Identification of nanoparticles and their localization in algal biofilm by 3D-imaging secondary ion mass spectrometry

Benettoni, Pietro; Stryhanyuk, Hryhoriy; Wagner, Stephan; Kollmer, Felix; Osorio, Jairo Hernán Moreno; Schmidt, Matthias; Reemtsma, Thorsten; Richnow, Hans‐Hermann

doi:10.1039/c8ja00439k

Cited by 24 publications

(22 citation statements)

References 61 publications

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“…Delayed extraction is now widely used to image organic samples and lately was shown to achieve a 108 nm lateral resolution to visualize single particle in algal biofilms [43,44]. ToF-SIMS is a useful method for studying small molecules [45][46][47] and lipids in cells, especially in lipid-related diseases, such as cancer, Duchenne muscular dystrophy and atherosclerosis ( Figure 2) [48,49].…”

Section: Secondary Ion Mass Spectrometry (Sims)mentioning

confidence: 99%

Subcellular Chemical Imaging: New Avenues in Cell Biology

Decelle

Veronesi

Gallet

et al. 2020

Trends in Cell Biology

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To better understand the physiology and acclimation capability of a cell, one of the great challenges of the future is to access the interior of a cell and unveil its chemical landscape (composition and distribution of elements and molecules). Chemical imaging has greatly improved in sensitivity and spatial resolution to visualize and quantify nutrients, metabolites, toxic elements, and drugs in single cells at the subcellular level. This review aims at presenting the current potential of these emerging imaging technologies and guiding biologists towards a strategy for interrogating biological processes at the nanoscale. We also describe different solutions to combine multiple imaging techniques in a correlative way and provide perspectives and future directions for integrative subcellular imaging across different disciplines.

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Section: Secondary Ion Mass Spectrometry (Sims)mentioning

confidence: 99%

Subcellular Chemical Imaging: New Avenues in Cell Biology

Decelle

Veronesi

Gallet

et al. 2020

Trends in Cell Biology

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“…With a 100 µs period of primary pulse repetition, the sample surface was exposed to 0.05 pA of Bi 3 + probe and the secondary ion species were detected within 12-1200 Da range in Delayed-Extraction mode allowing for 120-250 nm lateral resolution and about 5000 MRP. 43 Analysis of 250×250 µm 2 size RoI’s were performed by rastering the Bi 3 + probe randomly in 2048×2048 px raster-pattern reaching 2 shots per pixel in 28 minutes of single analysis frame. After acquisition of each analysis frame, an area of 500 µm x500 µm or 800 µm x800 µm around the analysis crater was sputtered with either i) 75 nA of 1 keV Cs + or ii) 250 nA of 1 keV O 2 + beam in 200-400 frames for 3-7 minutes to analyze the complex heterogeneous sample in depth.…”

Section: Methodsmentioning

confidence: 99%

High-Resolution Chemical Mapping and Microbial Identification of Rhizosphere using Correlative Microscopy

Bandara

Schmidt

Davoudpour

et al. 2021

Preprint

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During the past decades, several stand-alone and combinatory methods have been developed to investigate the chemistry (i.e. mapping of elemental, isotopic and molecular composition) and the role of microbes in soil and rhizosphere. However, none of these approaches are currently capable of characterizing soil-root-microbe interactions simultaneously in their spatial arrangement. Here we present a novel approach that allows chemical and microbial identification of the rhizosphere at micro- to nano-meter spatial resolution. Our approach includes i) a resin embedding and sectioning method suitable for simultaneous correlative characterization of Zea mays rhizosphere, ii) an analytical work flow that allows up to six instruments/techniques to be used correlatively, and iii) data and image correlation. Hydrophilic, immunohistochemistry compatible, low viscosity LR white resin was used to embed the rhizosphere sample. We employed waterjet cutting and avoided polishing the surface to prevent smearing of the sample surface at nanoscale. Embedding quality was analyzed by Helium Ion Microscopy (HIM). Bacteria in the embedded soil was identified by Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization (CARD-FISH) to avoid interferences from high levels of auto fluorescence emitted by soil particles and organic matter. Chemical mapping of the rhizosphere was done by Scanning Electron Microscopy (SEM) with Energy-dispersive X-ray analysis (SEM-EDX), Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS), nanoSIMS and nano-focused Secondary Ion mass Spectrometry (nanoSIMS), and confocal Raman spectroscopy (μ-Raman). High-resolution correlative characterization by six different techniques followed by image registration shows that this method can meet the demanding requirements of multiple characterization techniques to chemically map the rhizosphere and identify spatial organization of bacteria. Finally, we presented individual and correlative workflows for imaging and image registration to analyze data. We hope this method will be a platform to combine various 2D analytics for an ample understanding of the rhizosphere processes and their ecological significance.

