Identification of microRNA editing sites in clear cell renal cell carcinoma

Abstract

Clear cell renal cell carcinoma (ccRCC) is a malignant tumor originating from the renal tubular epithelium. Although the microRNAs (miRNAs) transcriptome of ccRCC has been extensively studied, the role of miRNAs editing in ccRCC is largely unknown. By analyzing small RNA sequencing profiles of renal tissues of 154 ccRCC patients and 22 normal controls, we identified 1025 miRNA editing sites from 246 pre-miRNAs. There were 122 editing events with significantly different editing levels in ccRCC compared to normal samples, which include two A-to-I editing events in the seed regions of hsa-mir-376a-3p and hsa-mir-376c-3p, respectively, and one C-to-U editing event in the seed region of hsa-mir-29c-3p. After comparing the targets of the original and edited miRNAs, we found that hsa-mir-376a-1_49g, hsa-mir-376c_48g and hsa-mir-29c_59u had many new targets, respectively. Many of these new targets were deregulated in ccRCC, which might be related to the different editing levels of hsa-mir-376a-3p, hsa-mir-376c-3p, hsa-mir-29c-3p in ccRCC compared to normal controls. Our study sheds new light on miRNA editing events and their potential biological functions in ccRCC.

Identification of prognostic RNA editing profiles for clear cell renal carcinoma

“…Through in-depth analysis of small RNA sequencing data from 154 patients with ccRCC and 22 normal control kidney tissues, a total of 1025 miRNA editing sites were identified from 246 precursor miRNAs. Compared with normal kidney tissue samples, 122 editing events with significantly different editing levels were found in ccRCC, including two A-to-I editing events in the seed regions of has-mir-376a-3p and has-mir-376c-3p, and one C-to-U editing event detected in the seed region of has-mir-29c-3p, demonstrating the complexity and diversity of miRNA editing in ccRCC ( 23 ). The aforementioned related studies demonstrate the significant potential of RNA editing as a biomarker and therapeutic target in ccRCC.…”