Identification of Marbling Gene Loci in Commercial Pigs in Canadian Herds

Meadus, William J.; Duff, Pascale; Juárez, M.; Roberts, Jordan C.; Zantinge, Jennifer L.

doi:10.3390/agriculture8080122

Cited by 7 publications

(4 citation statements)

References 31 publications

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“…At present, many studies have shown that IMF content of different breeds varies considerably (Garcia et al, 1986; Davoli et al, 2016). For instance, Chinese indigenous breeds are distinctively high in IMF content compared to main commercial breeds, among which the Duroc breed is the highest (Casellas et al, 2013; Meadus et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Single-Locus and Multi-Locus Genome-Wide Association Studies for Intramuscular Fat in Duroc Pigs

et al. 2019

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Intramuscular fat (IMF) is an important quantitative trait of meat, which affects the associated sensory properties and nutritional value of pork. To gain a better understanding of the genetic determinants of IMF, we used a composite strategy, including single-locus and multi-locus association analyses to perform genome-wide association studies (GWAS) for IMF in 1,490 Duroc boars. We estimated the genomic heritability of IMF to be 0.23 ± 0.04. A total of 30 single nucleotide polymorphisms (SNPs) were found to be significantly associated with IMF. The single-locus mixed linear model (MLM) and multiple-locus methods multi-locus random-SNP-effect mixed linear model (mrMLM), fast multi-locus random-SNP-effect efficient mixed model association (FASTmrEMMA), and integrative sure independence screening expectation maximization Bayesian least absolute shrinkage and selection operator model (ISIS EM-BLASSO) analyses identified 5, 9, 8, and 21 significant SNPs, respectively. Interestingly, a novel quantitative trait locus (QTL) on SSC 7 was found to affect IMF. In addition, 10 candidate genes ( BDKRB2 , GTF2IRD1 , UTRN , TMEM138 , DPYD , CASQ2 , ZNF518B , S1PR1 , GPC6 , and GLI1 ) were found to be associated with IMF based on their potential functional roles in IMF. GO analysis showed that most of the genes were involved in muscle and organ development. A significantly enriched KEGG pathway, the sphingolipid signaling pathway, was reported to be associated with fat deposition and obesity. Identification of novel variants and functional genes will advance our understanding of the genetic mechanisms of IMF and provide specific opportunities for marker-assisted or genomic selection in pigs. In general, such a composite single-locus and multi-locus strategy for GWAS may be useful for understanding the genetic architecture of economic traits in livestock.

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Section: Introductionmentioning

confidence: 99%

Single-Locus and Multi-Locus Genome-Wide Association Studies for Intramuscular Fat in Duroc Pigs

et al. 2019

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“…Several chromosomal regions (QTLs) and molecular markers (SNPs) are now providing insights into specific candidate gene(s) controlling growth, nutrient uptake, disease resistance, meat quality traits and fat metabolism [ 93 , 105 ]. However, it remains a major challenge to nutritionally sway existing differentially methylated sites where genes involved in lipid metabolism are found [ 118 ].…”

Section: Qtl Regions and Snps For Fat Metabolism And Imf Accretion In Pigsmentioning

confidence: 99%

Genes Related to Fat Metabolism in Pigs and Intramuscular Fat Content of Pork: A Focus on Nutrigenetics and Nutrigenomics

Malgwi

Halas

Grünvald

et al. 2022

Animals

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Fat metabolism and intramuscular fat (IMF) are qualitative traits in pigs whose development are influenced by several genes and metabolic pathways. Nutrigenetics and nutrigenomics offer prospects in estimating nutrients required by a pig. Application of these emerging fields in nutritional science provides an opportunity for matching nutrients based on the genetic make-up of the pig for trait improvements. Today, integration of high throughput “omics” technologies into nutritional genomic research has revealed many quantitative trait loci (QTLs) and single nucleotide polymorphisms (SNPs) for the mutation(s) of key genes directly or indirectly involved in fat metabolism and IMF deposition in pigs. Nutrient–gene interaction and the underlying molecular mechanisms involved in fatty acid synthesis and marbling in pigs is difficult to unravel. While existing knowledge on QTLs and SNPs of genes related to fat metabolism and IMF development is yet to be harmonized, the scientific explanations behind the nature of the existing correlation between the nutrients, the genes and the environment remain unclear, being inconclusive or lacking precision. This paper aimed to: (1) discuss nutrigenetics, nutrigenomics and epigenetic mechanisms controlling fat metabolism and IMF accretion in pigs; (2) highlight the potentials of these concepts in pig nutritional programming and research.

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“…Gene expression tools are often used to study metabolic pathways in farm animals, particularly of IMF. These works are highly valuable for clean meat research, as they are not aimed for regenerative medicine, but rather focus on meat traits, such as marbling (Lee et al, 2010;Sadkowski et al, 2014;Chen et al, 2015;Hudson et al, 2015;Meadus et al, 2018), myogenesis (Wang et al, 2005(Wang et al, , 2015Lee et al, 2014;Dai et al, 2016), and sensory qualities (Bernard et al, 2007;Hocquette et al, 2007;Bongiorni et al, 2016;Tizioto et al, 2016). Research on correlations between gene expression and meat quality has been recently summarized in several publications.…”

Section: Gene Expressionmentioning

confidence: 99%

Tissue Engineering for Clean Meat Production

Ben-Arye

Levenberg

2019

Front. Sustain. Food Syst.

201

134

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Increasing public awareness of foodborne illnesses, factory farming, and the ecological footprint of the meat industry, has generated the need for animal-free meat alternatives. In the last decade, scientists have begun to leverage the knowledge and tools accumulated in the fields of stem cells and tissue engineering toward the development of cell-based meat (i.e., clean meat). In tissue engineering, the physical and biochemical features of the native tissue can be mimicked; cells and biomaterials are integrated under suitable culture conditions to form mature tissues. More specifically, in skeletal muscle tissue engineering, a plurality of cell types can be co-cultured on a 3D scaffold to generate muscle fibers, blood vessels and a dense extracellular matrix (ECM). This review focuses on tissue engineering of skeletal muscle and the adjustments needed for clean meat development. We discuss the skeletal muscle structure and composition, and elaborate on cell types from farm animals that have the potential to recapitulate the muscle ECM, blood vessels, muscle fibers and fat deposits. We also review relevant biomaterials, primarily for fabricating scaffolds that can mimic the intramuscular connective tissues, as well as gene expression studies on the biological pathways that influence meat quality.

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Identification of Marbling Gene Loci in Commercial Pigs in Canadian Herds

Cited by 7 publications

References 31 publications

Single-Locus and Multi-Locus Genome-Wide Association Studies for Intramuscular Fat in Duroc Pigs

Single-Locus and Multi-Locus Genome-Wide Association Studies for Intramuscular Fat in Duroc Pigs

Genes Related to Fat Metabolism in Pigs and Intramuscular Fat Content of Pork: A Focus on Nutrigenetics and Nutrigenomics

Tissue Engineering for Clean Meat Production

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