Identification of Main Paddy Rice Cultivars in Tochigi Prefecture by RAPD Analysis

The objective of this study was to classify bottle gourd (Lagenaria siceraria) varieties by random amplifi ed polymorphic DNA (RAPD) analysis and cluster analysis to select the varieties to be preserved. A total of 24 bottle gourd varieties including 5 varieties bred by Tochigi Prefecture were analyzed. Forty-four primers produced 31 scorable, variable RAPD markers within bottle gourd varieties. Only 4 polymorphic markers were produced in 10 varieties derived from Tochigi Prefecture. These results suggest that these 10 varieties are closely related with each other, but not with the introduced varieties. For preservation, the varieties derived from Tochigi Prefecture should be classifi ed into each phenotype and then representative varieties preserved. Introduced varieties should be preserved for those from wide areas.

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Diversity Analysis Using Agricultural Traits and SSR Markers of Wheat Germplasms

Shin

Kim

et al. 2022

Korean J. Breed. Sci.

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Breeders typically use core collections of a specific trait or core collections selected by a few genotypes to efficiently select breeding materials or to study functional genes. As a result, many accessions have been deemed redundant or duplicated and are no longer considered for use. This study aimed to investigate the agronomical characteristics and genetic diversity of 1,514 previously unused and unstudied wheat germplasms and to confirm their value as breeding materials using population structure analysis. The performance of these wheat germplasms was compared with that of 8,878 wheat breeding materials and 72 known Korean wheat cultivars. The results of agronomic trait diversity comparison showed that the germplasm populations used for breeding research did not completely encompass the unused germplasm populations. The agronomic traits of wheat germplasms varied greatly. ANOVA and PCA results revealed the greatest differences in growth habits (CV=0.339), panicle length (CV=0.330), and awn length (CV=0.296). To accurately assess the value of unused wheat germplasms as breeding materials, 106 SSR markers were extracted from the analysis of four representative Korean cultivars: Geuru, Geumgang, Uri, and Jokyoung. Among these, 24 SSR markers were chosen, and 129 wheat resources were subjected to population structure analysis, which revealed five subpopulations. Most of the 34 germplasms that originated in Korea were distributed in subpopulation 1 (18 accessions, 52.9%) and subpopulation 4 (12 accessions, 35.3%). Subgroups 2, 3, and 5 differed significantly in agronomic traits and genotypes, indicating their potential as breeding materials. The findings of this study could serve as a foundation for breeders and aid in the discovery and utilization of new wheat breeding materials.

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Identification of Main Paddy Rice Cultivars in Tochigi Prefecture by RAPD Analysis

Cited by 3 publications

References 9 publications

Ｒａｐｄ分析による栃木県を中心とした関東周辺地域のムギ類優良品種識別

Ｒａｐｄ分析による栃木県を中心とした関東周辺地域のムギ類優良品種識別

Classification of Bottle Gourd (<I>Lagenaria siceraria</I>) by RAPD Analysis

Diversity Analysis Using Agricultural Traits and SSR Markers of Wheat Germplasms

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