Identification of Motile Sperm Domain–Containing Protein 2 as Regulator of Human Monocyte Migration

Mendel, Itzhak; Yacov, Niva; Salem, Yaniv; Propheta-Meiran, Oshrat; Ishai, Eti; Breitbart, Eyal

doi:10.4049/jimmunol.1601662

Cited by 12 publications

(14 citation statements)

References 19 publications

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“…S. agalactiae derived self-antigen, Lmb mimicked MOSPD2 was obtained after rigorous screening procedures explained in the previous study [1] . Recently, a group has shown the functional identification of MOSPD2 protein in the myeloid cells [89] . According to them, MOSPD2 is strictly localized on the plasma membrane of CD14+ human monocytes and neutrophils and hence, regulates monocyte migration.…”

Section: Discussionmentioning

confidence: 99%

Human MOSPD2: A bacterial Lmb mimicked auto-antigen is involved in immune infertility

Parida

2019

Journal of Translational Autoimmunity

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Autoantibody production is one of the leading factors of immune infertility, an autoimmune disease of the male reproductive system. The potential involvement of MHC-class II derived self-peptides against bacterial proteins in the antisperm antibody (ASA) production has been reported previously. Apparently, Streptococcus agalactiae has been considered as an important pathogen to impart infection-induced infertility in a bacteriospermia associated leukocytospermia (LCS/BS) state. Hence, the present study attempts to confirm S. agalactiae specific Laminin binding protein (Lmb) derived self-peptide (‘ KDSYTKKAKAFKKEA’) namely human Motile Sperm domain-containing protein 2 (MOSPD2) as an auto-antigen in LCS/BS condition. Semen samples were collected from infertile men with LCS/BS (n = 17) and their fertile counterparts (n = 10). Gram-positive bacteria were predominantly identified in the entire 17 LCS samples using culture method followed by 16S rDNA sequencing technique. TLRs 2 and 4 expression used as markers of immune response in spermatozoa and sperm dysfunction were elevated in the LCS/BS spermatozoa as compared to their fertile counterparts. A significant increase in oxidative stress indices i.e., protein carbonylation, lipid peroxidation and acridine orange test (AOT), was also observed in the LCS/BS spermatozoa. Spermatozoa lysate (both auto and heterologous), bacterial lysate (control) and synthesized MOSPD2 self-peptide were used to test their antigenicity against the autoantibodies by rocket immunoelectrophoresis (RIEP) assay. Seminal plasma from LCS/BS patients with S. agalactiae was used as the source of autoantibodies. Spermatozoa and bacteria lysate; and MOSPD2 self-peptide were able to bind autoantibodies in the seminal plasma. Besides, the self-peptide showed a dose dependent increase in the precipitation of antibody. T-cell epitope mapping of 48 Enterococcus faecalis and 91 Staphylococcus aureus surface proteins confirmed MOSPD2 as a global auto-antigen. Thus, augmentation of TLR expression in LCS/BS spermatozoa inferred MOSPD2 to be a putative immunogen. Altogether, these findings will delineate the significance of MOSPD2 auto-antigen in a bacteria derived immune infertility condition.

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Section: Discussionmentioning

confidence: 99%

Human MOSPD2: A bacterial Lmb mimicked auto-antigen is involved in immune infertility

Parida

2019

Journal of Translational Autoimmunity

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“…By tethering the ER and other organelles, MOSPD2 most likely functions in intracellular exchanges and communication. MOSPD2, which is expressed on the plasma membrane of human monocytes, was explored for its potential to regulate cancer cell migration and metastasis [ 55 , 56 ]. In our research, MOSPD2 had low expression in EBV-miR-BART-22 overexpressing cells.…”

Section: Discussionmentioning

confidence: 99%

Epstein–Barr virus-encoded microRNA BART22 serves as novel biomarkers and drives malignant transformation of nasopharyngeal carcinoma

et al. 2022

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Nasopharyngeal carcinoma (NPC) is an epithelial malignancy ubiquitously associated with Epstein–Barr virus (EBV). EBV generates various viral microRNAs (miRNAs) by processing the BHRF1 and BamHI A rightward (BART) transcripts. These BART miRNAs are abundantly expressed in NPC, but their functions and molecular mechanisms remain largely unknown. Our study found that the EBV-encoded microRNA BART-22 was significantly upregulated in NPC tissues and positively correlated with tumor progression. Furthermore, we found that EBV-miR-BART-22 was a significant predictor of poor prognosis in NPC. A reliable nomogram model to predict the preoperative overall survival (OS) of NPC patients was established. The area under the receiver operating characteristic (ROC) curve value for 5-year survival was 0.91. Elevated levels of EBV-miR-BART-22 significantly promoted the epithelial-mesenchymal transition (EMT) and metastasis of NPC cells in vivo and in vitro. We found that EBV-miR-BART-22 directly targets the 3′-UTR of MOSPD2 mRNA to promote the EMT and metastasis of NPC cells by activating the Wnt/β-catenin signaling pathway. Our findings provide a potential prognostic biomarker and new insight into the molecular mechanisms of NPC metastasis.

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“…MOSPD2 is also found in neutrophils, but not in lymphocytes. It is revealed that MOSPD2 is critical in regulating the inflammatory monocyte and neutrophil migration without activating ligands [ 103 ]. The monocyte from blood can rise macrophage in CNS, which is dominant in demyelination [ 104 ].…”

Section: Discussionmentioning

confidence: 99%

Bioinformatic analysis identifies potential key genes of epilepsy

et al. 2021

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Background Epilepsy is one of the most common brain disorders worldwide. It is usually hard to be identified properly, and a third of patients are drug-resistant. Genes related to the progression and prognosis of epilepsy are particularly needed to be identified. Methods In our study, we downloaded the Gene Expression Omnibus (GEO) microarray expression profiling dataset GSE143272. Differentially expressed genes (DEGs) with a fold change (FC) >1.2 and a P-value <0.05 were identified by GEO2R and grouped in male, female and overlapping DEGs. Functional enrichment analysis and Protein-Protein Interaction (PPI) network analysis were performed. Results In total, 183 DEGs overlapped (77 ups and 106 downs), 302 DEGs (185 ups and 117 downs) in the male dataset, and 750 DEGs (464 ups and 286 downs) in the female dataset were obtained from the GSE143272 dataset. These DEGs were markedly enriched under various Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms. 16 following hub genes were identified based on PPI network analysis: ADCY7, C3AR1, DEGS1, CXCL1 in male-specific DEGs, TOLLIP, ORM1, ELANE, QPCT in female-specific DEGs and FCAR, CD3G, CLEC12A, MOSPD2, CD3D, ALDH3B1, GPR97, PLAUR in overlapping DEGs. Conclusion This discovery-driven study may be useful to provide a novel insight into the diagnosis and treatment of epilepsy. However, more experiments are needed in the future to study the functional roles of these genes in epilepsy.

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Identification of Motile Sperm Domain–Containing Protein 2 as Regulator of Human Monocyte Migration

Cited by 12 publications

References 19 publications

Human MOSPD2: A bacterial Lmb mimicked auto-antigen is involved in immune infertility

Human MOSPD2: A bacterial Lmb mimicked auto-antigen is involved in immune infertility

Epstein–Barr virus-encoded microRNA BART22 serves as novel biomarkers and drives malignant transformation of nasopharyngeal carcinoma

Bioinformatic analysis identifies potential key genes of epilepsy

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