Identification of microbial species present in a pesticide dissipation process in biobed systems using typical substrates from southeastern Mexico as a biomixture at a laboratory scale

Góngora-Echeverría, Virgilio R.; Quintal-Franco, Carlos; Arena-Ortiz, María Leticia; Giácoman‐Vallejos, Germán; Ponce‐Caballero, Carmen

doi:10.1016/j.scitotenv.2018.02.082

Cited by 31 publications

(19 citation statements)

References 35 publications

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“…The amplicon analysis indicated the coexistence of representative phyla of bacteria into the biomixture. Similar with our observations, the predominant bacteria in biomixtures exposed to pesticides (atrazine, carborufan, diazinon, glyphosate and iprodione) were Proteobacteria, Actinobacteria and Firmicutes [9,32]. All these phyla, together with Bacteroidete are reported as predominant bacteria in the soil [33,34], which influence the microbial composition in the biomixture [32].…”

Section: Discussionsupporting

confidence: 89%

Pesticide-tolerant bacteria isolated from a biopurification system to remove commonly used pesticides to protect water resources

et al. 2020

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In this study, we selected and characterized different pesticide-tolerant bacteria isolated from a biomixture of a biopurification system that had received continuous applications of a pesticides mixture. The amplicon analysis of biomixture reported that the phyla Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria were predominant. Six strains grew in the presence of chlorpyrifos and iprodione. Biochemical characterization showed that all isolates were positive for esterase, acid phosphatase, among others, and they were identified as Pseudomonas, Rhodococcus and Achromobacter based on molecular and proteomic analysis. Bacterial growth decreased as both pesticide concentrations increased from 10 to 100 mg L-1 in liquid culture. The Achromobacter sp. strain C1 showed the best chlorpyrifos removal rate of 0.072-0.147 d-1 a half-life of 4.7-9.7 d and a maximum metabolite concentration of 2.10 mg L-1 at 120 h. On the other hand, Pseudomonas sp. strain C9 showed the highest iprodione removal rate of 0.100-0.193 d-1 a half-life of 4-7 d and maximum metabolite concentration of 0.95 mg L-1 at 48 h. The Achromobacter and Pseudomonas strains showed a good potential as chlorpyrifos and iprodione-degrading bacteria.

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Section: Discussionsupporting

confidence: 89%

Pesticide-tolerant bacteria isolated from a biopurification system to remove commonly used pesticides to protect water resources

et al. 2020

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“…Both fungi strains were able to remove high concentrations of ATZ, IPR, and CHL, pesticides with different chemical structures and chemical characteristics (Table 1) that confer them several degrees of toxicity and persistence in the environment. The organic biomixture used in BPS is a good source of microorganisms adapted to pesticides, as reported in other studies, including our previous results [12,13,15]. Recently, we reported that bacteria like the Achromobacter sp.…”

Section: Discussionsupporting

confidence: 72%

“…This technology is very efficient in pesticide removal, achieving high levels of degradation for different pesticides that are commonly applied in farms, even after repeated applications [12]. In BPS, many diverse microorganisms, including bacteria and fungi, form part of the biomixture [12,13]. Recent studies report that microbial consortia and bacteria-single strains isolated from a BPS can efficiently remove pesticides such as CHL, iprodione (IPR), ATZ [12], 2,4-Dichlorophenoxyacetic acid, carbofuran, diazinon, and glyphosate [14], while the fungus Lecanicillium saksenae isolated from a contaminated biomixture was able to remove pendimethalin [15].…”

Section: Introductionmentioning

confidence: 99%

Treatment of Pesticide-Contaminated Water Using a Selected Fungal Consortium: Study in a Batch and Packed-Bed Bioreactor

