Identification of mRNAs with enhanced expression in ripening strawberry fruit using polymerase chain reaction differential display

Abstract

Fruit ripening is a complex developmental process that involves specific changes in gene expression and cellular metabolism. In climateric fruits these events are coordinated by the gaseous hormone ethylene, which is synthesized autocatalytically in the early stages of ripening. Nonclimacteric fruits do not synthesize or respond to ethylene in this manner, yet undergo many of the same physiological and biochemical changes associated with the production of a ripe fruit. To gain insight into the molecular determinants associated with nonclimacteric fruit ripening, we examined mRNA populations in ripening strawberry fruit using polymerase chain reaction (PCR) differential display. Five mRNAs with ripening-enhanced expression were identified using this approach. Three of the mRNAs appear to be fruit-specific, with little or no expression detected in vegetative tissues. Sequence analysis of cDNA clones revealed positive identities for three of the five mRNAs based on homology to known proteins. These results indicate that the differential display technique can be a useful tool to study fruit ripening and other developmental processes in plants at the RNA level.

cDNA Isolation and Gene Expression of the Maize Annexins p33 and p35

“…DNA (15 pg) was digested with either EcoRI, HindIII, or BamHI and the restriction fragments were sep- predicted from their root-tip cDNAs (ZMANNp33, ZMANNp35) and aligned against predicted annexin sequences for alfalfa (MSANN; Pirck et al, 1994), strawberry (FAANN; Wilkinson et al, 1995), and human annexin Xllla (HSANNXIII; Wice and Cordon, 1992). The numbers 1 to 4 above the top sequence refer to characteristic annexin repeat domains as applied to HSANNXlll (derived from Raynal and Pollard, 1994).…”

“…The longest cDNA clone was sequenced and its Comparison of annexin identities. The percentage identities were calculated pairwise for the amino acid sequences predicted from coding regions of full-length annexin cDNAs from maize (ZMANN33, ZMANN35), bell pepper (CAANN; Proust et al, 1996), and humans (HSANNXIII; Wice and Gordon, 1992), and partial cDNAs from alfalfa (MSANN; Pirck et al, 1994) and strawberry (FAANN; Wilkinson et al, 1995). The identities were calculated using the PALIGN program in PCGENE (Intelligenetics).…”

“…The open reading frame encodes a protein of 35.6 kD, which has a high degree of identity with partial annexin cDNAs from Medicago sativa (Pirck et al, 1994) and Fragaria ananassa (Wilkinson et al, 1995). The most similar nonplant annexin is the "intestine-specific" form of human annexin I, now classed as annexin XHIa (35% identity; see Figs.…”

Characterization and gene expression of an annexin during fruit development in Capsicum annuum

“…Up to now, this is the first full-length cDNA encoding a plant annexin. In the GenBank and EMBL nucleic acid database, incomplete cDNAs were described: a cDNA from strawberry [12] and the longest incomplete published cDNA from Medicago sativa [131.…”

Immunolocalization of a novel annexin‐like protein encoded by a stress and abscisic acid responsive gene in alfalfa

“…(Blackbourn et al, 1992;Boustead et al, 1989;Seals et al, 1994). Recently, a partial annexin cDNA sequence has been obtained from Medicago sativa (Pirck et al, 1994) and strawberry (Wilkinson et al, 1995), and full-length cDNA sequences from Capsicum annuum (Proust et al, 1996), Zea mays (Battey et al, 1996) and Arabidopsis thaliana (Gidrol et al, 1996). The predicted amino acid sequences of plant annexins show a high degree of similarity to each other, although they are distinct from the mammalian annexins.…”