Identification of MSEL and VLDV neurophysins in human pituitary gland

Identification of human neurophysins: complete amino acid sequences of MSEL- and VLDV-neurophysins.

“…Preliminary results on the NH2-terminal amino acid sequences of human neurophysin I or VLDV-neurophysin (5,6,10) and neurophysin II or MSEL-neurophysin (8,10) Extraction was usually carried out with fractions of 1 g of material. Each sample was extracted with 0.1 M HCl (50 ml/ g) for 4 hr at 40C.…”

“…Proteins are usually extracted by 0.1 M HCI at 4°C; after fractionation by molecular sieving, the crude neurophysins of Mr 10,000 are subjected to ion-exchange chromatography in order to isolate the different types of neurophysins (4,5). Purification also has been carried out by using continuous polyacrylamide gel electrophoresis (6)(7)(8). Neuro-physins are often termed neurophysin I, II, III, etc. according to their migrations to the anode, but this identification is confused by the presence of truncated forms.…”

“…2 Compared with bovine VLDV-neurophysin (Fig. 2) there are 11 substitutions-2 in the "inter-family variable" sequence [1][2][3][4][5][6][7][8][9]6 in the invariant sequence [namely, in positions 25 (Asn/Ser), 29 (Ala/Gly), 30 (Glu/Asp), 69 Although the COOH-terminal Gln-93 can be missing in some human samples, the five VLDV-neurophysins [namely, ox (28), pig (28), horse (29), rat (27), and man] characterized to date have 93 residues. In contrast to the MSEL-neurophysin family, no basic residue(s) is found near the COOH-terminal end, and, therefore, the putative processing enzyme that liberates VLDVneurophysin from a precursor may have a specificity different from that of the processing enzyme of MSEL-neurophysin precursors.…”

The neurophysin domain of human vasopressin precursor

“…In man, the two hormones have been isolated [5] using the neurophysin complex procedure [6] and the two neurophysins have been purified by polyacrylamide gel electrophoresis [7,8]. As a response to nicotine stimulation, arginine vasopressin and MSEL-neurophysin are selectively secreted in blood [7,8] and this co-secretion agrees with the presence of a common precursor similar to the one identified in ox. Furthermore a human neurohypophysial glycopeptide, homologous to the bovine glycopeptide [9], has recently been identified [IO].…”

“…In most placental mammals investigated, on the one hand two neurohypophysial hormones, arginine vasopressin and oxytocin have been identified [2,3], on the other hand two neurophysins, termed MSELand VLDV-neurophysins according to the nature of amino acids in positions 2,3,6 and 7, have been characterized ]2,4]. In man, the two hormones have been isolated [5] using the neurophysin complex procedure [6] and the two neurophysins have been purified by polyacrylamide gel electrophoresis [7,8]. As a response to nicotine stimulation, arginine vasopressin and MSEL-neurophysin are selectively secreted in blood [7,8] and this co-secretion agrees with the presence of a common precursor similar to the one identified in ox.…”