Identification of MAL2, a Novel Member of the MAL Proteolipid Family, Though Interactions with TPD52-like Proteins in the Yeast Two-Hybrid System

Wilson, Samuel H.; Bailey, Angela M.; Nourse, Craig; Matteï, Marie‐Geneviève; Byrne, Jennifer A.

doi:10.1006/geno.2001.6610

Cited by 83 publications

(85 citation statements)

References 44 publications

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“…Many of the other genes in this cluster have identified or suspected roles in the regulation of cell motility or tumor cell invasiveness (including DDR1, SPINT2, HRIHFB2122, TACSTD1, and WNT5A; Vogel et al 1997;Seipel et al 2001;Weeraratna et al 2002). Our findings provide further evidence of a role for these genes in regulation of cell motility, and may be particularly useful in elucidating the functions of less clearly described genes in this cluster, such as MAL2 (Wilson et al 2001). Another example is given in Figure 5B, a cluster of eight genes that includes seven genes known to play roles in sterol biosynthesis.…”

Section: Cluster Analysis Of the Confirmed Coexpression Networksupporting

confidence: 55%

Coexpression Analysis of Human Genes Across Many Microarray Data Sets

Lee¹,

Hsu²,

Sajdak³

et al. 2004

Genome Res.

705

625

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We present a large-scale analysis of mRNA coexpression based on 60 large human data sets containing a total of 3924 microarrays. We sought pairs of genes that were reliably coexpressed (based on the correlation of their expression profiles) in multiple data sets, establishing a high-confidence network of 8805 genes connected by 220,649 "coexpression links" that are observed in at least three data sets. Confirmed positive correlations between genes were much more common than confirmed negative correlations. We show that confirmation of coexpression in multiple data sets is correlated with functional relatedness, and show how cluster analysis of the network can reveal functionally coherent groups of genes. Our findings demonstrate how the large body of accumulated microarray data can be exploited to increase the reliability of inferences about gene function.[Supplemental material is available online at www.genome.org and http://microarray.cpmc.columbia.edu/tmm.]Gene expression microarray data is a form of high-throughput genomics data providing relative measurements of mRNA levels for thousands of genes in a biological sample. In the last few years, hundreds of laboratories have collected and analyzed microarray data, and the data are beginning to appear in public databases or on researchers' Web sites. These resources serve at least two purposes. One is as an archive of the data, which allows other researchers to confirm the results that have been published by the originator of the data. A second use is to permit novel analyses of the data, that go beyond what was envisioned or possible at the time of the original study. A novel analysis could involve just a single data set, or a meta-analysis of many data sets (where a "data set" is a group of microarrays that were collected together, and typically described as a group in a single publication). The combined analysis of multiple data sets forms the main topic of this paper.Most existing studies that have analyzed multiple independently collected microarray data sets have focused on differential expression, comparing two or more similar data sets to look for genes that distinguish different sets of samples (Breitling et al.

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Section: Cluster Analysis Of the Confirmed Coexpression Networksupporting

confidence: 55%

Coexpression Analysis of Human Genes Across Many Microarray Data Sets

Lee¹,

Hsu²,

Sajdak³

et al. 2004

Genome Res.

705

625

View full text Add to dashboard Cite

show abstract

“…MAL2 (also named T-cell differentiation protein 2) is a member of the MAL family of presumably raft-associated membrane proteins that was originally identified as binding partner of TPD52, a cancer-associated protein of unknown function (Wilson et al, 2001). MAL2 is predicted to contain four transmembrane domains and thus exhibits a topology reminiscent of synaptophysins, synaptogyrins, and SCAMPs (Sánchez-Pulido et al, 2002).…”

Section: Mal2 Is a Novel Integral Membrane Protein Preferentially Assmentioning

confidence: 99%

Quantitative Comparison of Glutamatergic and GABAergic Synaptic Vesicles Unveils Selectivity for Few Proteins Including MAL2, a Novel Synaptic Vesicle Protein

