Identification of Microbiota for Activated Sludge Acclimated By Paper Mill Effluent Kraft E1 Bioremediation

Abstract

Conventional systems treating paper effluents and cellulose are quite efficient in the reduction of Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD). In general, there is primary stage clarification followed by secondary treatment, usually with a biological system (activated sludge). However, for the efficient degradation of some organic compounds in this effluent, adapted microorganism accomplish the treatment which are necessary resulting in effluents with low concentrations of recalcitrant compounds. There are two methods for microorganism acclimation: genes cloning or natural.In this work, the original biomass collected from a domestic sewage treatment plant was adapted for application in the bioremediation of paper mill effluent, Kraft E 1 . So, the aim of this work was verifying the correlation of protozoa organisms and physical-chemical parameters during the acclimation period.The acclimation of the biomass was verified mainly through the reduction of COD. The monitoring of biomass growth was followed by measurement of suspended solids and volatile suspended solids. Parameters such as pH, Dissolved Oxygen (DO) and temperature were also monitored daily. The biological study was accomplished microscopically through the identification of protozoa that are considered bioindicators of the activated sludge system. The biomass acclimation period was 39 days. The stabilization of the system was observed through constant rates of total COD (56%). The suspended solids at the beginning of the acclimation phase were from 1580 mg L -¹ to 3580 mg L -¹, indicating biomass growth. A small acclimation period was necessary to observe the diversity of protozoa, mainly free ciliates, but nematodes were also present, indicating poor sludge sediment. At the end of the acclimation phase predominantly rotifers, but some free ciliates, ciliated communities and fixed ciliated were observed, indicating a good purification capacity of the system. Within the organisms amoeba was observed and this indicates good quality to the final effluent. Then, it is possible to correlate the biota presence with the high capacity of COD removal from the activated sludge system. Journal of Bioremediation & Biodegradation

Biodegradation capabilities of acclimated activated sludge towards glyphosate: Experimental study and kinetic modeling

“…Biomass acclimation process to hardly biodegradable organic compounds is a critical step to induce microbial selection and physiological transformations of the metabolic pathways during the biodegradation process [33]. Biomass concentration has an important effect on the biological treatment performance of activated sludge [34], described by MLSS in this study.…”

Influence of wastewater treatment plants' operational conditions on activated sludge microbiological and morphological characteristics

“…[5] Seventy percent of the protozoa species in sludge are ciliates. [6][7][8] A c c e p t e d M a n u s c r i p t because of their ability to feed on bacteria and suspended particles and to induce flocculation.…”

Wastewater Management Techniques: A Review of Advancement on the Appropriate Wastewater Treatment Principles for Sustainability

“…Conventional Activated Sludge Process (CASP) System is the most common and oldest bio-treatment process used to treat municipal and industrial wastewater (Madoni 1994;Cordi et al, 2012;Mang and Li, 2010;Arun, 2011;Cordi et al, 2012). The process may also employ trickling filters, which are basins or tower filled with support media such as stones, plastic shapes, or wooden slats where wastewater is applied intermittently, or sometimes continuously, over the media (Wilson et al, 2015).…”