Identification of microRNA-mRNA regulatory networks and pathways related to retinoblastoma across human and mouse

Tian, Rui; He, Zhaohui; Wang, Lu-Fei; Song, Meijiao; Liu, Lu; Zhang, Hui

doi:10.18240/ijo.2020.04.02

Cited by 3 publications

(1 citation statement)

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“…Although mutations in RB1 gene are responsible for most cases of RB, identification of miRNAs and lncRNAs that directly target RB1 in unilateral sporadic cases with RB1 + / + genotype tumors aid in uncovering novel non-genetic mechanisms of RB1 inactivation. Similar to our findings, previous studies reported that miRNAs, and lncRNAs negatively regulate translation of target cell cycle-related gene cyclins, E2F members, cell cycle inhibitors, and TGFB2 [ 86 , 87 , 88 ]. However, in vitro and in vivo functional studies must be done to validate/confirm the individual coding-noncoding interactions identified in RB sEVs.…”

Section: Discussionsupporting

confidence: 92%

Comprehensive Analysis of Serum Small Extracellular Vesicles-Derived Coding and Non-Coding RNAs from Retinoblastoma Patients for Identifying Regulatory Interactions

Manukonda

Yenuganti

Nagar

et al. 2022

Cancers

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The present study employed nanoparticle tracking analysis, transmission electron microscopy, immunoblotting, RNA sequencing, and quantitative real-time PCR validation to characterize serum-derived small extracellular vesicles (sEVs) from RB patients and age-matched controls. Bioinformatics methods were used to analyze functions, and regulatory interactions between coding and non-coding (nc) sEVs RNAs. The results revealed that the isolated sEVs are round-shaped with a size < 150 nm, 5.3 × 1011 ± 8.1 particles/mL, and zeta potential of 11.1 to −15.8 mV, and expressed exosome markers CD9, CD81, and TSG101. A total of 6514 differentially expressed (DE) mRNAs, 123 DE miRNAs, and 3634 DE lncRNAs were detected. Both miRNA-mRNA and lncRNA-miRNA-mRNA network analysis revealed that the cell cycle-specific genes including CDKNI1A, CCND1, c-MYC, and HIF1A are regulated by hub ncRNAs MALAT1, AFAP1-AS1, miR145, 101, and 16-5p. Protein-protein interaction network analysis showed that eye-related DE mRNAs are involved in rod cell differentiation, cone cell development, and retinol metabolism. In conclusion, our study provides a comprehensive overview of the RB sEV RNAs and regulatory interactions between them.

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Section: Discussionsupporting

confidence: 92%