Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53

Lederer, Franziska L.; Weinert, Ulrike; Günther, Tobias; Raff, Johannes; Weiß, Stephan; Pollmann, Katrin

doi:10.1099/mic.0.065763-0

Cited by 17 publications

(12 citation statements)

References 55 publications

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“…sphaericus JG-B53. Essential trace elements are able to cross the filter layer and reach the interior of this latter strain, which possesses outstanding recrystallization and metal-binding properties (Lederer et al 2013). The genome of Lys.…”

Section: Resistance To Other Adverse Environmental Conditionsmentioning

confidence: 98%

Role of S-layer proteins in bacteria

Gerbino

Carasi

Mobili

et al. 2015

World J Microbiol Biotechnol

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S-layers are paracrystalline bidimensional arrays of proteins or glycoproteins that overlay the cell surface of several genus and species of bacteria and archaea. As the outermost layer of several genus and species of microorganisms, S-layer proteins (SLP) are in direct contact with bacterial environment and thus may be involved in many of their surface properties, including adherence to various substrates, mucins and eukaryotic cells, aggregation and coaggregation with yeasts and other bacteria. In addition, SLP have been reported to be responsible for the bacterial protection against detrimental environmental conditions and to play an important role in surface recognition or as carriers of virulence factors. In this mini-review, we bring together the latest evidences about functional and mechanical properties of bacterial SLP from two different perspectives: (A) their role on bacterial adherence to different substrates and surfaces, and (B) their role as mechanical barriers in bacterial harmful environments.

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Section: Resistance To Other Adverse Environmental Conditionsmentioning

confidence: 98%

Role of S-layer proteins in bacteria

Gerbino

Carasi

Mobili

et al. 2015

World J Microbiol Biotechnol

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“…Pollmann et al [9] described a high similarity between the S-layer protein SlfB (L. sphaericus JG-A12) and SlfA (L. sphaericus NCTC 9602), with 98% sequence identity and a difference of only 3 amino acids / 13 nucleotides [46,47]. Similarly, Lederer et al [48] compared Slp1 (L. sphaericus JG-B53) to SlfB with a 67% sequence identity, as well as 65% for Slp1 to SlfA sequence identity.…”

Section: Sequence Comparison Of S-layer Proteins Slfb Slp1 Slfa Ssmentioning

confidence: 99%

“…These post-translational protein modifications might also influence binding properties. Recently, Lederer et al (2013) recently described various environmental factors that could impact the binding capacity of S-layer proteins [48]. In particular, the authors noted that cells can present different S-layer variants with specific abilities to cope with fast-changing environmental conditions.…”

Section: Sequence Comparison Of S-layer Proteins Slfb Slp1 Slfa Ssmentioning

confidence: 99%

S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water

Jung

Lakatos

Bengs

et al. 2019

Colloids and Surfaces B: Biointerfaces

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Bacterial surface layer proteins (S-layer) possess unique binding properties for metal ions. By combining the binding capability of S-layer proteins with the optical properties of gold nanoparticles (AuNP), namely plasmonic resonance, a colorimetric detection system for metal and metalloid ions in water was developed. Eight Slayer proteins from different bacteria species were used for the functionalization of AuNP. The thus developed biohybrid systems, AuNP functionalized with S-layer proteins, were tested with different metal salt solutions, e.g. Indium(III)-chloride, Yttrium(III)-chloride or Nickel(II)-chloride, to determine their selective and sensitive binding to ionic analytes. All tested S-layer proteins displayed unique binding affinities for the different metal ions. For each S-layer and metal ion combination markedly different reaction patterns and differences in concentration range and absorption spectra were detected by UV/vis spectroscopy. In this way, the selective detection of tested metal ions was achieved by differentiated analysis of a colorimetric screening assay of these biohybrid systems. A highly selective and sensitive detection of yttrium ions down to a concentration of 1.67 × 10 −5 mol/l was achieved with S-layer protein SslA functionalized AuNP. The presented biohybrid systems can thus be used as a sensitive and fast sensor system for metal and metalloid ions in aqueous systems.

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“…As shown in Figure 2, most investigated S-layers possess a higher binding capacity for toxic elements such as uranium or arsenic compared to intact cells. Especially the S-layer proteins and genes of the bacterial isolates Lysinibacillus sphaericus JG-A12 and L. sphaericus JG-B53, which were isolated from a uranium mining pile, have been extensively studied [56][57][58][59][60]. These proteins are able to bind high amounts of uranium (up to 20 mg U/g protein [61]) but also arsenic and precious metals such as Pd(II), Pt(II), and Au(III).…”

Section: Bacterial S-layersmentioning

confidence: 99%

Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources

et al. 2016

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Abstract:Microorganisms have developed various mechanisms to deal with metals, thus providing numerous tools that can be used in biohydrometallurgical processes. "Biomining" processes-including bioleaching and biooxidation processes-facilitate the degradation of minerals, accompanied by a release of metals. These processes are especially attractive for low-grade ores and are used on an industrial scale mainly for sulfidic ores. In biosorption processes, biomass or certain biomolecules are used to bind and concentrate selected ions or other molecules from aqueous solutions. Biosorptive materials can be an environmentally friendly and efficient alternative to conventional materials, such as ion exchange resins. Other interesting mechanisms are bioaccumulation, bioflotation, bioprecipitation, and biomineralisation. Although these processes are well-known and have been studied in detail during the last decades, the recent strong progress of biotechnologies (e.g., genetic engineering and molecule design), as well as their combination with novel developments in material sciences (e.g., nanotechnologies) facilitate new strategies for the application of biotechnologies in mineral processing. The article gives a summary of current activities in this field that are being performed in our group.

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Identification of multiple putative S-layer genes partly expressed by Lysinibacillus sphaericus JG-B53

Cited by 17 publications

References 55 publications

Role of S-layer proteins in bacteria

Role of S-layer proteins in bacteria

S-layer protein-AuNP systems for the colorimetric detection of metal and metalloid ions in water

Novel Biotechnological Approaches for the Recovery of Metals from Primary and Secondary Resources

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