Identification of Low Concentrations of Flucytosine Drug Using a Surface-Enhanced Raman Scattering (SERS)-Active Filter Paper Substrate

Karooby, Elaheh; Sahbafar, Hossein; Heris, Masoud Hakimi; Hadi, Amin; Eskandari, Vahid

doi:10.1007/s11468-023-02042-1

Cited by 17 publications

(2 citation statements)

References 48 publications

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“…The ability to study these parameters, to obtain mechanical characteristics and topographic maps of living cells at the same time, will significantly expand the SICM toolkit. It is possible to obtain data about molecular composition of the cell parts using surface-enhanced Raman scattering (SERS) spectroscopy, whose unique sensitivity is known to provide detection and identification of single molecules [17,18]. In this case, a pipette plays the role of an SERS-active tool [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Single-Cell Analysis with Silver-Coated Pipette by Combined SERS and SICM

Dubkov,

Overchenko,

Novikov

et al. 2023

Cells

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The study of individual cell processes that occur both on their surface and inside is highly interesting for the development of new medical drugs, cytology and cell technologies. This work presents an original technique for fabricating the silver-coated pipette and its use for the cell analysis by combination with surface-enhanced Raman spectroscopy (SERS) and scanning ion-conducting microscopy (SICM). Unlike the majority of other designs, the pipette opening in our case remains uncovered, which is important for SICM. SERS-active Ag nanoparticles on the pipette surface are formed by vacuum–thermal evaporation followed by annealing. An array of nanoparticles had a diameter on the order of 36 nm and spacing of 12 nm. A two-particle model based on Laplace equations is used to calculate a theoretical enhancement factor (EF). The surface morphology of the samples is investigated by scanning electron microscopy while SICM is used to reveal the surface topography, to evaluate Young’s modulus of living cells and to control an injection of the SERS-active pipettes into them. A Raman microscope–spectrometer was used to collect characteristic SERS spectra of cells and cell components. Local Raman spectra were obtained from the cytoplasm and nucleus of the same HEK-293 cancer cell. The EF of the SERS-active pipette was 7 × 105. As a result, we demonstrate utilizing the silver-coated pipette for both the SICM study and the molecular composition analysis of cytoplasm and the nucleus of living cells by SERS. The probe localization in cells is successfully achieved.

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Section: Introductionmentioning

confidence: 99%

Single-Cell Analysis with Silver-Coated Pipette by Combined SERS and SICM

Dubkov,

Overchenko,

Novikov

et al. 2023

Cells

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“…The substrate’s geometry, particularly the surface localization of nanostructures, is crucial for sustaining strong surface plasmon resonance (SPR), and the surface chemistry of these nanostructures significantly influences the intensity of SPR peaks [ 11 , 12 , 13 , 14 , 15 ]. At present, combining semiconductor nanostructures with noble metal nanoparticles, known as metal–semiconductor heterostructures, offers a promising approach to creating three-dimensional (3D) LSPR structures to enhance SERS substrates’ detection sensitivity and uniformity [ 16 , 17 , 18 , 19 , 20 , 21 ]. These heterostructures can serve as recyclable SERS substrates through the photocatalytic degradation of target molecules under UV light irradiation and facilitate charge transfer pathways for Raman scattering enhancement [ 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Na2Ti3O7@RF@Ag Heterostructures as Efficient Substrates for SERS and Photocatalytic Applications

Chang,

Lin,

Chin

et al. 2023

Molecules

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A multi-step procedure was effectively employed to synthesize innovative three-dimensional (3D) heterostructures encompassing sodium titanate (Na2Ti3O7) nanowire cores, an intermediate resorcinol–formaldehyde (RF) layer, and outer silver (Ag) nanoparticle sheaths, referred to as Na2Ti3O7@RF@Ag heterostructures. Initially, a one-step hydrothermal technique facilitated the direct growth of single-crystal Na2Ti3O7 nanowires onto a flexible Ti foil. Subsequently, a two-step wet chemical process facilitated the sequential deposition of an RF layer and Ag nanoparticles onto the Na2Ti3O7 nanowires at a low reaction temperature. Optimal concentrations of silver nitrate and L-ascorbic acid can lead to the cultivation of Na2Ti3O7@RF@Ag heterostructures exhibiting heightened surface-enhanced Raman scattering (SERS), which is particularly beneficial for the detection of rhodamine B (RhB) molecules. This phenomenon can be ascribed to the distinctive geometry of the Na2Ti3O7@RF@Ag heterostructures, which offer an increased number of hot spots and surface-active sites, thereby showcasing notable SERS enhancement, commendable reproducibility, and enduring stability over the long term. Furthermore, the Na2Ti3O7@RF@Ag heterostructures demonstrate remarkable follow-up as first-order chemical kinetic and recyclable photocatalysts for the photodecomposition of an RhB solution under UV light irradiation. This result can be attributed to the enhanced inhibition of electron–hole pair recombination and increased surface-active sites.

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