Identification of monoclonal antibodies that cross-react with cytokines from different animal species

Pedersen, Lotte Gam; Castelruiz, Y.; Jacobsen, Stine; Aasted, Bent

doi:10.1016/s0165-2427(02)00139-3

Cited by 93 publications

(56 citation statements)

References 24 publications

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“…Two antibodies against bovine IFN-γ and IL-4 that have been shown previously to cross-react with mink (Pedersen et al, 2002 andJensen et al, 2003) also provided positive staining in our ICS of cells from ferret BAL and spleen ( Fig. 4A and C).…”

Section: Cross-reactive Anti-mouse Antibodies Recognize Intracellularsupporting

confidence: 70%

“…One reason for this is that ferret studies are hampered by a severe lack of immunological reagents. The ferret is a mustelid, and previous studies have identified cross-reactive monoclonal antibodies (mAbs) that bind mustelid proteins in mink (Aasted 1989, Danilenko et al, 1992, Jacobsen et al, 1993, Chen et al, 1997, Sager et al, 1997, Brodersen et al, 1998, Pedersen et al, 2002, Jensen et al, 2003, Saalmuller et al, 2005. These antibodies were originally generated against human, canine, ovine, and bovine cells.…”

Section: Introductionmentioning

confidence: 99%

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Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Rutigliano

Doherty

Franks

et al. 2008

Journal of Immunological Methods

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Influenza virus infections carry a high public health cost, and pandemics are potentially catastrophic. Though the ferret is generally regarded as the best model for human influenza, no reagents are available for the analysis of cellular immunity. We thus screened monoclonal antibodies (mAbs) made for identifying immune cells in other species to see if any were cross-reactive. Flow cytometric analysis of lymphocytes isolated from blood, spleen, and lung of normal and virus-infected ferrets indicated that several mouse mAbs bound to the corresponding antigens in ferrets. Typing bronchoalveolar lavage populations from pneumonic ferrets with mAb to human CD8 showed the massive CD8+ T cell enrichment characteristic of this infection in mice. The availability of this, and several other mAbs that showed cross-reactivity, should allow us to begin the dissection of cellmediated immunity in the ferret, which, at least from these early results, looks similar to the situation in mice.

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Section: Cross-reactive Anti-mouse Antibodies Recognize Intracellularsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Rutigliano

Doherty

Franks

et al. 2008

Journal of Immunological Methods

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“…The identification of mink cross-reactive mAbs against cytokines and staining methods for mink PBMCs have been described previously (Pedersen et al, 2002). Briefly, the cells were cultured for 4 h at 37 uC and in 5 % CO 2 , washed and treated with 4 % paraformaldehyde in PBS.…”

Section: Methodsmentioning

confidence: 99%

“…HM57; DakoCytomation), human CD8 (OKT8; ATCC) and human CD14 (code no. TÜ K4; DakoCytomation) (Pedersen et al, 2002)]. Rabbit anti-mouse IgG F(ab9) 2 conjugated to FITC (code no.…”

