Identification of Low Phytic Acid in a Maize Inbred Line and Primary Screening of Its Molecular Markers

Gao, Qing‐Hua; Meng, Yan; Zhang, Cui; Jia, Meng; Zhao, Lei; Hou, Mingming; Demin, Jin; Li, Xuejiao; Niu, Dongdong; Miao, Liu-Yang; Dou, Shi-Juan; Liu, Lijuan; Li, Liyun; Zhai, Wenxue; Liu, Guozhen

doi:10.3724/sp.j.1006.2013.00935

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“…We previously isolated a recessive maize mutant, Qi319, that had a dramatic reduction in grain phytic acid (65% lower than Chang 7-2). The germination rate of Qi319 seeds was > 75%, lower than the rate for most control lines of inbred maize, however, no other negative effects were evident in plant development and seed maturation (Gao et al 2013).…”

Section: Characterizing the Genomic Sequence Of Qi319 Mips-1smentioning

confidence: 80%

“…Previously, we identified a recessive lpa mutation in a inbred line of maize named Qi319 which showed normal agronomic traits compared to other inbred lines, including Chang 7-2, Huang C and Zheng 58 (Gao et al 2013). To understand the function of the MIPS gene and the mechanisms of phytic acid accumulation in Qi319 seeds, the genomic DNA of the MIPS-1s gene and its promoter in Qi319 and control genotypes were cloned and sequenced to identify possible lesion(s) in CDS or regulatory region that might be associated with altered gene expression.…”

Section: Sequence Analysis Of Mips-1s Gene Isolated From the Low-phytmentioning

confidence: 99%

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Sequence analysis of MIPS-1s gene isolated from the low-phytic-acid inbred line of maize Qi319 and characterization of its expression patterns

Gao¹,

Zhang²,

Fan³

et al. 2015

Intern. Jour. Contemp. Microbiol.

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Myo-inositol 1-phosphate synthase (MIPS) is a pivotal enzyme that initiates the first step of phytic acid synthesis. In the present study the MIPS-1s gene isolated was from an inbred line of maize Qi319 containing low contents of phytic acid (lpa). Its sequence and expression patterns were also characterized. Results showed that the full-length genomic DNA sequence of the MIPS-1s gene is comprised of ten exons and nine introns, with a length of 3596 bp, and an open reading frame of 1533 bp that encodes a protein with 510 amino acids. A single amino acid substitution was found, compared to the sequence of the inbred maize line B73. In silico promoter analysis revealed various ciselements that might contribute to the differential regulation of MIPS-1s. The expression patterns of MIPS-1s proteins in leaves, roots, flowers and developing seeds of maize were also investigated using western blot. It showed similar patterns compared to the control line (Chang 7-2), suggesting that the MIPS-1s protein plays similar roles in the process of maize development and growth. Notably, it was found that the control line had a dramatically higher abundance of MIPS-1s protein than did Qi319, in the developing seeds and especially during the early period. This strongly indicates that higher levels of MIPS expression should precede and/or accompany synthesis and accumulation of phytate.

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Section: Characterizing the Genomic Sequence Of Qi319 Mips-1smentioning

confidence: 80%

Section: Sequence Analysis Of Mips-1s Gene Isolated From the Low-phytmentioning

confidence: 99%

Sequence analysis of MIPS-1s gene isolated from the low-phytic-acid inbred line of maize Qi319 and characterization of its expression patterns

Gao¹,

Zhang²,

Fan³

et al. 2015

Intern. Jour. Contemp. Microbiol.

Self Cite

View full text Add to dashboard Cite

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Identification of Low Phytic Acid in a Maize Inbred Line and Primary Screening of Its Molecular Markers

Cited by 1 publication

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Sequence analysis of MIPS-1s gene isolated from the low-phytic-acid inbred line of maize Qi319 and characterization of its expression patterns

Sequence analysis of MIPS-1s gene isolated from the low-phytic-acid inbred line of maize Qi319 and characterization of its expression patterns

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