Identification of lymphatic metastasis-associated genes in a metastatic ovarian cancer cell line

Abstract

Abstract. Ovarian cancer (OC) is the gynecological malignancy with the highest rate of mortality, and lymphatic metastasis is a critical factor in disease recurrence and prognosis. In the current study, the SKOV-3/LN403 cell line, which has high potential for lymph node metastasis was established as a result of four rounds of selection from retroperitoneal lymph nodes following intraperitoneal injections of SKOV-3 ovarian adenocarcinoma cells. In comparison to the parental SKOV-3 cell line, SKOV-3/LN403 has a higher rate of proliferation, is more invasive and exhibits greater resistance to paclitaxel. Subsequently, a novel animal model of OC lymphatic metastasis was developed with SKOV-3/LN403 cells and a high incidence of positive metastatic lymph nodes, peritoneal dissemination and bloody ascites were observed, which mimicked the clinical outcome of patients with OC. Analysis of the gene expression profiles of SKOV-3 and SKOV-3/LN403 cells identified several genes and pathways that may be involved in lymphatic metastasis of OC. The induction of focal adhesion kinase expression provides a potential therapeutic target for OC lymphatic metastasis.

Delineation of retroperitoneal metastatic lymph nodes in ovarian cancer with near-infrared fluorescence imaging

“…SKOV-3 cells, established from a human ovarian adenocarcinoma, were obtained from the Shanghai Institute of Cell Biology of the Chinese Academy of Sciences (Shanghai, China). The SKOV-3-LN subline, which has the potential to induce higher rates of lymphatic metastasis, peritoneal dissemination and bloody ascites, was established previously in our laboratory ( 17 ). Cells were grown in McCoy's 5A medium (Hyclone; GE Healthcare Life Sciences, Logan, UT, USA) and were supplemented with 10% (v/v) fetal bovine serum (Thermo Fisher Scientific, Inc., Waltham, MA, USA), 100 U/ml penicillin, and 100 µg/ml streptomycin.…”

Molecular Mechanisms Regulating Organ-Specific Metastases in Epithelial Ovarian Carcinoma

“…Another study was performed to characterize the patterns of expression of the ubiquitin-specific protease 7 (USP7); it was found that high expression of USP7 significantly correlated with lymph node metastases [ 116 ]. Upregulation of focal adhesion kinase (FAK) in EOC cells strongly correlated with incidence of lymph node metastases as well [ 117 ].…”