Identification of Morphological Character and Esterase Isozyme Pattern in Second-Generation Black Rice Plant Irradiated to Gamma Rays

Hartanti, R S; Putri, Tyas Aisyah; Zulfa, Fitriyah; Sutarno, .; Suranto, Suranto

doi:10.1088/1757-899x/193/1/012038

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…The highest panicle length was found in the Mustajab variety with a panicle length of 30.61 cm. Use of mutation technology by using Gamma radiation is able to show differences in rice plant morphology such as stem length, number of nodes, number of branches, leaf length and flowering period [11].…”

Section: Impact Of Strains On Growth and Yield Of Rice Plantsmentioning

confidence: 99%

Morpho-agronomic performance M5 mutant generation of aceh rice (Oryza sativa L.) of different organic fertilizer treatment

Safrizal,

Efendi,

Zakaria

2024

IOP Conf. Ser.: Earth Environ. Sci.

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The use of organic fertilizers is one of the efforts made to increase the levels of organic matter in the soil, especially paddy soil. This study aims to determine the effect of organic fertilizer of different treatments on the morpho-agronomic performance of the M5 mutant generation of Aceh rice (Oryza sativa L ). This research was conducted in ARRI, Aceh Rice Research Institute Banda Aceh, from April to July 2023. In this study, the experimental design used was a split-plot design with three replications where the subplots were nested in the main plot. The factors studied included the dose of organic fertilizer, which consisted of three levels: 0 ton ha-1, 5 ton ha-1, and 10 ton ha-1. The rice lines consisted of 5 groups: Mustajab Varieties, UM 14, UM 69, UM 8, and UM 89. The research results show that best of organic fertilizer was found with doses 10 tonnes ha-1 provides a significant effecton the number of tillers in harvest time 12,34 tillers. The best line/variety was found in the UM-89 treatment line with number of panicles 9,79 which was significantly different from the Mustajab variety which had a 7,88 panicles.

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Section: Impact Of Strains On Growth and Yield Of Rice Plantsmentioning

confidence: 99%

Morpho-agronomic performance M5 mutant generation of aceh rice (Oryza sativa L.) of different organic fertilizer treatment

Safrizal,

Efendi,

Zakaria

2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…Flowering is one of the characteristics that is affected by gamma-ray exposure. Gamma-ray exposure can cause changes in enzymes, proteins, and DNA related to flowering periods [10].…”

Section: Phenotype Of Black Ricementioning

confidence: 99%

Selection of M3 mutant strains from 200 gy gamma irradiation on Cempo Ireng

Nandariyah,

Sukaya,

Sutarno

et al. 2023

E3S Web Conf.

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Climate change is a serious issue that can disrupt crop production and pest resistance, especially in rice crops. To tackle climate change, plant breeding can be employed to develop new characteristics that are resistant, using physical mutagenesis like gamma irradiation. Cempo Ireng is one type of rice in Indonesia with plant properties such as tall plant, long harvesting periods, and susceptibility to brown planthopper attacks. The research aims to obtain information and select M3 mutant strains with properties of shorter plants, early harvesting, and brown planthopper resistance. The study was conducted from February-July 2022 in Klaten Regency, Central Java, Indonesia. The research material consisted of 3 mutant strains of black rice M3(GH8'), M3(GH51'), and M3(GH52'), with the control variety of Cempo Ireng. The experimental design used was a Randomized Complete Block Design without replication. Data were analyzed descriptively and used t-test, at a significance level of 5%. The results showed that M3 mutant black rice had better growth and yield components than the control plant. Brown planthopper attacks of M3 mutant strains decreased to 24% compared to the control.

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“…In recent years, the characterization of plants has been conducted not only based on morphological characters but also the isozyme banding pattern as reported by Premoli (2003) and Zolfghari et al (2010). The use of plant isozyme in the characterization of plants have been conducted widely (Rejon et al 2012;Houmani et al 2016;Hartanti et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

2018

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Hidayati NR, Suranto, Sajidan. 2018. Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes. Biodiversitas 19: 1683-1689. Aims of this research were to investigate the morphological character and isozyme banding patterns of Cucurbita moschata plants grown at three different altitudes. Samples in this study consisted of leaf, stem, and flowers. The morphological characters were conducted by direct observation in the field and analyzed descriptively as well as statically by one way ANOVA. The isozyme bands appearance of esterase and peroxidase of leaf samples were conducted using polyacrylamide gel electrophoresis (PAGE). Qualitative approach was used to analyze the presence and the absence of isozyme bands, while Retardation factor (Rf) was used to analyze quantitatively. The results showed that most plants grown at middle altitude (351-750 m asl.) were well-developed in terms of length of leaves, stems and flowers. Accordingly, the isozyme banding pattern of peroxidase was also found varied in plants grown at middle altitudes from which the presence of very unique bands was detected. Conversely, the band detected in plants grown at the lower and the highest altitudes was similar in term of band's number but it was different in the quality of the bands. Meanwhile, esterase isozyme banding pattern of plants grown at the lower and higher altitude had more bands than the middle altitude. Based on this result it is obvious that the isozyme data could be used to support in understanding the diversity morphological characters of plants grown in three different altitudes. This early result suggests that altitudes as a crucial factor in contributing the expression of isozyme appearance, which is useful for further pumpkin characterizations.

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Identification of Morphological Character and Esterase Isozyme Pattern in Second-Generation Black Rice Plant Irradiated to Gamma Rays

Cited by 4 publications

References 5 publications

Morpho-agronomic performance M5 mutant generation of aceh rice (Oryza sativa L.) of different organic fertilizer treatment

Morpho-agronomic performance M5 mutant generation of aceh rice (Oryza sativa L.) of different organic fertilizer treatment

Selection of M3 mutant strains from 200 gy gamma irradiation on Cempo Ireng

Morphological characteristics and isozyme banding patterns of Cucurbita moschata at different altitudes

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