Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals

Cadena, Elsa De La; Mahecha, Mateo; Velandia, Ana María; García-Betancur, Juan Carlos; Rojas, Laura; Porras, Jessica; Pallares, Christian; Villegas, María Virginia

doi:10.3390/antibiotics12030488

Cited by 3 publications

(1 citation statement)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other study showed that 83.3% of strains carrying mcr-1 gene showed fosfomycin susceptibility. 62 Beyond UTI as the main focus of fosfomycin prescription, fosfomycin also showed excellent diffusion to various tissues. Thus, it should be considered for managing various other types of infectious caused by MDR, XDR Enterobacterales.…”

Section: Discussionmentioning

confidence: 99%

Colistin Resistance among Enterobacterales Isolates: Underlying Mechanisms and Alternative Treatment Options

Makled,

Ali,

Mahmoud

et al. 2023

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Global dissemination of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Gram-negative bacteria (GNB) such as carbapenemase-producing Enterobacterales has resulted in reviving colistin as a final therapeutic alternative. Colistin resistance foretold a catastrophe. We aimed to detect the rates of carbapenems and colistin resistance among hospital-acquired Enterobacterales species, verify the underlying mechanisms and provide antibiogram for colistin-resistant isolates. The collected Enterobacterales isolates were tested for their antimicrobial susceptibility by the disk diffusion method and agar dilution was utilized for both imipenem and colistin. The production of ESβLs and carbapenemases was phenotypically assessed by the combined disk (CDT) and modified carbapenem inactivation (mCIM) tests, respectively. Possible attributes for colistin resistance were explored by detection of both plasmid- and efflux pump-mediated mechanisms. By multiplex PCR assay, carbapenem resistance (blaNDM-1 & blaOXA-48) and mobilized colistin-resistant-1 (mcr-1) genes were identified. A total of 160 Enterobacterales isolates were obtained of which 68.8% were MDR, 25% were XDR and 6.3% were pandrug-resistant (PDR) isolates with no statistically significant difference among Enterobacterales species (P> 0.05). Carbapenems resistance was detected in 41.3% (66/160) while colistin resistance was detected in 22% (36/160) of isolates. Proteus mirabilis expressed the highest rate of colistin resistance (100%; 16/16), followed by Enterobacter aerogenes (23.1%; 6/26), E. coli (13%; 6/46) and K.pneumoniae (11.1%; 8/72). One hundred percent (36/36) of colistin-resistant isolates proved efflux pump activity for colistin. However; only 2% (2/100) of tested Enterobacterales carried mcr-1 gene through molecular analysis. Colistin-resistant isolates exhibited variable susceptibility to the tested antimicrobial agents of which fosfomycin was the highest (94.1%). Efflux pump activity played a major role for colistin resistance among Enterobacterales species and fosfomycin could be a promising therapeutic option.

show abstract

Section: Discussionmentioning

confidence: 99%

Colistin Resistance among Enterobacterales Isolates: Underlying Mechanisms and Alternative Treatment Options

Makled,

Ali,

Mahmoud

et al. 2023

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

show abstract

Complete genome characterization of mcr-1-mediated colistin-resistant Escherichia coli from outpatients in Bulgaria

Sabtcheva,

Stoikov,

Donchev

et al. 2024

Enfermedades Infecciosas y Microbiología Clínica

View full text Add to dashboard Cite

Molecular Epidemiology of mcr-1-Positive Polymyxin B-Resistant Escherichia coli Producing Extended-Spectrum β-Lactamase (ESBL) in a Tertiary Hospital in Shandong, China

Liu,

Wang,

et al. 2024

Polish Journal of Microbiology

View full text Add to dashboard Cite

Escherichia coli, a rod-shaped Gram-negative bacterium, is a significant causative agent of severe clinical bacterial infections. This study aimed to analyze the epidemiology of extended-spectrum β-lactamase (ESBL)-producing mcr-1 -positive E. coli in Shandong, China. We collected 668 non-duplicate ESBL-producing E. coli strains from clinical samples at Shandong Provincial Hospital between January and December 2018, and estimated their minimum inhibitory concentrations (MICs) using a VITEK® 2 compact system and broth microdilution. Next-generation sequencing and bioinformatic analyses identified the mcr-1 gene and other resistance genes in the polymyxin B-resistant strains. The conjugation experiment assessed the horizontal transfer capacity of the mcr-1 gene. Of the strains collected, 24 polymyxin B-resistant strains were isolated with a positivity rate of 3.59% and among the 668 strains, 19 clinical strains carried the mobile colistin resistance gene mcr-1, with a positivity rate of approximately 2.8%. All 19 clinical strains were resistant to ampicillin, cefazolin, ceftriaxone, ciprofloxacin, levofloxacin, and polymyxin B. Seventeen strains successfully transferred the mcr-1 gene into E. coli J53. All transconjugants were resistant to polymyxin B, and carried the drug resistance gene mcr-1. The 19 clinical strains had 14 sequence types (STs), with ST155 (n = 4) being the most common. The whole-genome sequencing results of pECO-POL-29_mcr1 revealed that no ISApl1 insertion sequences were found on either side of the mcr-1 gene. Our study uncovered the molecular epidemiology of mcr-1-carrying ESBL-producing E. coli in the region and suggested horizontal transmission mediated by plasmids as the main mode of mcr-1 transmission.

show abstract

Identification of mcr-1 Genes and Characterization of Resistance Mechanisms to Colistin in Escherichia coli Isolates from Colombian Hospitals

Cited by 3 publications

References 50 publications

Colistin Resistance among Enterobacterales Isolates: Underlying Mechanisms and Alternative Treatment Options

Colistin Resistance among Enterobacterales Isolates: Underlying Mechanisms and Alternative Treatment Options

Complete genome characterization of mcr-1-mediated colistin-resistant Escherichia coli from outpatients in Bulgaria

Molecular Epidemiology of mcr-1-Positive Polymyxin B-Resistant Escherichia coli Producing Extended-Spectrum β-Lactamase (ESBL) in a Tertiary Hospital in Shandong, China

Contact Info

Product

Resources

About