Identification of multiple nonphotochemical quenching processes in the extremophilic red alga Cyanidioschyzon merolae

Chiang, Yu-Hao; Huang, Yu-Jia; Fu, Han-Yi

doi:10.1007/s11120-022-00963-2

Cited by 1 publication

(1 citation statement)

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“…The F v /F m level was measured using a MultispeQ v2.0 spectrophotometer (PhotosynQ) with a method described by Chiang et al (2022) with minor modifications. In brief, cells were centrifuged at 1,000 × g for 6 min and resuspended in a fresh MA liquid medium with the Chl a concentration adjusted to 10 mg mL −1 .…”

Section: Determination Of the F V /F M Levelmentioning

confidence: 99%

Ascorbate peroxidase plays an important role in photoacclimation in the extremophilic red alga Cyanidiococcus yangmingshanensis

Wang

2023

Front. Plant Sci.

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IntroductionAcidothermophilic cyanidiophytes in natural habitats can survive under a wide variety of light regimes, and the exploration and elucidation of their long-term photoacclimation mechanisms promises great potential for further biotechnological applications. Ascorbic acid was previously identified as an important protectant against high light stress in Galdieria partita under mixotrophic conditions, yet whether ascorbic acid and its related enzymatic reactive oxygen species (ROS) scavenging system was crucial in photoacclimation for photoautotrophic cyanidiophytes was unclear.MethodsThe significance of ascorbic acid and related ROS scavenging and antioxidant regenerating enzymes in photoacclimation in the extremophilic red alga Cyanidiococcus yangmingshanensis was investigated by measuring the cellular content of ascorbic acid and the activities of ascorbate-related enzymes.Results and discussionAccumulation of ascorbic acid and activation of the ascorbate-related enzymatic ROS scavenging system characterized the photoacclimation response after cells were transferred from a low light condition at 20 μmol photons m–2 s–1 to various light conditions in the range from 0 to 1000 μmol photons m–2 s–1. The activity of ascorbate peroxidase (APX) was most remarkably enhanced with increasing light intensities and illumination periods among the enzymatic activities being measured. Light-dependent regulation of the APX activity was associated with transcriptional regulation of the chloroplast-targeted APX gene. The important role of the APX activity in photoacclimation was evidenced by the effect of the APX inhibitors on the photosystem II activity and the chlorophyll a content under the high light condition at 1000 μmol photons m–2 s–1. Our findings provide a mechanistic explanation for the acclimation of C. yangmingshanensis to a wide range of light regimes in natural habitats.

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Section: Determination Of the F V /F M Levelmentioning

confidence: 99%