Section: Assessment Of Virus Infection In Pbmc and Organ Systemsmentioning

confidence: 99%

Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host

Nielsen

Søgaard

Jensen

et al. 2009

Journal of General Virology

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The mechanisms behind the in vivo virulence of immunosuppressive wild-type morbillivirus infections are still not fully understood. To investigate lymphotropism and host responses, we have selected the natural host model of canine distemper virus (CDV) infection in mink. This model displays multisystemic infection, similar to measles virus and rinderpest virus infections in their susceptible natural hosts. The wild-type CDVs investigated provoked marked virulence differences, inducing mild versus marked to severe acute disease. The mildly virulent wild-type virus induced transient lymphopenia, despite the development of massive infection of peripheral blood mononuclear cells (PBMCs) exceeding that determined for the highly virulent wild-type virus, indicating an inverse relationship between acute virulence and the extent of viraemia in the investigated wild-type viruses. Single-cell cytokine production in PBMCs was investigated throughout the acute infections. We observed Th1-and Th2-type cytokine responses beginning in the prodromal phase, and late inflammatory responses were shared between the wild-type infections. INTRODUCTIONMorbilliviruses are lymphotrophic and induce multisystemic infections in their highly susceptible natural hosts, causing disease with high morbidity and mortality worldwide. Members of the genus Morbillivirus within the family Paramyxoviridae include Measles virus (MV) of primates, Canine distemper virus (CDV) of carnivores and Rinderpest virus (RPV) of cloven-hoofed animals. Natural infections by all of these share common features, including lymphopenia and inhibition of lymphocyte proliferation in the acute phase (Griffin, 2007;Heaney et al., 2002;von Messling et al., 2003;Yanagi et al., 2006). Although live vaccines have effectively reduced the incidence of disease, the highly contagious morbilliviruses are still a major problem in human and veterinary medicine (Greene & Appel, 2006;Rima & Duprex, 2006). Like MV and RPV, CDV causes a typical acute infection characterized by virus replication in cells of the lymphoid system, with subsequent spread to tissues throughout the body. Acute clinical morbillivirus disease often takes a fatal course in highly susceptible natural hosts such as young dogs, mink and ferrets due to the highly multisystemic virulence, which includes central nervous system (CNS) involvement and profound immunosuppression, favouring opportunistic secondary pathogens (Appel, 1969;Blixenkrone-Møller, 1989;Kauffman et al., 1982;von Messling et al., 2006). Despite the clinically relevant immunosuppression, another hallmark of virulent morbillivirus infections is the induction of efficient and lifelong humoral and cellular immunity in hosts that eventually survive and appear to clear the virus (Appel et al., 1982;Griffin, 2007).The molecular events behind morbillivirus-induced perturbation of the immune system have been studied extensively and, based on in vitro studies, several mechanisms have been proposed to be involved (Heaney et al., 2002;Schneider-Schaulies & Dittme...

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“…For colour development, TMB peroxidase substrate (SureBlue; Kirkegaard & Perry Laboratories) was added; the reaction was stopped after 20-30 min by the addition of 0.1 M HCl and A 450 was read. The specificity of the interaction between GIF and IL-2 was verified by pre-incubating the IL-2-coated ELISA plate with an anti-ovine IL-2 mAb, 1E10 (a gift from Dr P. Wood, CSIRO Animal Health, Parkville, Australia; Pedersen et al, 2002), before incubating with GIF.…”

Section: Methodsmentioning

confidence: 99%

Conservation and variation of the parapoxvirus GM-CSF-inhibitory factor (GIF) proteins

Deane

Ueda

Wise

et al. 2009

Journal of General Virology

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The GIF protein of orf virus (ORFV) binds and inhibits the ovine cytokines granulocytemacrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). An equivalent protein has so far not been found in any of the other poxvirus genera and we therefore investigated whether it was conserved in the parapoxviruses. The corresponding genes from both the bovine-specific pseudocowpox virus (PCPV) and bovine papular stomatitis virus (BPSV) were cloned and sequenced. The predicted amino acid sequences of the PCPV and BPSV proteins shared 88 and 37 % identity, respectively, with the ORFV protein. Both retained the six cysteine residues and the WSXWS-like motif that are required for biological activity of the ORFV protein.However, an analysis of the biological activity of the two recombinant proteins revealed that, whilst the PCPV GIF protein bound to both ovine and bovine GM-CSF and IL-2 with very similar binding affinities to the ORFV GIF protein, no GM-CSF-or IL-2-binding activity was found for the BPSV protein.

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Identification of monoclonal antibodies that cross-react with cytokines from different animal species

Cited by 93 publications

References 24 publications

Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection

Lymphotropism and host responses during acute wild-type canine distemper virus infections in a highly susceptible natural host

Conservation and variation of the parapoxvirus GM-CSF-inhibitory factor (GIF) proteins

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