HTP MS-based bottom-up and top-down proteomics are overviewed. By discussing the chromatographic conditions and the mass spectrometric data acquisition conditions an attempt is made to present currently demonstrated capacities of monolithic capillary columns for HTP identification and quantification of proteins and peptides from complex biological samples by MS-based proteomics. Some recent advances in basic monolith technology of importance for proteomics are also discussed.

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Identification of low abundance cyclophilins in human plasma

Cited by 9 publications

References 60 publications

PPIH gene regulation system and its prognostic significance in hepatocellular carcinoma: a comprehensive analysis

PPIH gene regulation system and its prognostic significance in hepatocellular carcinoma: a comprehensive analysis

Sensory nerves directly promote osteoclastogenesis by secreting Peptidyl-prolyl cis-trans isomerase D (Cyp40)

Use of monolithic supports for high‐throughput protein and peptide separation in proteomics

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