Identification of miR-16-5p and miR-155-5p microRNAs differentially expressed in circulating leukocytes of pregnant women with polycystic ovary syndrome and gestational diabetes

Hocaoğlu, Meryem; Demirer, Selin; Karaalp, İlayda Loçlar; Kaynak, Esra; Attar, Erkut; Turgut, Abdülkadir; Karateke, Ateş; Kömürcü-Bayrak, Evrim

doi:10.1080/09513590.2020.1843620

Cited by 25 publications

(20 citation statements)

References 25 publications

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“…However, Cao et al did not investigate the utility of miR-16-5p for early identification of GDM in their study [ 57 ]. Two other studies investigated the expression of miR-16-5p in third-trimester samples of maternal leukocytes from Turkish pregnant women with pre-eclampsia and GDM [ 32 ] or from pregnant women with polycystic ovary syndrome and GDM [ 31 ]. They showed no differences in the expression of miR-16-5p between the different groups.…”

Section: Discussionmentioning

confidence: 99%

“…They showed no differences in the expression of miR-16-5p between the different groups. Of note, the GDM and control group in the two Turkish studies [ 31 , 32 ] were the same. Similarly, no differences in miR-16-5p levels were observed from second-third trimester serum samples from Mexican women with GDM [ 34 ] or serum sampled over a large part of gestation (13–31 weeks, median; 27 weeks) from South African women with GDM [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…The placenta expresses more than 500 miRNA species [ 22 , 23 ] attributable in part by two placenta expressed miRNA clusters; the chromosome 19 and 14 miRNA clusters; C19MC and C14MC, respectively. Dysregulated placenta-associated miRNAs [ 24 , 25 , 26 , 27 ] as well as altered circulating miRNA profiles [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 ] have been associated with GDM, mirroring physiological and pathological changes during pregnancy.…”

Section: Introductionmentioning

confidence: 99%

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The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Sørensen

Poppel

Desoyé

et al. 2021

Cells

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Early identification of gestational diabetes mellitus (GDM) aims to reduce the risk of adverse maternal and perinatal outcomes. Currently, no circulating biomarker has proven clinically useful for accurate prediction of GDM. In this study, we tested if a panel of small non-coding circulating RNAs could improve early prediction of GDM. We performed a nested case-control study of participants from the European multicenter ‘Vitamin D and lifestyle intervention for GDM prevention (DALI)’ trial using serum samples from obese pregnant women (BMI ≥ 29 kg/m2) entailing 82 GDM cases (early- and late- GDM), and 41 age- and BMI-matched women with normal glucose tolerance (NGT) throughout pregnancy (controls). Anthropometric, clinical and biochemical characteristics were obtained at baseline (<20 weeks of gestation) and throughout gestation. Baseline serum microRNAs (miRNAs) were measured using quantitative real time PCR (qPCR). Elevated miR-16-5p, -29a-3p, and -134-5p levels were observed in women, who were NGT at baseline and later developed GDM, compared with controls who remained NGT. A combination of the three miRNAs could distinguish later GDM from NGT cases (AUC 0.717, p = 0.001, compared with fasting plasma glucose (AUC 0.687, p = 0.004)) as evaluated by area under the curves (AUCs) using Receiver Operator Characteristics (ROC) analysis. Elevated levels of individual miRNAs or a combination hereof were associated with higher odds ratios of GDM. Conclusively, circulating miRNAs early in pregnancy could serve as valuable predictive biomarkers of GDM.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Sørensen

Poppel

Desoyé

et al. 2021

Cells

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“…Similarly, a decreased miR-155 expression was reported in platelets of diabetic patients [ 55 ]. Low plasma levels of miR-155 were also found in gestational DM [ 54 ]. Interestingly, patients with hepatitis C and insulin resistance also displayed lower serum levels of miR-155 compared to those with hepatitis C without insulin resistance, although hepatitis C infection was associated with manifold upregulation of this miRNA [ 41 ].…”

Section: Dysregulation Of Mir-155 In Dm: Findings From Clinical Studiesmentioning

confidence: 99%

Functional Role of miR-155 in the Pathogenesis of Diabetes Mellitus and Its Complications

Jankauskas

Gambardella

Sardu

et al. 2021

ncRNA

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Substantial evidence indicates that microRNA-155 (miR-155) plays a crucial role in the pathogenesis of diabetes mellitus (DM) and its complications. A number of clinical studies reported low serum levels of miR-155 in patients with type 2 diabetes (T2D). Preclinical studies revealed that miR-155 partakes in the phenotypic switch of cells within the islets of Langerhans under metabolic stress. Moreover, miR-155 was shown to regulate insulin sensitivity in liver, adipose tissue, and skeletal muscle. Dysregulation of miR-155 expression was also shown to predict the development of nephropathy, neuropathy, and retinopathy in DM. Here, we systematically describe the reports investigating the role of miR-155 in DM and its complications. We also discuss the recent results from in vivo and in vitro models of type 1 diabetes (T1D) and T2D, discussing the differences between clinical and preclinical studies and shedding light on the molecular pathways mediated by miR-155 in different tissues affected by DM.

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“…Additionally, the study showed that down-regulation of miR-143 mediates the metabolic switch from oxidative phosphorylation to aerobic glycolysis in the placenta of women ( 89 ), highlighting its critical role in GDM. Furthermore, several studies also reported miRNAs, such as miR-155-5p ( 90 ), miR-20a-5p ( 82 ), and miR-181a (in umbilical-cord blood cells) ( 91 ), were down-regulated and related to GDM. These studies suggested that down-regulated miRNAs also had a profound influence in the pathogenesis of GDM.…”

Section: Role Of Non-coding Rnas and Evs In Gdmmentioning

confidence: 99%

Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

et al. 2021

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Gestational diabetes mellitus (GDM) is defined as glucose intolerance with onset or first recognition in the second or third trimester of pregnancy. GDM has a considerable impact on health outcomes of the mother and offspring during pregnancy, delivery, and beyond. Although the exact mechanism regarding GDM remains unclear, numerous studies have suggested that non-coding RNAs, including long non-coding (lnc)RNAs, microRNAs, and circular RNAs, were involved in the pathogenesis of GDM in which they played vital regulatory roles. Additionally, several studies have revealed that extracellular vehicles also participated in the pathogenesis of GDM, highlighting their important role in this disease. Considering the lack of effective biomarkers for the early identification of and specific treatment for GDM, non-coding RNAs and extracellular vehicles may be promising biomarkers and even targets for GDM therapies. This review provides an update on our understanding of the role of non-coding RNAs and extracellular vehicles in GDM. As our understanding of the function of lncRNAs and extracellular vehicles improves, the future appears promising for their use as potential biomarkers and treatment targets for GDM in clinical practice.

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Identification of miR-16-5p and miR-155-5p microRNAs differentially expressed in circulating leukocytes of pregnant women with polycystic ovary syndrome and gestational diabetes

Cited by 25 publications

References 25 publications

The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

The Predictive Value of miR-16, -29a and -134 for Early Identification of Gestational Diabetes: A Nested Analysis of the DALI Cohort

Functional Role of miR-155 in the Pathogenesis of Diabetes Mellitus and Its Complications

Non-Coding RNAs and Extracellular Vehicles: Their Role in the Pathogenesis of Gestational Diabetes Mellitus

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