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“…LA-ICP-MS was used to determine the elemental distribution of Au NPs and salts residuals from the synthesis that are always present in the stock suspension of citrate coated Au NPs. 34 Fig. 6, the intensities in both the chemical images and transients (Fig.…”

Section: Analytes Deposited From Suspensionmentioning

confidence: 99%

“…As described previously, Mowital B 30 T can behave like a sponge and allow the transfer of water and solutes from the suspension drop into the polymer layer while keeping the analyte well attached. 34 However, Mowital B 30 T alone turned out not to be resistant to either acetone or ethanol (Table S-2). 48 Cross-linking, which was previously shown to enhance the adhesion and resistivity of the polymer layer, 35,36 yielded only partial improvement in this case (Table S-2).…”

Section: B Polymer Coatingmentioning

confidence: 99%

Surface cleaning and sample carrier for complementary high-resolution imaging techniques

Benettoni

Holbrook

et al. 2020

Biointerphases

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Nowadays, high-resolution imaging techniques are extensively applied in a complementary way to gain insights into complex phenomena. For a truly complementary analytical approach, a common sample carrier is required that is suitable for the different preparation methods necessary for each analytical technique. This sample carrier should be capable of accommodating diverse analytes and maintaining their pristine composition and arrangement during deposition and preparation. In this work, a new type of sample carrier consisting of a silicon wafer with a hydrophilic polymer coating was developed. The robustness of the polymer coating toward solvents was strengthened by cross-linking and stoving. Furthermore, a new method of UV-ozone cleaning was developed that enhances the adhesion of the polymer coating to the wafer and ensures reproducible surface-properties of the resulting sample carrier. The hydrophilicity of the sample carrier was recovered applying the new method of UV-ozone cleaning, while avoiding UV-induced damages to the polymer. Noncontact 3D optical profilometry and contact angle measurements were used to monitor the hydrophilicity of the coating. The hydrophilicity of the polymer coating ensures its spongelike behavior so that upon the deposition of an analyte suspension, the solvent and solutes are separated from the analyte by absorption into the polymer. This feature is essential to limit the coffee-ring effect and preserve the native identity of an analyte upon deposition. The suitability of the sample carrier for various sample types was tested using nanoparticles from suspension, bacterial cells, and tissue sections. To assess the homogeneity of the analyte distribution and preservation of sample integrity, optical and scanning electron microscopy, helium ion microscopy, laser ablation inductively coupled plasma mass spectrometry, and time-of-flight secondary ion mass spectrometry were used. This demonstrates the broad applicability of the newly developed sample carrier and its value for complementary imaging.

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Identification of nanoparticles and their localization in algal biofilm by 3D-imaging secondary ion mass spectrometry

Abstract: ToF-SIMS boundaries were pushed to enhance lateral resolution and mass resolving power for chemical imaging of nanoparticles in biological systems.

Cited by 24 publications

References 61 publications

Subcellular Chemical Imaging: New Avenues in Cell Biology

Subcellular Chemical Imaging: New Avenues in Cell Biology

High-Resolution Chemical Mapping and Microbial Identification of Rhizosphere using Correlative Microscopy

Surface cleaning and sample carrier for complementary high-resolution imaging techniques

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