Identification of N-glycan of alpha-fetoprotein by lectin affinity microarray

Chen, Pei; Liu, Yinkun; Kang, Xiaonan; Sun, Lu; Yang, Pengyuan; Tang, Zhao‐You

doi:10.1007/s00432-008-0357-7

Cited by 38 publications

(31 citation statements)

References 21 publications

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“…The gel-substrate lectin microarray was established in our laboratory, by which the altered glycosylation was capable of being analyzed in a rapid, sensitive and high-throughput manner. In a previous study, we screened the glycan profiles of a number of glycoprotein standards and compared the glycan profiles of AFP derived from various sources (9).…”

Section: Discussionmentioning

confidence: 99%

Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells

Kang

Wang

Chen

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. Human hepatocellular carcinoma (HCC) ranks second in cancer mortality in China; recurrence and metastasis have been the cause of the high mortality. Glycans on the cell surface play a pivotal role in tumor metastasis. The global alteration in the structure and composition of N-glycans during HCC metastasis remains unknown. To understand glycan alterations of glycoproteins by correlating the glycosyltransferase expression profile with glycan structure, we systematically used glycan profiling tools: glycogene microarray analyses of 115 genes, including glycotransferases, glycosidases and nuclear sugar transporters and lectin chips to investigate the glycan-related gene expression signatures in the high metastatic potential HCC cell line, HCCLM3, in comparison to the HCC cell line, Hep3B, with low metastatic potential. Of the 115 genes, 18 genes were up-regulated in high metastatic potential HCCLM3 cells in comparison to Hep3B cells, while 11 genes were down-regulated. The differentially expressed genes, such as ST3GalI, FUT8, β3GalT5, MGAT3 and MGAT5, were mainly involved in the synthesis of N-glycan and glycolipids, particularly the sialyl Lewis antigen. The results of the glycogene microarray analysis were further validated by quantitative real-time PCR analysis and lectin-based analysis. The differentially expressed glycogenes identified in this study may provide new insights and represent novel factors for investigating the functional role of cell surface carbohydrate-mediated HCC metastasis.

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Section: Discussionmentioning

confidence: 99%

Glycan-related gene expression signatures in human metastatic hepatocellular carcinoma cells

Kang

Wang

Chen

et al. 2011

Experimental and Therapeutic Medicine

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“…Recently, lectin microarray has been developed and employed as a multiplex glycan-lectin interaction analysis system for profiling either a pool of cell glycoproteins or a specific target glycoprotein [61][62][63][64][65]. Physical interactions (e.g., physical adsorption and supramolecular assembly) [66][67][68], covalent coupling [61,63,[69][70][71][72][73] and specific recognition reactions (e.g., biotin-avidin and DNA hybridization) [64,74] are three kinds of widely used methods to fabricate lectin microarrays. Hamachi and coworkers reported the construction of a novel fluorescent lectin array for saccharide detection using a supramolecular hydrogel matrix [67].…”

Section: Lectin Microarraymentioning

confidence: 99%

“…2) [55]. Lectin microarrays were used to recognize glycoconjugates [61,67,68,89]. Paulson and coworkers have demonstrated that a sialoside analogue microarray allows for the synthesis of sialoside analogues in a small quantity for subsequent screening for high affinity ligands of siglecs and other sialoside binding GBPs [90].…”

Section: Glycan-protein Interactions Analysismentioning

confidence: 99%

Microarray-Based Technology for Glycomics Analysis

Gao¹,

Ma²,

Liu³

2012

CCHTS

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In the post-genomic era, glycomics (the functional study of carbohydrates in living organisms) has come into the forefront of biological research because the interactions of glycoconjugates with proteins not only occur widely in biological processes of cells but also initiate infection of host cells by bacteria and viruses. Microarrays have been reportedly successful in carbohydrate-protein interaction as well as cellular surface glycan profiling. This review provides an overview of recent progress in the development of microarray-based techniques for glycomic studies. The fabrication, application and challenge/bottleneck of glycan/lectin microarrays have been summarized and discussed.

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“…Lectins are naturally occurring carbohydrate binding proteins which show some degree of specificity and have been shown to detect glycoproteins in less than 1 pg amounts [174]. Previous work has used lectins conjugated to chromatography resin (e.g [175]) or to magnetic beads for recovering glycan containing proteins from a complex mixture, for example serum [176].…”

Section: In-process Analysis Of Glycoproteinsmentioning

confidence: 99%