Identification of miRNAs associated with the mechanical response of hepatic stellate cells by miRNA microarray analysis

Yi, Suhong; Qin, Xia; Luo, Xu; Zhang, Yi; Liu, Zhijun; Zhu, Liang

doi:10.3892/etm.2018.6384

Cited by 4 publications

(9 citation statements)

References 35 publications

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“…The involvement of this c-miRNA in atherosclerosis, which is induced after postprandial lipemia as well as in muscle damage, is intriguing and deserves further investigation. In addition, miR-466b-5p is upregulated under liver disease conditions [42] and is also overexpressed in infection and lipopolysaccharide-induced inflammation [43]. For the other validated miRNAs, there is scarce information with relation to lipid metabolism.…”

Section: Resultsmentioning

confidence: 99%

Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge

et al. 2019

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Postprandial lipemia has many physiopathological effects, some of which increase the risk of cardiovascular disease. MicroRNAs (miRNAs) can be found in almost all biological fluids, but their postprandial kinetics are poorly described. We aimed to profile circulating miRNAs in response to a fat challenge. In total, 641 circulating miRNAs were assessed by real-time PCR in plasmas from mice two hours after lipid gavage. Mice with intestine-specific loss of Dicer were screened to identify potential miRNAs released by the intestine. A total of 68 miRNAs were selected for further validation. Ten circulating miRNAs were finally validated as responsive to postprandial lipemia, including miR-206-3p, miR-543-3p, miR-466c-5p, miR-27b-5p, miR-409-3p, miR-340-3p, miR-1941-3p, miR-10a-3p, miR-125a-3p, and miR-468-3p. Analysis of their possible tissues of origin/target showed an enrichment of selected miRNAs in liver, intestine, brain, or skeletal muscle. miR-206, miR-27b-5p, and miR-409-3p were validated in healthy humans. Analysis of their predicted target genes revealed their potential involvement in insulin/insulin like growth factor (insulin/IGF), angiogenesis, cholecystokinin B receptor signaling pathway (CCKR), inflammation or Wnt pathways for mice, and in platelet derived growth factor (PDGF) and CCKR signaling pathways for humans. Therefore, the current study shows that certain miRNAs are released in the circulation in response to fatty meals, proposing them as potential novel therapeutic targets of lipid metabolism.

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Section: Resultsmentioning

confidence: 99%

Postprandial Circulating miRNAs in Response to a Dietary Fat Challenge

et al. 2019

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“…HL-7702 cells were purchased from cell bank of typical culture preservation committee of chinese academy of Sciences. The cells were cultured at 37˚C in an atmosphere containing 5% CO 2 in Dulbecco's modified Eagle's medium (DMEM; Jiangsu Kaiji Biotechnology co., ltd.) containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific, Inc.) for different durations (2,6,12,24 and 48 h). The cell line was authenticated by short tandem repeat analysis.…”

Section: Methodsmentioning

confidence: 99%

“…cirrhosis of the liver can lead to sinusoidal portal hypertension, in which the pressure in the hepatic portal vein increases, which can cause these veins to become significantly dilated (5). Hepatocytes are epithelial cells that line the sinusoids, which are directly and indirectly affected by biomechanical factors, such as the pressure of the hepatic portal vein and the tension strain after the dilation of hepatic sinusoids (6). a number of studies have shown the important roles of biomechanical factors in the regulation of hepatocyte function.…”

Section: Introductionmentioning

confidence: 99%

Screening of differentially expressed miRNAs in tensile strain‑treated HepG2 cells by miRNA microarray analysis

Luo

Shen

et al. 2020

Mol Med Report

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cirrhosis and portal hypertension are associated with an increased risk of developing liver cancer. However, it is unknown how changes in the cellular mechanical microenvironment induced by portal hypertension affect the occurrence and development of liver cancer. The aim of this study was to determine the effect of tensile strain on the proliferation of a human liver cancer cell line (HepG2 cells) using methods such as flow cytometry, Cell Counting Kit-8 and 5-bromodeoxyuridine assays, and to examine the changes in microrna (mirna/mir) expression using microarray, reverse transcription-quantitative (rT-q)Pcr and bioinformatics analyses. it was demonstrated that cyclic tensile force promoted the proliferation of HepG2 cells. The most suitable research conditions were as follows: Tensile strain force loading amplitude 15%; frequency 1 Hz; and time 24 h. after loading the HepG2 cells under such conditions, the differentially expressed mirnas were screened out using an agilent Human mirna Microarray, identifying seven mirnas with significant differences (expression difference >2 times and P<0.05). a total of five were upregulated, including hsa-mir-296-5p, hsa-mir-6752-5p, hsa-mir-6794-5p, hsa-miR-6889-5p and hsa-miR-7845-5p; and two were downregulated, hsa-miR-4428 and hsa-miR-503-5p. The results of RT-qPCR also further confirmed the expression changes of these mirnas. Gene ontology and pathway analyses showed the involvement of these mirnas in numerous important physiological processes. These findings may provide novel mirna-based information, thus enhancing the understanding of the pathophysiological processes leading to liver cancer.

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“…7 Additionally, through an investigation on the mechanisms of miRNA profile in hematopoietic stem cells (HSCs), one team found that rno-miR-125b-2-3p was downregulated after tensile strain was applied to activated HSCs. 8 In light of a previous study, we developed and verified a novel classifier based on two miRNAs, miR-933 and miR-125b-2-3p. They are capable of predicting disease progression and the benefit of receiving standard first-line chemotherapy in advanced CRC.…”

Section: Introductionmentioning

confidence: 98%

“…In recent studies, hsa‐miR‐125b‐2‐3p was found to be linked with the pathological stage of CRC 7 . Additionally, through an investigation on the mechanisms of miRNA profile in hematopoietic stem cells (HSCs), one team found that rno‐miR‐125b‐2‐3p was downregulated after tensile strain was applied to activated HSCs 8 . In light of a previous study, we developed and verified a novel classifier based on two miRNAs, miR‐933 and miR‐125b‐2‐3p.…”

Section: Introductionmentioning

confidence: 99%