Identification of methionine enkephalin in the bovine cornea by fast atom bombardment-mass spectrometry

Tinsley, Peter W.; Dass, Chhabil; Trimble, Joann; Desiderio, Dominic M.

doi:10.1016/0014-4835(90)90051-u

Cited by 7 publications

(3 citation statements)

References 7 publications

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“…[MetS]enkephalin; receptors; cell proliferation; cell migration; growth regulation; opioid antagonist; eye; visual system; wound healing; naltrexone THE CORNEAL EPITHELIUM contributes to maintaining the optical clarity of the cornea and provides a major protective barrier for the underlying cornea (8). The complete reepithelialization of the cornea after trauma or surgery is essential for the restitution of normal visual function (11).…”

mentioning

confidence: 99%

Opioid growth factor modulates corneal epithelial outgrowth in tissue culture

Zagon

Sassani

McLaughlin

1995

American Journal of Physiology-Regulatory, Integrative and Comp

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In addition to neuromodulation, endogenous opioid peptides serve as growth factors. To determine involvement of opioids in the homeostatic renewal and repair of the corneal epithelium, epithelial outgrowths from 3-mm explants of rabbit cornea were investigated. Blockade of opioid-receptor interaction by the potent opioid antagonist naltrexone (NTX) for 7 days significantly increased the extent of outgrowths and the number and labeling index (DNA synthesis) of epithelial cells, relative to control levels. Outgrowths exposed to the opioid growth factor (OGF) [Met5]enkephalin for 7 days were subnormal in extent and labeling index and displayed alterations in architectural pattern. The effects of OGF on epithelial outgrowth were blocked by concomitant exposure to the opioid antagonist naloxone; naloxone alone had no effect on growth at the concentration utilized. NTX and OGF were active in both serum-containing and serum-free cultures. Immunocytochemical investigations showed that both OGF and its opioid receptor zeta (zeta) were present in epithelial cells growing in control media. The results indicate that an endogenous opioid peptide and its receptor are present in mammalian corneal epithelium and serve to modulate cell proliferation, migration, and organization.

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mentioning

confidence: 99%

Opioid growth factor modulates corneal epithelial outgrowth in tissue culture

Zagon

Sassani

McLaughlin

1995

American Journal of Physiology-Regulatory, Integrative and Comp

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“…[88][89][90]94,95 Interestingly, OGF has been detected in the bovine corneal epithelium. 96,97 OGF and the z-receptor are also present in the normal rabbit corneal epithelium. 98 In 1995, Zagon et al performed a study to show the physiological role of endogenous opioid systems (opioid peptides and receptors) in the mammalian corneal epithelium in vitro using rabbit corneal explants.…”

Section: Hepatocyte Growth Factor Egf Kgf and Tgf-bmentioning

confidence: 96%

“…These include developing neurons and glia of the mammalian nervous system (including the retina), glial cells in culture, myocardial and epicardial cells in neonatal heart, neuronal and non‐neuronal cancer cells and cellular renewal in the epithelium of the adult tongue 88–90,94,95 . Interestingly, OGF has been detected in the bovine corneal epithelium 96,97 . OGF and the ζ‐receptor are also present in the normal rabbit corneal epithelium 98 …”

Section: Treatment Of Neurotrophic Corneal Ulcersmentioning

confidence: 99%

New therapeutic approaches in the treatment of diabetic keratopathy: a review

Abdelkader

Patel

McGhee

et al. 2011

Clinical &Amp; Experimental Ophthalmology

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The cornea is densely innervated, and the integrity of these nerve fibres is critical in maintaining the refractive and protective functions of the cornea. Many ocular and systemic diseases can adversely affect corneal sensory nerves and consequently impair their function, with vision loss being the inevitable consequence of severe corneal neurotrophic ulceration. However, current standard treatments regimens are often ineffective. Over the past three decades, the role of growth factors in maintaining the normal structure and function of the cornea, and in corneal epithelial healing, has become increasingly evident. Many preclinical and clinical trials have shown that growth factors and cytokines can significantly enhance epithelialization (epithelial proliferation and migration) and consequently accelerate wound healing. More recently, local/topical administration of insulin, naltrexone (opioid antagonist) and nicergoline (ergoline derivatives) were found to improve, and significantly increase, the corneal wound healing rate. This report reviews the major attributes of these growth factors and therapeutic agents that may be used in ameliorating impaired corneal wound healing, and presents a perspective on the potential clinical use of these agents as a new generation of ophthalmic pharmaceuticals for the treatment of diabetic keratopathy.

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