Identification of mRNA of the Inflammation-associated Proteins CXCL8, CXCR2, CXCL10, CXCR3, and β-Arrestin-2 in Equine Wounded Cutaneous Tissue: a Preliminary Study

Mund, Suzanne J K; Corbett, Charlotte A.; MacPhee, Daniel J.; Campbell, John J.; Honaramooz, Ali; Wobeser, Bruce; Barber, Spencer M.

doi:10.1016/j.jevs.2018.05.216

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…After binding, it can make the neutrophils chemotactic movements, and thus regulate inflammatory response (Mund et al. 2018 ). It is reported that MAPK14 plays a key role in activating the expression of pro-inflammatory genes in vascular smooth muscle cells, which is dependent on p65/NF-κB pathway (Wu et al.…”

Section: Discussionmentioning

confidence: 99%

Network pharmacology-based analysis of the mechanism of Saposhnikovia divaricata for the treatment of type I allergy

Wang

et al. 2022

Pharmaceutical Biology

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Context Saposhnikovia divaricata (Turcz.) Schischk (Apiaceae) (SD) has various pharmacological activities, but its effects on type I allergy (TIA) have not been comprehensively studied. Objective This study evaluates the treatment and molecular mechanisms of SD against TIA. Materials and methods The effective components and action targets of SD were screened using TCMSP database, and allergy-related targets of SD were predicted using GeneCards and OMIM database. The obtained target intersections were imported into David database for GO analysis, and used R software to perform KEGG analysis. The RBL-2H3 cells sensitised by DNP-IgE/DNP-BSA were treated with different concentrations of SD (root decoction, 0.5, 1, and 2 mg/mL), prim- O -glucosylcimifugin (POG, 10, 40, and 80 μg/mL) and the positive control drug–ketotifen fumarate (KF, 30 μM) for 12 h, then subjected to cell degranulation and qPCR analysis. Results Eighteen active compounds of SD and 38 intersection targets were obtained: TIA-related signal pathways mainly include calcium signal pathway, PI3K–Akt signal pathway and MAPK signal pathway. Taking the β-Hex release rate of the model group as the base, the release rate of SD and POG in high dose groups were 43.79% and 57.01%, respectively, which were significantly lower than model group ( p < 0.01), and significantly lower than KF group (63.83%, p < 0.01, p < 0.05). SD and POG could down-regulate the expression of related proteins in the Lyn/Syk, PI3K/AKT and MAPK signalling pathways. Discussion and conclusion Saposhnikovia divaricata could inhibit IgE-induced degranulation of mast cells, providing a scientific basis for further research and clinical applications of SD in TIA treatment.

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Section: Discussionmentioning

confidence: 99%

Network pharmacology-based analysis of the mechanism of Saposhnikovia divaricata for the treatment of type I allergy

Wang

et al. 2022

Pharmaceutical Biology

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“…The lower expression of CXCL8 seven days post injury in limb wounds is indicative of impaired neutrophil recruitment. However, the high expression of CXCL8 two weeks post injury in limb wounds may also explain the excessive angiogenesis typically seen in EGT (Mund et al, 2018).…”

Section: The Inflammatory Phasementioning

confidence: 99%