Identification of lymphocyte 5-HT3 receptor subtype and its implication in fish T-cell proliferation

Meyniel, Jean-Philippe; Khan, Naim-Akhtar; Ferrière, François; Deschaux, P

doi:10.1016/s0165-2478(97)02697-7

Cited by 27 publications

(12 citation statements)

References 26 publications

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“…Moreover, this stimulating effect could be blocked by administration of 20 µM metoclopramide, indicating that 5-HT may mediate an immunomodulatory effect in rainbow trout via activation of 5-HT 3 receptors. In a related study conducted by Meyniel and coworkers, 130 it was shown that increasing concentrations of 5-HT (0, 62.5, 125, 250 nM) inhibited the phytohemagglutinin-induced proliferation of T cells, presumably via 5-HT 3 receptors, as 2-methyl-5-HT had a similar effect in a concentration range of 0.5 to 100 µM. Analysis of cell cycle progression revealed that 5-HT and 2-methyl-5-HT prevented the transition of activated T cells from G 0 /G 1 -to S-phase during the cell cycle.…”

Section: -Ht and The Immune Systemmentioning

confidence: 91%

“…123 Using classical receptor binding studies with 2-methyl-5-HT as competing ligand, 5-HT 3 receptors were identified in lymphocytes of rainbow trout based on the displacement of [ 3 H]5-HT. 130 Further evidence for the presence of 5-HT 3 receptors in trout lymphocytes was provided by Ferrière and colleagues. 131 As increases in intracellular Ca 2+ levels have been associated with an induction of leukocyte proliferation in catfish, 132 the main objective of their experiments was to examine the effects of 5-HT on calcium signaling in peripheral blood lymphocytes of rainbow trout.…”

Section: -Ht and The Immune Systemmentioning

confidence: 95%

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Physiological Endpoints for Potential SSRI Interactions in Fish

Kreke

Dietrich

2008

Critical Reviews in Toxicology

115

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Selective serotonin reuptake inhibitors (SSRIs) are among the pharmaceutical compounds frequently detected in sewage treatment plant effluents and surface waters, albeit at very low concentrations, and have therefore become a focus of interest as environmental pollutants. These neuroactive drugs are primarily used in the treatment of depression but have also found broader use as medication for other neurological dysfunctions, consequently resulting in a steady increase of prescriptions worldwide. SSRIs, via inhibition of the serotonin (5-hydroxytryptamine, 5-HT) reuptake mechanism, induce an increase in extracellular 5-HT concentration within the central nervous system of mammals. The phylogenetically ancient and highly conserved neurotransmitter and neurohormone 5-HT has been found in invertebrates and vertebrates, although its specific physiological role and mode of action is unknown for many species. Consequently, it is difficult to assess the impact of chronic SSRI exposure in the environment, especially in the aquatic ecosystem. In view of this, the current knowledge of the functions of 5-HT in fish physiology is reviewed and, via comparison to the physiological role and function of 5-HT in mammals, a characterization of the potential impact of chronic SSRI exposure on fish is provided. Moreover, the insight on the physiological function of 5-HT strongly suggests that the experimental approaches currently used are inadequate if not entirely improper for routine environmental risk assessment of pharmaceuticals (e.g., SSRIs), as relevant endpoints are not assessed or impossible to determine.

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Section: -Ht and The Immune Systemmentioning

confidence: 91%

Section: -Ht and The Immune Systemmentioning

confidence: 95%

Physiological Endpoints for Potential SSRI Interactions in Fish

Kreke

Dietrich

2008

Critical Reviews in Toxicology

115

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“…cDNA microarray studies indicated the presence of both mRNA species in CD14ϩ monocytes (Table III) and activated CD4ϩ T cells (data not shown). Little is known about 5-HT 3 receptor function in blood monocytes, but activation of this receptor in lymphocytes may alter cellular proliferation (86). The identity of the cells expressing 5-HT 3 receptors in the intestine may include enterochromaffin cells that express 5-HT 3 receptors (9).…”

Section: -Ht 3b and 5-ht 3a Co-localize In Diverse Tissuesmentioning

confidence: 99%

The Pharmacological and Functional Characteristics of the Serotonin 5-HT3A Receptor Are Specifically Modified by a 5-HT3B Receptor Subunit

