Identification of microRNA‐96‐5p as a postoperative, prognostic microRNA predictor in nonviral hepatocellular carcinoma

Matsui, Takeshi; Hamada-Tsutsumi, Susumu; Naito, Yutaka; Nojima, Masanori; Iio, Etsuko; Tamori, Akihiro; Kubo, Shoji; Ide, Tatsuya; Kondo, Yasuteru; Eguchi, Yuichiro; Komori, Atsumasa; Morine, Yuji; Shimada, Mitsuo; Utsunomiya, Tohru; Shirabe, Ken; Kimura, Koichi; Hiasa, Yoichi; Chuaypen, Natthaya; Tangkijvanich, Pisit; Naiki‐Ito, Aya; Takahashi, Satoru; Ochiya, Takahiro; Tanaka, Yasuhito

doi:10.1111/hepr.13674

Cited by 14 publications

(13 citation statements)

References 41 publications

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“…5 It has been thought that the advent of the ability to control hepatitis B, and virtually eradicate hepatitis C would reduce hepatocellular carcinoma but in reality, the incidence of hepatocellular carcinoma has not decreased much in Japan, due to a rapid increase in non-viral hepatocellular carcinoma cases caused by lifestylerelated diseases. 2 In this study, Matsui et al 6 identified microRNAs that are upregulated in cancerous tissues compared to non-cancerous tissues in non-viral hepatocellular carcinoma (NBNC-HCC), and also clarified the clinical significance of microRNAs that are upregulated in cancerous tissues.…”

Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 90%

“…Judging from the number of F I G U R E 1 Total 57 002 articles were found using two key words, "cancer" and "microRNA" in PubMed from 2003 to November 4, 2001. A similar search using the terms "microRNA" and "hepatocellular carcinoma" found 5642 articles published from 2006 to November 4, 2021 [Colour figure can be viewed at wileyonlinelibrary.com] papers on cancer and microRNAs, including hepatocellular carcinoma, is a field in which many researchers are interested, and the study by Matasui et al 6 is timely in this respect.…”

Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 99%

“…Matsui et al 6 focused their study on microRNAs that were upregulated in cancerous tissue compared to noncancerous tissue. Of note, upregulated miR-96-5p in cancer tissues increased recurrencefree survival by suppressing recurrence.…”

Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 99%

“…Induction of apoptosis has been described as one mechanism by which miR-96-5p inhibits cancer cell proliferation. 6 The reader will want to know what are the direct target genes of miR-96-5p. It would be of great interest to identify target genes that prevent apoptosis.…”

Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 99%

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Can microRNA‐96‐5p serve as a therapeutic molecule in the near future?

Masaki

Tani

Morishita

2022

Hepatology Research

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Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 90%

Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 99%

Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 99%

Section: Can Microrna-96-5p Serve As a Therapeutic Molecule In The Near Future?mentioning

confidence: 99%

See 2 more Smart Citations

Can microRNA‐96‐5p serve as a therapeutic molecule in the near future?

Masaki

Tani

Morishita

2022

Hepatology Research

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“…MiR-182-5p has been validated to have an essential role in liver-related diseases. MiR-182-5p is a high-priority miRNA in HCC, and is closely associated with early recurrence and overall survival in patients ( 21 , 22 ). Numerous studies have shown that miR-182-5p is dramatically overexpressed in HCC and could enhance the ability of migration, invasion, adhesion and proliferation of HCC cells via repressing multiple targeting genes, such as FOXO3a ( 21 ), Hepatitis C virus p7 trans-regulated protein 3 (P7TP3) ( 23 ), and regulator of calcineurin 1 (RCAN1) ( 24 ).…”

Section: Discussionmentioning

confidence: 99%

Integrated Analyses Identify Key Molecules and Reveal the Potential Mechanism of miR-182-5p/FOXO1 Axis in Alcoholic Liver Disease

Zuo

Zeng

et al. 2021

Front. Med.