et al. 2021

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This study provides the basis for implementing a continuous treatment system for wastewater containing a pesticide mixture formed by atrazine, iprodione, and chlorpyrifos. Two fungal strains (Verticilium sp. H5 and Metacordyceps sp. H12) isolated from a biomixture of a biopurification system were able to remove different pesticide concentrations (10 to 50 mg L−1) efficiently from the liquid medium; however, the half-life of the pesticides was reduced and characterized by a T1/2 of 5.4 to 9.2 d for atrazine, 3.7 to 5.8 d for iprodione, and 2.6 to 2.9 d for chlorpyrifos using the fungal consortium. The immobilization of the fungal consortium in alginate bead was effective, with the highest pesticide removal observed using an inoculum concentration of 30% wv−1. The packed-bed reactor with the immobilized fungal consortium, which was operated in the continuous mode at different flow rates (30, 60, and 90 mL h−1), required approximately 10 d to achieve removal efficiency (atrazine: 59%; iprodione: 96%; chlorpyrifos: ~85%). The bioreactor was sensitive to flow rate fluctuations but was able to recover performance quickly. The pesticide metabolites hydroxyatrazine, 3,5-dichloroaniline, and 3,5,6-trichloro-2-pyridinol were produced, and a slight accumulation of 3,5,6-trichloro-2-pyridinol was observed. Nevertheless, reactor removal efficiency was maintained until the study ended (60 d).

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“…To date, although EFSA recommended to implement microbial endpoints, such as arbuscular mycorrhiza fungi, in pesticide environmental risk assessment (EFSA, 2010), there are still a limited number of studies evaluating side-effect of synthetic pesticides on soil fungal community (Borowik et al, 2017; Morrison-Whittle et al, 2017; Rivera-Becerril et al, 2017; Góngora-Echeverría et al, 2018) and almost none concerning biopesticides (Gopal et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…As it was previously observed, application of pesticides, even at the recommended field dose, can induce a reduction in the overall fungal community, that could impacted the ecosystem services in which fungi are involved (Sebiomo et al, 2011; Schlatter et al, 2018). On the other hand, pesticides can be used as carbon source by some fungi known to possess biodegradation abilities (Ikehata et al, 2004; Tortella et al, 2005; Coppola et al, 2011; Panelli et al, 2017; Góngora-Echeverría et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Impact of Leptospermone, a Natural β-Triketone Herbicide, on the Fungal Composition and Diversity of Two Arable Soils

et al. 2019

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Impact of leptospermone, a β-triketone bioherbicide, was investigated on the fungal community which supports important soil ecological functions such as decomposition of organic matter and nutrients recycling. This study was done in a microcosm experiment using two French soils, Perpignan (P) and Saint-Jean-de-Fos (SJF), differing in their physicochemical properties and history treatment with synthetic β-triketones. Soil microcosms were treated with leptospermone at recommended dose and incubated under controlled conditions for 45 days. Untreated microcosms were used as control. Illumina MiSeq sequencing of the internal transcribed spacer region of the fungal rRNA revealed significant changes in fungal community structure and diversity in both soils. Xylariales, Hypocreales, Pleosporales and Capnodiales (Ascomycota phyla) fungi and those belonging to Sebacinales, Cantharellales, Agaricales, Polyporales, Filobasidiales and Tremellales orders (Basidiomycota phyla) were well represented in treated soil microcosms compared to control. Nevertheless, while for the treated SJF a complete recovery of the fungal community was observed at the end of the experiment, this was not the case for the P treated soil, although no more bioherbicide remained. Indeed, the relative abundance of most of the saprophytic fungi were lower in treated soil compared to control microcosms whereas fungi from parasitic fungi included in Spizellomycetales and Pezizales orders increased. To the best of our knowledge, this is the only study assessing the effect of the bioherbicide leptospermone on the composition and diversity of the fungal community in soil. This study showed that leptospermone has an impact on α- and β-diversity of the fungal community. It underlines the possible interest of microbial endpoints for environmental risk assessment of biopesticide.

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Identification of microbial species present in a pesticide dissipation process in biobed systems using typical substrates from southeastern Mexico as a biomixture at a laboratory scale

Cited by 31 publications

References 35 publications

Pesticide-tolerant bacteria isolated from a biopurification system to remove commonly used pesticides to protect water resources

Pesticide-tolerant bacteria isolated from a biopurification system to remove commonly used pesticides to protect water resources

Treatment of Pesticide-Contaminated Water Using a Selected Fungal Consortium: Study in a Batch and Packed-Bed Bioreactor

Impact of Leptospermone, a Natural β-Triketone Herbicide, on the Fungal Composition and Diversity of Two Arable Soils

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