Dubin¹,

Huvar²,

D’Andrea³

et al. 1999

Journal of Biological Chemistry

264

232

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(9), and Ca 2ϩ influx into lymphocytes (10). 5-HT 3 receptor activation mediates emetic and inflammatory responses (11) and may contribute to pain reception, anxiety, cognition, cranial motor neuron activity, modulation of affect, and the behavioral consequences of drug abuse (Refs. 12 and 13; but see Ref. 14).The 5-HT 3A receptor subunit shares structural similarities with members of the superfamily of ligand-gated ion channels (15) and is thought to be a pentameric protein (16, 17) with multiple agonist and allosteric ligand binding sites (2, 11). Both native and recombinant 5-HT 3 receptors reveal rapid and cooperative activation by agonists and desensitization to prolonged application of 5-HT (reviewed in Refs. 1, 2, and 6). With a few exceptions, ligand-gated channels require the association of more than one kind of homologous subunit for function, and subunit composition determines the pharmacological (18) and kinetic (e.g. desensitization (19,20)) profile of heteromeric receptors. While the 5-HT 3A subunit expressed in heterologous systems functions efficiently as homomers, different voltagedependence, desensitization, and pharmacological properties between recombinant and native 5-HT 3 receptors suggest that native 5-HT 3 receptors may exist as heteromers (3,(21)(22)(23)(24)(25)(26)(27). Although the 5-HT 3A gene encodes splice variants in mouse (28, 29) and guinea pig (30), most of the characteristics of these variants are similar (29,31,32), and the subtle pharmacological differences (22, 31, 32) cannot completely account for the differences observed between recombinant homomers and native 5-HT 3 receptors. In fact, co-expression of both splice variants in oocytes could not reproduce the responses observed in the cell line from which the splice variants were cloned (22). Biochemical studies on porcine brain have revealed the existence of at least four proteins (52-71 kDa) closely associated with the 5-HT 3A subunit that may represent antigenically distinct channel subunits (33).The cloning of a 5-HT 3B subunit that modified the pharmacological and single channel characteristics of the 5-HT 3A subunit when co-expressed in heterologous expression systems was recently reported (34). We extend this report and provide evidence for the co-existence of both 5-HT 3B and 5-HT 3A receptor subunits in native cells, a requirement for heteromeric association. We show that 5-HT 3B has no effect on the function of ␣2␤2, ␣3␤4, ␣4␤2, and ␣7 nicotinic ACh receptors. Furthermore, the characterization of the pharmacology and function of heteromeric receptors in Xenopus oocyte and mammalian expression systems reported here differs from that described (34), and these differences have important consequences for the function of heteromers in vivo.* The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.The nucleotide sequence (s) 1 The abbreviations used...

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“…In the present study, while THC, DHC and PO activities were continuously depressed over time, the concentration of DA in the haemolymph increased to its highest level shortly after exposure to the lower salinity treatment, and recovered to the control level afterwards. It has been shown that various DA and 5-HT receptors exist on the lymphocyte membrane of human, mice and osteichthyes, and the expression of these BA receptors can modulate the immune reaction via changes of ion channel and intracellular ion concentration (Cao and Tan, 1998;Meyniel et al, 1997;Yang, 1997). However, such suppressive response by injection is in contrast to the classical immune response where the proPO system is triggered by proper invasive agents which lead to increased immunity (Bachere, 2000;Hernandez-Lopez, 1996;Perazzolo, 1997).…”

Section: Discussionmentioning

confidence: 99%

Responses of prophenoloxidase system and related defence parameters of Litopenaeus vannamei to low salinity

Pan

Xie

2010

J. Ocean Univ. China

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In this study, we investigated the effects of low salinity (26 and 21) on the prophenoloxidase (proPO) system and related defence parameters in the shrimp Litopenaeus vannamei. The results showed that low salinity induced a significant increase of dopamine (DA) concentration in haemolymph at 6 h of the experiment; on the other hand, total haemocyte count (THC), differential haemocyte count (DHC) and PO activity decreased over time to the lowest level at 24 h and remained low thereafter. Serine Protease (SP) and Proteinase Inhibitor (PI) activity in the two lower salinity treatments decreased to the lowest level at 12 and 24 h, respectively, and both recovered to the control level at 72 h. In contrast, α 2 -macroglobulin (α 2 M) activity in the two lower salinity treatments peaked at 24 h and then decreased to the control level at 72 h. Therefore, it may be concluded that stress-induced DA plays an important temporary role in neurotransmission and causes immune response in L. vannamei in adapting to salinity changes.

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Identification of lymphocyte 5-HT3 receptor subtype and its implication in fish T-cell proliferation

Cited by 27 publications

References 26 publications

Physiological Endpoints for Potential SSRI Interactions in Fish

Physiological Endpoints for Potential SSRI Interactions in Fish

The Pharmacological and Functional Characteristics of the Serotonin 5-HT3A Receptor Are Specifically Modified by a 5-HT3B Receptor Subunit

Responses of prophenoloxidase system and related defence parameters of Litopenaeus vannamei to low salinity

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