Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes

Klijn, N.; Weerkamp, Ah; Vos, Willem M. de

doi:10.1128/aem.57.11.3390-3393.1991

Cited by 242 publications

(107 citation statements)

References 20 publications

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“…The objective of this study was to investigate the value of primer-specific P C R for identification of clinical isolates of S. epidermidis. Primer-specific PCR allows the detection of small amounts of D N A extracted directly from bacterial colonies or from a standardized bacterial suspension [25]. Primer-specific PCR is much faster and more reliable for the identification of S. epidermidis than the commercially available API ID32 STAPH system, allowing the assay to be performed in about 3 h. Ribotyping analysis has been widely used for comparing strains from a large variety of species because of its stability and reproducibility [20].…”

Section: Discussionmentioning

confidence: 99%

“…The primer-specific P C R was performed using the primers p2 (5 '-AAGGAGGTGATCCAGCCGCA-3') and pSe (5'-ACTCTATCTCTAGAGGGGTCAG-3') as described earlier [24]. PCR products were obtained by amplification of DNA directly from bacterial lysate [25]. For Southern hybridization studies, a 1549-bp fragment of S. epidermidis 16s rRNA generated by P C R was labeled with digoxigenin 11-dUTP (Dig-11-dUTP) by the random priming procedure (Boehringer Mannheim).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of coagulase-negative staphylococci by primer-specific polymerase chain reaction and ribotyping

Gaszewska-Mastalarz

Bartoszewicz

Przondo-Mordarska

et al. 1998

Clinical Microbiology and Infection

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OBJECTIVE: To analyze, by primer-specific polymerase chain reaction (PCR) and ribotyping, coagulase-negative staphylococci (CNS). METHODS: Forty-five clinical isolates of CNS were identified by the API ID32 STAPH system and ribotyping. Additionally, primer-specific PCR was evaluated for identification of clinical strains of Staphylococcus epidermidis. RESULTS: Forty-five isolates of CNS from neonates with nosocomial bacteremia were studied. The results of the S. epidermidis-specific PCR were compared with those obtained using ribotyping and the API ID32 STAPH system. Excellent congruence was found between primer-specific PCR and ribotyping. Primer-specific PCR proved to be a fast and reliable method for the identification of S. epidermidis strains. According to the primer-specific PCR and ribotyping analysis, a few CNS isolates were found to be incorrectly identified by the API ID32 STAPH system. CONCLUSIONS: Primer-specific PCR is a fast and reliable method for the identification of S. epidermidis. Primer-specific PCR in combination with ribotyping is a promising approach for studying the epidemiology of S. epidermidis and other CNS species in hospital.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Characterization of coagulase-negative staphylococci by primer-specific polymerase chain reaction and ribotyping

Gaszewska-Mastalarz

Bartoszewicz

Przondo-Mordarska

et al. 1998

Clinical Microbiology and Infection

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“…Strain identification was performed by comparing the partial 16S rDNA sequence with those present in the Sequence Match of the Ribosomal Database Project II (RDP II), as described by Cole et al (2003) and (GTG) 5 genomic fingerprint analysis (Rademaker et al 2004). The DNA sequence of 16S rRNA genes (V1 to V3 variable regions) of the strains was determined after amplification as described elsewhere (Klijn et al 1991;Hoppe-Seyler et al 2003).…”

Section: Identification Of Strainsmentioning

confidence: 99%

Growth stimulation of Brevibacterium sp. by siderophores

et al. 2006

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Aims: To assess which types of siderophores are typically produced by Brevibacterium and how siderophore production and utilization traits are distributed within this genus. Methods and Results: During co‐cultivation experiments it was found that growth of B. linens Br5 was stimulated by B. linens NIZO B1410 by two orders of magnitude. The stimulation was caused by the production of hydroxamate siderophores by B. linens NIZO B1410 that enabled the siderophore‐auxotrophic strain Br5 to grow faster under the applied iron‐limited growth conditions. Different patterns of siderophore production and utilization were observed within the genus Brevibacterium. These patterns did not reflect the phylogenetic relations within the group as determined by partial 16S rDNA sequencing. Most Brevibacterium strains were found to utilize hydroxamate siderophores. Conclusions: Brevibacteria can produce and utilize siderophores although certain strains within this genus are siderophore‐auxotrophic. Significance and Impact of the Study: It is reported for the first time that brevibacteria produce and utilize siderophores. This knowledge can be utilized to stimulate growth of auxotrophic strains under certain conditions. Enhancing the growth rate of Brevibacterium is of importance for the application of this species, for example, for cheese manufacturing or for industrial production of enzymes or metabolites.

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“…4). Originally, both strains were classified in the subspecies lactis on phenotypic criteria but analysis of DNA-DNA reassociation [5], and 16S RNA sequences [104,105], direct gene sequence comparisons and Southern hybridization analysis with cloned chromosomal genes as probes [106] indicated that MG1363 and the closely related strains NCDO712, NCDO763 (ML3), NCDO505 (C2), LM2301 belong genotypically to the subspecies cremoris. The high degree of restriction polymorphism between IL1403 and MG1363 is consistent with the divergence of genomic sequences between L. lactis subsp, lactis…”

Section: Comparison Of the Genomic Mapsmentioning

confidence: 99%

Chromosome mapping in lactic acid bacteria

Bourgeois

Lautier

Ritzenthaler

1993

FEMS Microbiology Reviews

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The chromosome structure of lactic acid bacteria has been investigated only recently. The development of pulsed-field gel electrophoresis (PFGE) combined with other DNA-based techniques enables whole-genome analysis of any bacterium, and has allowed rapid progress to be made in the knowledge of the lactic acid bacteria genome. Lactic acid bacteria possess one of the smallest eubacterial chromosomes. Depending on the species, the genome sizes range from 1.1 to 2.6 Mb. Combined physical and genetic maps of several species are already available or close to being achieved. Knowledge of the genomic structure of these organisms will serve as a basis for future genetic studies. Macrorestriction fingerprinting by PFGE is already one of the major tools for strain differentiation, identification of individual strains, and the detection of strain lineages. The genome data resulting from these studies will be of general application strain improvement.

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Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes

Cited by 242 publications

References 20 publications

Characterization of coagulase-negative staphylococci by primer-specific polymerase chain reaction and ribotyping

Characterization of coagulase-negative staphylococci by primer-specific polymerase chain reaction and ribotyping

Growth stimulation of Brevibacterium sp. by siderophores

Chromosome mapping in lactic acid bacteria

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