Grønborg¹,

Pavlos²,

Brunk³

et al. 2010

J. Neurosci.

171

168

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Synaptic vesicles (SVs) store neurotransmitters and release them by exocytosis. The vesicular neurotransmitter transporters discriminate which transmitter will be sequestered and stored by the vesicles. However, it is unclear whether the neurotransmitter phenotype of SVs is solely defined by the transporters or whether it is associated with additional proteins. Here we have compared the protein composition of SVs enriched in vesicular glutamate (VGLUT-1) and GABA transporters (VGAT), respectively, using quantitative proteomics. Of Ͼ450 quantified proteins, ϳ50 were differentially distributed between the populations, with only few of them being specific for SVs. Of these, the most striking differences were observed for the zinc transporter ZnT3 and the vesicle proteins SV2B and SV31 that are associated preferentially with VGLUT-1 vesicles, and for SV2C that is associated mainly with VGAT vesicles. Several additional proteins displayed a preference for VGLUT-1 vesicles including, surprisingly, synaptophysin, synaptotagmins, and syntaxin 1a. Moreover, MAL2, a membrane protein of unknown function distantly related to synaptophysins and SCAMPs, cofractionated with VGLUT-1 vesicles. Both subcellular fractionation and immunolocalization at the light and electron microscopic level revealed that MAL2 is a bona-fide membrane constituent of SVs that is preferentially associated with VGLUT-1-containing nerve terminals. We conclude that SVs specific for different neurotransmitters share the majority of their protein constituents, with only few vesicle proteins showing preferences that, however, are nonexclusive, thus confirming that the vesicular transporters are the only components essential for defining the neurotransmitter phenotype of a SV.

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“…Interestingly, liver does not express this MAL isoform, a finding consistent with the absence of direct apical delivery of single-TMD and GPI-anchored apical residents in hepatocytes. Recently, another MAL isoform was identified, MAL2, that is enriched in hepatic cells (Wilson et al, 2001;De Marco et al, 2002). In HepG2 cells treated with antisense oligonucleotides, transcytosis of pIgA was blocked from early endosomes to what appeared to be the SAC (De Marco et al, 2002).…”

Section: A General Component Of the Transcytotic Trafficking Machinermentioning

confidence: 99%

Transcytotic Efflux from Early Endosomes Is Dependent on Cholesterol and Glycosphingolipids in Polarized Hepatic Cells

Nyasae

Hubbard

Tuma

2003

MBoC

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We examined the role that lipid rafts play in regulating apical protein trafficking in polarized hepatic cells. Rafts are postulated to form in the trans-Golgi network where they recruit newly synthesized apical residents and mediate their direct transport to the apical plasma membrane. In hepatocytes, single transmembrane and glycolipid-anchored apical proteins take the "indirect" route. They are transported from the trans-Golgi to the basolateral plasma membrane where they are endocytosed and transcytosed to the apical surface. Do rafts sort hepatic apical proteins along this circuitous pathway? We took two approaches to answer this question. First, we determined the detergent solubility of selected apical proteins and where in the biosynthetic pathway insolubility was acquired. Second, we used pharmacological agents to deplete raft components and assessed their effects on basolateral-to-apical transcytosis. We found that cholesterol and glycosphingolipids are required for delivery from basolateral early endosomes to the subapical compartment. In contrast, fluid phase uptake and clathrin-mediated internalization of recycling receptors were only mildly impaired. Apical protein solubility did not correlate with raft depletion or impaired transcytosis, suggesting other factors contribute to apical protein insolubility. Examination of apical proteins in Fao cells also revealed that raft-dependent sorting does not require the polarized cell context.

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Identification of MAL2, a Novel Member of the MAL Proteolipid Family, Though Interactions with TPD52-like Proteins in the Yeast Two-Hybrid System

Cited by 83 publications

References 44 publications

Coexpression Analysis of Human Genes Across Many Microarray Data Sets

Coexpression Analysis of Human Genes Across Many Microarray Data Sets

Quantitative Comparison of Glutamatergic and GABAergic Synaptic Vesicles Unveils Selectivity for Few Proteins Including MAL2, a Novel Synaptic Vesicle Protein

Transcytotic Efflux from Early Endosomes Is Dependent on Cholesterol and Glycosphingolipids in Polarized Hepatic Cells

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