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Background: Alcoholic liver disease (ALD) is one of the most common chronic liver diseases worldwide. However, the potential molecular mechanism in ALD development remains unclear. The objective of this work was to identify key molecules and demonstrate the underlying regulatory mechanisms.Methods: RNA-seq datasets were obtained from Gene Expression Omnibus (GEO), and key molecules in ALD development were identified with bioinformatics analysis. Alcoholic liver disease mouse and cell models were constructed using Lieber-DeCarli diets and alcohol medium, respectively. Quantitative real-time PCR and Western blotting were conducted to confirm the differential expression level. Dual-luciferase reporter assays were performed to explore the targeting regulatory relationship. Overexpression and knockdown experiments were applied to reveal the potential molecular mechanism in ALD development.Results: Between ALD patients and healthy controls, a total of 416 genes and 21 microRNAs (miRNAs) with significantly differential expression were screened. A comprehensive miRNA-mRNA network was established; within this network, the miR-182-5p/FOXO1 axis was considered a significant pathway in ALD lipid metabolism. Mouse and cell experiments validated that miR-182-5p was substantially higher in ALD than in normal livers, whereas the expression of FOXO1 was dramatically decreased by alcohol consumption (P < 0.05). Next, dual-luciferase reporter assays demonstrated that miR-182-5p directly targets the binding site of the FOXO1 3′UTR and inhibits its mRNA and protein expression. In addition, miR-182-5p was found to promote hepatic lipid accumulation via targeting the FOXO1 signaling pathway, and inhibition of the miR-182-5p/FOXO1 axis improved hepatic triglyceride (TG) deposition in ALD by regulating downstream genes involved in lipid metabolism.Conclusion: In summary, key molecules were identified in ALD development and a comprehensive miRNA–mRNA network was established. Meanwhile, our results suggested that miR-182-5p significantly increases lipid accumulation in ALD by targeting FOXO1, thereby providing novel scientific insights and potential therapeutic targets for ALD.

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Influence of AFP on surgical outcomes in non-B non-C patients with curative resection for hepatocellular carcinoma

et al. 2022

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To study the clinical and prognostic features of non-B non-C alpha-fetoprotein (AFP)(-)-hepatocellular carcinoma (HCC) (NBNC-AFP(-)-HCC) and the relationship between the prognostic features of HCC and hepatitis B virus surface antigen (HBsAg) status and AFP. We enrolled 227 patients who underwent hepatic resection for HCC between January 1998 and December 2007 at Sun Yat-sen University Cancer Center, all of whom were diagnosed with HCC by pathology. All patients were stratified into one of four groups (B-AFP(+)-HCC, B-AFP(-)-HCC, NBNC-AFP(+)-HCC, and NBNC-AFP(-)-HCC) according to AFP levels and HBsAg status. The clinicopathologic and survival characteristics of NBNC-AFP(-)-HCC patients were compared with those of all other three groups. Out of the 105 NBNC-HCC patients, 43 patients (40.9%) had AFP-negative HCC. There were some differences in factors between the B-AFP(+) and NBNC-AFP(-) patients, such as age, body mass index (BMI), diabetes, and ALT (P < 0.05). On univariate analysis, tumour size, secondary tumour, and portal invasion were prognostic factors for overall survival (OS) and disease-free survival (DFS) (P < 0.05). Cox multivariate regression analysis suggested that tumour size and tumour number (P < 0.05) were independent predictors. In addition, compared with the B-AFP(+)-HCC, B-AFP(-)-HCC, and NBNC-AFP(+)-HCC groups, the NBNC-AFP(-)-HCC patients had the best DFS (P < 0.05). Compared with the B-AFP(+)-HCC and NBNC-AFP(+)-HCC groups, the NBNC-AFP(-)-HCC patients had better OS (P < 0.05), and survival rates were similar to those of B-AFP(-)-HCC patients. NBNC-AFP(-)-HCC patients had a relatively favourable prognosis. It can serve as a useful marker in predicting the risk of tumour recurrence in the early stages.

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Identification of microRNA‐96‐5p as a postoperative, prognostic microRNA predictor in nonviral hepatocellular carcinoma

Abstract: Aim:The microRNA (miR) clusters miR-183/96/182 and miR-217/216a/216b are significantly upregulated in nonviral hepatocellular carcinoma (NBNC-HCC).

Cited by 14 publications

References 41 publications

Can microRNA‐96‐5p serve as a therapeutic molecule in the near future?

Can microRNA‐96‐5p serve as a therapeutic molecule in the near future?

Integrated Analyses Identify Key Molecules and Reveal the Potential Mechanism of miR-182-5p/FOXO1 Axis in Alcoholic Liver Disease

Influence of AFP on surgical outcomes in non-B non-C patients with curative resection for hepatocellular carcinoma

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