Identification of molecular markers for the flower type in the ornamental crop Calluna vulgaris

Borchert, Thomas; Hohe, Annette

doi:10.1007/s10681-009-9926-3

Cited by 9 publications

(4 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The bud-flowering phenotype in C . vulgaris has been identified as a mono-genic recessive trait; [5] however, until now its genetic basis is unclear.…”

Section: Introductionmentioning

confidence: 99%

AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)

et al. 2013

View full text Add to dashboard Cite

BackgroundCalluna vulgaris is one of the most important landscaping plants produced in Germany. Its enormous economic success is due to the prolonged flower attractiveness of mutants in flower morphology, the so-called bud-bloomers. In this study, we present the first genetic linkage map of C. vulgaris in which we mapped a locus of the economically highly desired trait “flower type”.ResultsThe map was constructed in JoinMap 4.1. using 535 AFLP markers from a single mapping population. A large fraction (40%) of markers showed distorted segregation. To test the effect of segregation distortion on linkage estimation, these markers were sorted regarding their segregation ratio and added in groups to the data set. The plausibility of group formation was evaluated by comparison of the “two-way pseudo-testcross” and the “integrated” mapping approach. Furthermore, regression mapping was compared to the multipoint-likelihood algorithm. The majority of maps constructed by different combinations of these methods consisted of eight linkage groups corresponding to the chromosome number of C. vulgaris.ConclusionsAll maps confirmed the independent inheritance of the most important horticultural traits “flower type”, “flower colour”, and “leaf colour”. An AFLP marker for the most important breeding target “flower type” was identified. The presented genetic map of C. vulgaris can now serve as a basis for further molecular marker selection and map-based cloning of the candidate gene encoding the unique flower architecture of C. vulgaris bud-bloomers.

show abstract

“…The bud-flowering phenotype in C . vulgaris has been identified as a mono-genic recessive trait; [5] however, until now its genetic basis is unclear.…”

Section: Introductionmentioning

confidence: 99%

AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)

et al. 2013

View full text Add to dashboard Cite

show abstract

“…SSCP is a very sensitive technique for the detection of single point mutations between different DNA fragments (Grieu et al [ 2004 ]; Muangprom et al [ 2005 ]). Recently, SSCP has been used in crop studies, such as marker assisted selection (Borchert and Hohe [ 2009 ]), comparative genomics (Castelblanco and Fregene [ 2006 ]), phylogenetics (Rousseau-Gueutin et al [ 2009 ]) and fitness effects of crop QTLs (Baack et al [ 2008 ]). Furthermore, the recent availability of rice genome sequences provides the opportunity to select genes/sequences distributed in the genome as SSCP markers.…”

Section: Introductionmentioning

confidence: 99%

Genetic structure of Thai rice and rice accessions obtained from the International Rice Research Institute

Chakhonkaen

Pitnjam

Saisuk

et al. 2012

Rice

View full text Add to dashboard Cite

BackgroundAlthough the genetic structure of rice germplasm has been characterized worldwide, few studies investigated germplasm from Thailand, the world’s largest exporter of rice. Thailand and the International Rice Research Institute (IRRI) have diverse collections of rice germplasm, which could be used to develop breeding lines with desirable traits. This study aimed to investigate the level of genetic diversity and structures of Thai and selected IRRI germplasm. Understanding the genetic structure and relationships among these germplasm will be useful for parent selection used in rice breeding programs.ResultsFrom the 98 InDel markers tested for single copy and polymorphism, 19 markers were used to evaluate 43 Thai and 57 IRRI germplasm, including improved cultivars, breeding lines, landraces, and 5 other Oryza species. The Thai accessions were selected from all rice ecologies such as irrigated, deep water, upland, and rainfed lowland ecosystems. The IRRI accessions were groups of germplasm having agronomic desirable traits, including temperature-sensitive genetic male sterility (TGMS), new plant type, early flowering, and biotic and abiotic stress resistances. Most of the InDel markers were genes with diverse functions. These markers produced the total of 127 alleles for all loci, with a mean of 6.68 alleles per locus, and a mean Polymorphic Information Content (PIC) of 0.440. Genetic diversity of Thai rice were 0.3665, 0.4479 and 0.3972 for improved cultivars, breeding lines, and landraces, respectively, while genetic diversity of IRRI improved and breeding lines were 0.3272 and 0.2970, respectively. Cluster, structure, and differentiation analyses showed six distinct groups: japonica, TGMS, deep-water, IRRI germplasm, Thai landraces and breeding lines, and other Oryza species.ConclusionsThai and IRRI germplasm were significantly different. Thus, they can be used to broaden the genetic base and trait improvements. Cluster, structure, and differentiation analyses showed concordant results having six distinct groups, in agreement with their development, and ecologies.Electronic supplementary materialThe online version of this article (doi:10.1186/1939-8433-5-19) contains supplementary material, which is available to authorized users.

show abstract

“…In contrast, other forms, such as the 'filled' or the 'multi-bracteate' types are less important. Previous investigations revealed the monogenic recessive inheritance of the 'bud-flowering' trait [ 3 ] that was described in literature for the first time (as far as known by the authors) in 1935 [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

'Who's who' in two different flower types of Calluna vulgaris (Ericaceae): morphological and molecular analyses of flower organ identity

et al. 2009

View full text Add to dashboard Cite

BackgroundThe ornamental crop Calluna vulgaris is of increasing importance to the horticultural industry in the northern hemisphere due to a flower organ mutation: the flowers of the 'bud-flowering' phenotype remain closed i.e. as buds throughout the total flowering period and thereby maintain more colorful flowers for a longer period of time than the wild-type. This feature is accompanied and presumably caused by the complete lack of stamens. Descriptions of this botanical particularity are inconsistent and partially conflicting. In order to clarify basic questions of flower organ identity in general and stamen loss in detail, a study of the wild-type and the 'bud-flowering' flower type of C. vulgaris was initiated.ResultsFlowers were examined by macro- and microscopic techniques. Organ development was investigated comparatively in both the wild-type and the 'bud-flowering' type by histological analyses. Analysis of epidermal cell surface structure of vegetative tissues and perianth organs using scanning electron microscopy revealed that in wild-type flowers the outer whorls of colored organs may be identified as sepals, while the inner ones may be identified as petals. In the 'bud-flowering' type, two whorls of sepals are directly followed by the gynoecium. Both, petals and stamens, are completely missing in this flower type. The uppermost whorl of green leaves represents bracts in both flower types.In addition, two MADS-box genes (homologs of AP3/DEF and SEP1/2) were identified in C. vulgaris using RACE-PCR. Expression analysis by qRT-PCR was conducted for both genes in leaves, bracts, sepals and petals. These experiments revealed an expression pattern supporting the organ classification based on morphological characteristics.ConclusionsOrgan identity in both wild-type and 'bud-flowering' C. vulgaris was clarified using a combination of microscopic and molecular methods. Our results for bract, sepal and petal organ identity are supported by the 'ABCDE model'. However, loss of stamens in the 'bud-flowering' phenotype is an exceptional flower organ modification that cannot be explained by modified spatial expression of known organ identity genes.

show abstract

Identification of molecular markers for the flower type in the ornamental crop Calluna vulgaris

Cited by 9 publications

References 32 publications

AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)

AFLP-based genetic mapping of the “bud-flowering” trait in heather (Calluna vulgaris)

Genetic structure of Thai rice and rice accessions obtained from the International Rice Research Institute

'Who's who' in two different flower types of Calluna vulgaris (Ericaceae): morphological and molecular analyses of flower organ identity

Contact Info

Product

Resources

About