Identification of microRNAs by small RNA deep sequencing for synthetic microRNA mimics to control Spodoptera exigua

Zhang, Yu Liang; Huang, Qi; Yin, Guohua; Lee, Samantha; Jia, Rui; Liu, Zhi Xin; Yu, Nana; Pennerman, Kayla K.; Chen, Xin; Guo, An

doi:10.1016/j.gene.2014.12.038

Cited by 27 publications

(22 citation statements)

References 34 publications

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“…Tens of miRNAs have been reported to be involved in metamorphosis development in insects, including Dme-let-7, Dme-miR-100, Dme-miR-125, Dme-miR-34, and Dme-miR-14 in D. melanogaster (Varghese and Cohen 2007;Caygill and Johnston 2008;Sokol et al 2008;Tennessen and Thummel 2008); Bmo-let-7 and Bmo-miR-281 in B. mori (Zhang et al 2009;Jiang et al 2013); the miR-2 family (Bge-miR-2, Bge-miR-13a, and Bge-miR-13b) in the cockroach B. germanica (Lozano et al 2015); NlumiR-8-5p and Nlu-miR-2a-3p in the rice planthopper N. lugens ); Sex-miR-4924 in the beet armyworm S. exigua (Zhang et al 2015); and Aael-miR-2942 in the mosquito A. albopictus (Puthiyakunnon et al 2013). Most targets of these miRNAs were hormone-inducible genes.…”

Section: Discussionmentioning

confidence: 99%

“…These two miRNAs target genes in the chitin biosynthesis pathway, the membranebound trehalase (Tre-2) and phosphoacetylglucosamine mutase (PAGM), inducing defective moulting and high mortality ). In the beet armyworm Spodoptera exigua, Sex-miR-4924 regulates the larval development and moulting by targeting chitinase 1 (Zhang et al 2015). Some lineage-specific miRNAs such as aael-miR-2942 in Aedes albopictus and miR-2768 in lepidopterans were also reported to have key roles in insect development (Puthiyakunnon et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Sun

Xiao

et al. 2017

RNA

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The accurate rise and fall of active hormones is important for insect development. The ecdysteroids must be cleared in a timely manner. However, the mechanism of suppressing the ecdysteroid biosynthesis at the right time remains unclear. Here, we sequenced a small RNA library of Chilo suppressalis and identified 300 miRNAs in this notorious rice insect pest. Microarray analysis yielded 54 differentially expressed miRNAs during metamorphosis development. Target prediction and in vitro dualluciferase assays confirmed that seven miRNAs (two conserved and five novel miRNAs) jointly targeted three Halloween genes in the ecdysteroid biosynthesis pathway. Overexpression of these seven miRNAs reduced the titer of 20-hydroxyecdysone (20E), induced mortality, and retarded development, which could be rescued by treatment with 20E. Comparative analysis indicated that the miRNA regulation of metamorphosis development is a conserved process but that the miRNAs involved are highly divergent. In all, we present evidence that both conserved and lineage-specific miRNAs have crucial roles in regulating development in insects by controlling ecdysteroid biosynthesis, which is important for ensuring developmental convergence and evolutionary diversity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Sun

Xiao

et al. 2017

RNA

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“…Drosophila Dnmt2 loss-of-fuction mutants showed decreased viability under stress conditions [43]. Dnmt2 is also essential for the efficient functioning of Dicer-2 (Dcr-2) in Drosophila [44]. Infection with Wolbachia can disrupt the genome-wide patterns of cytosine methylation in Aedes aegypti [45].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Jiang

Guo

et al. 2016

PLoS ONE

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Bombyx mori nucleopolyhedrosis virus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Since microRNAs (miRNAs) have been shown to play important roles in host-pathogen interactions, in this study we investigated the effects of BmNPV infection on silkworm microRNAs expression profile. To achieve this, we constructed and deep-sequenced two small RNA libraries generated from BmNPV infected and un-infected larvae. The results revealed that 38 silkworm miRNAs were differentially expressed after BmNPV infection. Based on the GO analysis, their predicted target genes were found to be involved in diverse functions such as binding, catalytic, virion and immune response to stimulus suggesting their potential roles in host-virus interactions. Using the dual-luciferase reporter assay, we confirmed that Bmo-miR-277-5p, up-regulated in BmNPV-infected larvae, targeted the B. mori DNA cytosine-5 methyltransferase (Dnmt2) gene which may play potential role in silkworm-BmNPV interaction. These results provide new insights into exploring the interaction mechanism between silkworm and BmNPV.

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“…Furthermore, nlu-miR-8-5p and nlu-miR-2a-3p levels were shown to be negatively regulated by 20E through the Broad Complex (BR-C). In Spodoptera exigua , commonly known as the beet army worm, sex-miR-4924 modulates larval development and molting, possibly through the regulation of the chitinase 1 [24]. Moreover, silencing of Dcr-1 in the migratory locust, Locusta migratoria , reduced miRNA content and disrupted two types of molt—nymph-nymph and nymph-adult [25].…”

Section: Growth and Developmentmentioning

confidence: 99%

Regulation of physiological processes by microRNAs in insects

Lucas

Zhao

Liu

et al. 2015

Current Opinion in Insect Science

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MicroRNAs (miRNAs) are small non-coding RNAs that function in gene regulatory processes in plants and animals by targeting sites within messenger RNA. In insects, miRNAs have been shown to regulate a variety of physiological processes throughout insect development, including molting, metamorphosis, oogenesis, embryogenesis, behavior and host-pathogen interactions. The roles of miRNAs in the model organism, Drosophila melanogaster, have been studied extensively due to the conserved nature of miRNA function among highly divergent species. However, seeking to understand miRNA function in non-drosophilid insect species has become a growing trend in insect science. Here, we highlight the recent discoveries regarding miRNA function in insect physiology and development.

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Identification of microRNAs by small RNA deep sequencing for synthetic microRNA mimics to control Spodoptera exigua

Cited by 27 publications

References 34 publications

Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Multiple miRNAs jointly regulate the biosynthesis of ecdysteroid in the holometabolous insects, Chilo suppressalis

Genome-Wide Analysis of Differentially Expressed microRNA in Bombyx mori Infected with Nucleopolyhedrosis Virus

Regulation of physiological processes by microRNAs in insects

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